7 results about "Phospholipid vesicles" patented technology

The application discloses an ultrasonic-temperature double-triggered double-liquid gas phase change contrast agent and a preparation method thereof. The preparation steps comprise the following steps: adding an isotonic buffer solution into a prepared composite phospholipid film, oscillating after ultrasonic dispersion, obtaining a targeted phospholipid vesicle dispersion; then high-pressure double-liquid co-loading is carried out, and the phase change temperature and the ultrasonic response are calibrated; filtering through a filter membrane, collecting the precipitate after centrifugation, adding a protective solution after washing with a PBS buffer solution, and sealing and storing to obtain the double-liquid gas phase change contrast agent. After the contrast agent enters the body, under the ultrasonic double triggering of the physiological temperature and an ultrasonic imaging instrument, the double-liquid gas synchronously phase changes into a gaseous state, drives the contrast agent to rapidly expand from a nano-sized particle into a micron-sized microbubble, realizes ultrasonic enhanced imaging, and has a significant clinical application value.

The invention provides a method for automatically preparing phospholipid vesicles, which comprises the following steps: spraying a first liquid by using a first automatic pipetting device with a liquid spraying function, the first liquid being a mixture of a first oil phase solution and a first water phase solution, the oil phase solution containing phospholipid, and the first water phase solution containing phospholipid; the first liquid is subjected to liquid spraying treatment to form a water-in-oil emulsion; the water-in-oil emulsion is dropwise added into second liquid through a second automatic pipetting device, the second liquid comprises an upper-layer oil phase and a lower-layer water phase, a phospholipid molecular layer is arranged between the upper-layer oil phase and the lower-layer water phase, water-in-oil emulsion liquid drops penetrate through the phospholipid molecular layer and enter the water phase to be dispersed, and a system containing phospholipid vesicles is formed; and separating to obtain the phospholipid vesicles. The preparation method disclosed by the invention is combined with an integrated automatic platform to complete the whole process of wrapping the biomolecules by the phospholipid vesicles, is simple in process flow and is suitable for wrapping the phospholipid vesicles containing various micromolecular dyes or biomacromolecules.

The invention belongs to the technical field of membrane modification, and particularly relates to a reverse osmosis membrane and a preparation method thereof. The preparation method comprises the following steps: firstly, carrying out hydration reaction on dispersion liquid containing carboxylated single-walled carbon nanotubes and a phospholipid layer to form phospholipid vesicle dispersion liquid in which the carbon nanotubes are vertically embedded; then taking an ultrafiltration membrane as a base membrane, breaking the phospholipid vesicles through suction filtration, forming a phospholipid bilayer structure on the surface of the base membrane, and vertically arranging the carbon nanotubes between the phospholipid bilayers to construct a phospholipid-carbon nanotube composite intermediate layer; and finally, forming a cross-linked polyamide layer on the surface of the composite intermediate layer through an interfacial polymerization reaction, and vertically fixing the carbon nanotubes in the membrane structure to complete the preparation of the reverse osmosis membrane. According to the method, the carbon nano tube is really used as a water channel, and the water molecule permeation rate of the reverse osmosis membrane is remarkably improved.

The invention provides a method for efficiently wrapping biomacromolecules with phospholipid vesicles, specifically, the method comprises the following steps: S1, adding an external phase aqueous solution into a first container, then adding an oil phase solution of phospholipid, and standing until a phospholipid monomolecular layer is formed; s2, adding the oil-phase solution of phospholipid and the internal-phase aqueous solution into a second container, and fully emulsifying the oil-phase solution of phospholipid and the internal-phase aqueous solution to form a water-in-oil emulsion; s3, adding the water-in-oil emulsion in the step S2 into the first container in which the phospholipid monomolecular layer is formed in the step S1, centrifuging, and discarding an upper-layer oil phase; wherein the osmotic pressure difference value of the external-phase aqueous solution and the internal-phase aqueous solution is within 100mOsmol / kg, and the internal-phase aqueous solution contains biomacromolecules.

The invention relates to the technical field of biological medicine, in particular to minoxidil hydrochloride entrapped mixed vesicle freeze-dried powder as well as a preparation method and application thereof.The minoxidil hydrochloride entrapped mixed vesicle freeze-dried powder comprises minoxidil hydrochloride entrapped mixed membrane vesicles, a freeze-drying protective agent, a vesicle stabilizer, a plasticizer, a pH buffer system and the balance of water, and the mixed membrane vesicles are composed of stem cell membrane vesicles and phospholipid vesicles, through an optimized preparation process including high-speed shearing, high-pressure homogenization and double-layer polycarbonate filter membrane circulating extrusion, uniform dispersion and stability of the vesicles are ensured. The freeze-drying protective agent prevents the vesicles from being broken, keeps the structural integrity, and is convenient for long-term storage and transportation. The pH buffer system adjusts the pH of the preparation to 5.5-6.5, irritation to skin is reduced, patient compliance is improved, the freeze-dried powder can act on a scalp alopecia area in a coating, non-woven fabric water infiltration and coverage, microneedle introduction or subcutaneous injection mode after being dissolved, and experimental results show that the preparation remarkably improves the hair papilla cell proliferation activity to 186%-425%.

The invention belongs to the technical field of nanofiltration membranes, and particularly relates to a high-performance phospholipid interlayer lithium-magnesium separation membrane as well as a preparation method and application thereof. The high-performance phospholipid interlayer lithium-magnesium separation membrane is prepared according to the following method: taking a polyethersulfone ultrafiltration membrane as a substrate, introducing a phospholipid interlayer, then performing interfacial polymerization through polyethyleneimine and trimesoyl chloride to form a polyamide separation layer, and finally performing heat treatment to obtain the high-performance phospholipid interlayer lithium-magnesium separation membrane. The phospholipid middle layer is a positively charged phospholipid double-layer membrane formed by spreading phospholipid vesicles on the surface of the polyethersulfone supporting layer. The phospholipid in the phospholipid vesicles is one of dioleoyl phosphatidylcholine, dioleoyl phosphatidyl ethanolamine and 1, 2-dioleoyl-3-trimethylamine propane hydrochloride. The transmittance of the lithium-magnesium separation membrane with the phospholipid interlayer is higher than 11L. M <-2 >. H <-1 >. Bar <-1 >, and the separation factor is greater than or equal to 29.5.

The application relates to a method for improving the encapsulation rate of a phospholipid vesicle-encapsulated polymer, and belongs to the fields of biotechnology and biochemistry. The application aims to solve the problem of low encapsulation rate of a polymer with relatively large molecular weight in the existing method for preparing a phospholipid vesicle. The method comprises the following steps: one, preparation of a PEG-Dextran two-water-phase solution; two, preparation of a phospholipid vesicle-encapsulated PEG-Dextran two-water-phase solution. The application is used for improving the encapsulation rate of a phospholipid vesicle-encapsulated polymer.