70 results about "Phospholipid vesicles" patented technology

A phospholipid vesicle such as a virus is to be rapidly separated (concentrated, roughly purified) and good detection (diagnosis) results can be obtained with suppressing inhibition of virus-denature, PCR inhibition, latex-aggregation inhibition, and the like. Moreover, the above operations are to be automated. A phospholipid vesicle is separated using a water-soluble cationic magnetic fine particle by a composite formation through a covalent bond or physical adsorption of a substance having a cationic functional group, a substance having a hydroxyl group, and a substance having magnetism.

The invention relates to a preparation method of a phospholipid biomimic membrane containing aquaporins. A composite membrane the surface of which is electronegative is prepared by layer-by-layer assembly, and a phospholipid vesicle solution with electropositivity, which is wrapped with aquaporins, spreads on a composite membrane by an electrostatic bonding effect so as to form a phospholipid dual-layer membrane. According to the invention, the phospholipid biomimic membrane containing aquaporins is prepared by layer-by-layer assembly, after aquaporins is added, the flux of the membrane is improved by 4-5 times compared with the original membrane, and is about 16-28L / m2h (the water flux of the original membrane is 3-5L / m2h); the reject rate of monovalence Na<+1> is improved by more than 40%; the reject rate of bivalence Mg<+2> is improved by more than 65%; and the preparation method of the phospholipid biomimic membrane containing aquaporins can be applied to different fields of water softening, wastewater treatment and the like, and has a wide application prospect.

A method for preparing a giant phospholipid vesicle by using a finger-like microelectrode relates to a preparation method for the giant phospholipid vesicle. The method for preparing the giant phospholipid vesicle by using the finger-like microelectrode is realized by the following technical scheme: firstly, sequentially carrying out ultrasonic cleaning on a finger-like mircroelectrode with absolute ethyl alcohol and distilled water, drying the finger-like mircroelectrode by blowing with nitrogen gas and treating the dried finger-like mircroelectrode through a plasma cleaning machine for later use; secondly, dissolving phospholipid into chloroform to prepare a phospholipid solution, tiling the phospholipid solution on the finger-like mircroelectrode and carrying out vacuum drying on the finger-like mircroelectrode; thirdly, assembling the dried finger-like mircroelectrode coated with phospholipid, a polyfluortetraethylene rectangular frame and cover glass to form a device, injecting a sucrose or glucose solution in the device and placing the device on a heating plate; and fourthly, respectively connecting the electrode with a signal generator, setting the voltage and the frequency and forming the giant phospholipid vesicle. The method disclosed by the invention has the advantages of small electrode area, mild reaction conditions, uniformity in size, favorable controllability of the generated giant phospholipid vesicle and the like.

The invention relates to a preparation method for a double-skin forward permeable membrane with aquaporin. The preparation method specifically comprises the following steps of: 1) preparing a base membrane; 2) preparing a layer-by-layer assembly composite membrane; 3) preparing phospholipid vesicle solution; 4) introducing aquaporin; and 5) preparing a biomimic film with aquaporin phospholipid, wherein proteoliposome with aquaporin prepared in the step 4 is spread to the surface of the composite film prepared in the step 2, and then a cross-linking agent is used to carry out cross-linking to complete the preparation. According to the preparation method, the double-skin forward permeable membrane with aquaporin is prepared by layer-by-layer assembly; the double-skin forward permeable membrane can effectively prevent solute from entering the composite membrane, thereby reducing inner concentration polarization effect, and obviously improving effective water flux of the forward permeable membrane; and the the double-skin forward permeable membrane can be applied to different fields such as water treatment, membrane separation and the like, and has a wide application prospect.

Mitochondriotropic phospholipid vesicles, i.e., mitochondriotropic liposomes, that comprise a hydrophobized amphiphilic delocalized cation, such as those comprising, e.g., a triphenylphosphonium or a quinolinium moiety, incorporated into the phospholipid membrane of the vesicles, or liposomes, are disclosed. The hydrophobized portion of the amphiphilic delocalized cation, e.g., a fatty acid or other phospholipid derivative, is embedded in the phospholipid membrane of the liposome, and the amphiphilic portion of the cation is exposed on the surface of the liposome. Mitochondriotropic liposomes constitute a mitochondria-targeted drug delivery system, permitting the transport of a high payload of therapeutic water-soluble molecules in their native (i.e., active) state specifically and exclusively to mitochondria in living mammalian cells.

Disclosed are compositions for treating blood coagulation disorders and allows for manipulation of the blood coagulation cascade. More particularly the invention, relates to compositions for altering bleeding that include a mixture of at least one blood coagulation factor in a low dose and phospholipid vesicles. The invention has a variety of important uses including controlling bleeding in a mammal that has or is suspected of having a potentially life-threatening blood coagulation disorder.

The invention discloses to a method used for simultaneous preparation of phospholipid microtubule and vesicle using point-plane electrode electric field. The method is used for solving a problem that existing method is not capable of realizing simultaneous preparation of phospholipid microtubule and vesicle. The method comprises following steps: step 1, electrode cleaning; step 2, preparation of phospholipid dry membrane; step 3, assembling of a sealed preparation apparatus; and step 4, preparation of the phospholipid microtubule and vesicle, and obtaining of the phospholipid microtubule and vesicle. Advantages of the method are that: firstly, the method is capable of realizing simultaneous preparation of the phospholipid microtubule and vesicle using point-plane electrode electric field in a same system, form of the obtained phospholipid microtubule is good, yield is high, and phospholipid microtubule size is relatively large and uniform; and secondly, the length of the obtained phospholipid microtubule ranges from 200 to 1300 mum, and diameter ranges from 1 to 2 mum; and diameter of the obtained phospholipid vesicle ranges from 15 to 120 mum. The method is used for simultaneous preparation of the phospholipid microtubule and vesicle using point-plane electrode electric field.

The invention relates to a preparation method for a drug carrier, in particular to a preparation method for an acidity-controllable drug carrier. The problems that when existing lipidosome is used as a drug carrier, the lipidosome is unstable in a human body and is prone to breaking, internal drug leaks, and consequently a side effect is caused to sensitive tissue; meanwhile, not all the lipidosome can be used as the carrier of acidity-controllable slow release drugs, and limitation exists are solved. The preparation method comprises the steps that 1, ITO surface cleaning is carried out; 2, a vesicle solution is prepared; 3, a phospholipid vesicle-poly-dopamine complex is prepared. The preparation method is used for preparing the acidity-controllable drug carrier.

The invention provides a preparation method of a lipidosome-protected nano-gold gene vector. The preparation method comprises the following steps: dissolving dimethyldioctadecylammonium bromide and dioleoyl phosphatidyl ethanolamine into an organic solvent, removing the organic solvent, dissolving into water, and performing ultrasonic treatment to obtain a phospholipid vesicle solution, wherein the molar ratio of the dimethyldioctadecylammonium bromide to the dioleoyl phosphatidyl ethanolamine is 1:10-10:1; and mixing the phospholipid vesicle solution with a compound containing gold ions, adding a reducing agent, and reacting to obtain the lipidosome-protected nano-gold gene vector. After being compounded with genes, the lipidosome-protected nano-gold gene vector is high in transfection efficiency and high in gene release speed in a specific environment; the lipidosome-protected nano-gold gene vector is high in stability in serum and is not aggregated, and a transfection process is simplified since serum in a culture solution does not need to be removed in an in-vitro transfection process; the carrying amount of DNAs (Desoxvribose Nucleic Acids) is high, the using quantity of the DNAs is reduced, and the immunogenicity of exogenous DNAs to cytotoxicity and organisms are lowered.

In an inexpensive and convenient method for immobilizing a suspension cell, a phospholipid vesicle or the like regardless of the type of cell, on the surface of a solid phase, a cell is immobilized by causing the cell to contact a support having a hydrophobic chain and a hydrophilic chain.

The present invention relates to development of efficacious pharmaceutical compositions comprising an active compound in a therapeutically effective amount encapsulated or solubilized in phospholipid vesicles.

The invention relates to the field of surface chemistry and applied chemistry, in particular to a method for modifying an air stability phospholipid membrane on a solid surface. The method comprises the following steps of: 1) preparing small single-wall phospholipid vesicle solution of poly(ethylene glycol) (PEG) modified phospholipid molecules, wherein the mole percentage of the PEG modified phospholipid molecules is no more than 5 mol percent; 2) performing standing reaction at room temperature between the small single-wall phospholipid vesicle solution obtained in the step 1) and the chitosan modified solid surface; and 3) removing unbonded phospholipid vesicle. The method can solve the problem of air instability when the phospholipid membrane is modified by the conventional method; and due to the addition of the PEG modified phospholipid, the preparation time for the small single-wall phospholipid vesicle is greatly shortened and the stability of the phospholipid vesicle is improved. The phospholipid bilayer formed by the method has the advantages of good air stability, convenient operation and good repeatability. If 2 to 3 mol percent PEG modified phospholipid molecules are added, the single phospholipid bilayer with the air stability can be modified.

The invention belongs to the technical field of preparation of phospholipid membranes and discloses a preparation method of a bionic phospholipid membrane with a controllable shape. The preparation method comprises the following steps: after mixing phospholipid and cholesterol, dissolving with an organic solvent; carrying out rotary evaporation to remove the organic solvent to obtain a phospholipid thin film; carrying out hydration and homogenization treatment on the obtained phospholipid thin membrane in sequence to obtain a vesicle solution with the grain diameter range of 50 to 150nm; afterdiluting the obtained vesicle solution by utilizing a hydration solution, enabling the vesicle solution to flow through the surface of a gold sheet, so as to enable phospholipid vesicles to be adsorbed on the surface of the gold sheet to reach saturation; then washing a vesicles adsorption layer on the surface of the gold sheet by utilizing the hydration solution, so as to obtain the bionic phospholipid membrane, wherein the hydration solution is a Tris buffering solution or a water solution which contains or does not contain metal cations. The phospholipid membranes with different shapes areprepared on the same surface through controlling types of the metal cations in the solution, and resources are saved; a preparation process has good stability and repeatability; an operation processis simple and the prepared bionic phospholipid membrane has a few of defects.

The invention relates to the field of drug carrier design, and particularly relates to an amphipathic phospholipid molecule with reducing response and application thereof in drug sustained release. According to the amphipathic phospholipid molecule, n-alkyl thiol serving as an initial raw material is firstly activated by 2,2'-dithiodipyridine, and further reacts with mercaptopropionic acid to obtain fatty acid containing a disulfide bond on delta 3 position; carboxylic acid performs an esterification reaction with 1-hexadecanoyl-sn-glycero-3-phosphocholine to obtain an amphipathic phospholipid molecule of glycerol 2-hydroxyl ester containing disulfide bond on the delta 3 position. The phospholipid molecule can be prepared by a simple organic unit reaction, and the phospholipid vesicle prepared from the phospholipid molecule has excellent drug encapsulating and controlled-release capabilities.

In an inexpensive and convenient method for immobilizing a suspension cell, a phospholipid vesicle or the like regardless of the type of cell, on the surface of a solid phase, a cell is immobilized by causing the cell to contact a support having a hydrophobic chain and a hydrophilic chain.

The invention relates to toad venom alcohol plastid and a preparation method thereof. The preparation method adopts a novel preparation technique to prepare a phospholipids vesicle vector containing ethanol with high concentration capable of reaching 20 to 50 percent. The plastid having the advantage of good stability is a novel preparation easy to industrialize.

The invention relates to a preparation method for artificial cells containing a cytoplasm-like substance and solves the problem that the interior of existing artificial cells is almost aqueous solution. The preparation method comprises the following steps: firstly, preparing cytoplasm-like sol; secondly, cleaning an ITO glass electrode; and thirdly, preparing phospholipid vesicles containing the cytoplasm-like substance, and obtaining the artificial cells containing the cytoplasm-like substance. The preparation method provided by the invention is used for preparation of the artificial cells containing the cytoplasm-like substance.

The invention discloses a method for preparing and producing a hemostatic dressing embedded with a tissue factor by taking calcium carbonate as a raw material. The method comprises the following steps: (1) expressing and purifying in bacteria to obtain the tissue factor with biological activity; (2) treating phospholipid and the tissue factor obtained in the step (1) to obtain a combination of the tissue factor and phospholipid vesicles; (3) adding the tissue factor-vesicle combination obtained in the step (2) into a calcium chloride solution, fully and uniformly mixing, adding a sodium carbonate solution, and carrying out post-treatment to obtain CaCO3 particle powder; and (4) uniformly mixing the CaCO3 particle powder obtained in the step (3) with acid powder to obtain the hemostatic dressing with the self-propelling capability. The tissue factor is a protein with biological activity and efficient blood coagulation capability, and can start a human body blood coagulation cascade exogenous pathway to stop bleeding. The prepared hemostatic dressing is in the form of powder, has self-pushing capacity, can push tissue factors to the depth of a wound, is suitable for irregular wounds which have certain depth and cannot effectively stop bleeding by a traditional dressing, and is wide in application environment. By adding the tissue factors, the hemostatic dressing can play a more active role in wound management, the passive hemostatic effect of a traditional hemostatic dressing in wound management is replaced, the biocompatibility is better, the degradability is high, and the risk of secondary damage to the wound can be reduced.

The invention provides a preparation method of a highly ordered phospholipid vesicle array, and relates to a preparation method of phospholipid vesicles. The object of the invention is to solve the problems that the phospholipid vesicles prepared by an existing large-area electrode are disordered, and the sizes of the phospholipid vesicles are not uniform. The method comprises the following steps:first, preparation and cleaning of an electrode; second, coating and drying of a phospholipid dry film; third, assembly; and fourth, performing an electric vesicle forming process to obtain the highly ordered phospholipid vesicle array. By adoption of the preparation method provided by the invention, the simple and rapid preparation of the phospholipid vesicles with highly ordered arrangement anduniform size is creatively achieved, the preparation time is short, and the preparation method is suitable for industrial production. The invention provides the preparation method of the highly ordered phospholipid vesicle array.

The invention provides a preparation method of an eukaryotic cell model, and relates to a preparation method of cell models. The problems that an existing cell model only simulates an eukaryotic cell, and research of life evolution is not satisfied are solved. The method comprises the steps that 1, a single-chamber phospholipid vesicle is prepared; 2, an artificial eukaryotic cell model is prepared; 3, PCR amplification is conducted; 4, artificial cell division is conducted. According to the preparation method of the eukaryotic cell model, an artificial eukaryotic cell of which the structure is complete, the size is uniform, and the size of a small vesicle is close to that of a human body cell nucleus is prepared, a PCR technology and a fission process are conducted in the artificial cell nucleus successfully, and the functionalization of the artificial cell nucleus is achieved. The preparation method of the eukaryotic cell model is used for preparing the eukaryotic cell model.

The invention discloses a biosensor interface constructed through a phospholipid membrane layer. The biosensor interface can effectively hinder nonspecific adsorption, introduction of a sealing agent is avoided, a bionic environment capable of protecting activity is provided for the subsequent molecular recognition process, and the requirement of a biosensor for a sensing interface is met. The biosensor interface comprises 1-decanethiol mixed with phospholipid vesicles, DPPC (dipalmitoylphosphatidylcholine) used for forming single-layer phospholipid vesicles, cholesterol used for forming a phospholipid modified layer as well as a biosensing surface, wherein the biosensing surface is a transparent solid substrate plated with a 50-nm gold membrane, a phospholipid mixture mixed with the 1-decanethiol fixes the single-layer phospholipid vesicles on the biosensing surface by the aid of sulfhydryl groups of the 1-decanethiol. The invention further provides a preparation method.

The invention discloses a method for improving cohesiveness of a high-protein nutrition bar by adding phospholipid vesicles, and belongs to the technical field of food processing. The nutrition bar comprises milk protein concentrate having a phospholipid vesicle content of 3-10% (based on the solid content of the milk protein concentrate), sorbitol, glycerol and water. The system can keep a complete structure without breaking after being pressed down in a storage process, and is stable, continuous and uniform, fine and smooth in texture and difficult to break when subjected to external force, and hardness and texture are also greatly improved. After a certain amount of phospholipid is added into the high-protein nutrition bar, the cohesiveness of the high-protein nutrition bar can be greatly improved, and the nutritional value of the high-protein nutrition bar can be increased.

The invention relates to a preparation method for a carboxylic acid molecule, specifically to a photosensitive carboxylic acid molecule, and a preparation method and application thereof. According to the invention, 5-hydroxyl-nitrobenzaldehyde is used as a starting material to prepare o-nitrobenzyl-containing fatty acid through simple unit synthesis; light-degradable-unit-containing carboxylic acid is prepared through simple standard organic unit reactions like etherification, reduction and esterification; meanwhile, the method in the invention uses cheap raw materials and has simple synthetic process, so the photosensitive carboxylic acid molecule with different structures can be prepared with low cost; and an obtained target product can reach a level of hectogram and realize the purity of more than 98% through simple crystallization. In addition, the invention also discloses the application of the photosensitive carboxylic acid molecule in drug sustained release; meanwhile, drug-loading and photocontrolled release capacities of photosensitive phospholipid vesicles synthesized from light-degradable carboxylic acid prove that the photosensitive carboxylic acid molecule has good photoresponse performance.

A Cannabis sativa-derived formulation for transmucosal and transdermal delivery is disclosed comprising Cannabis sativa-derived compounds nanoencapsulated in phospholipid-based vesicles. A method of encapsulating cannabis-derived compounds in nanosized phospholipid vesicles is also disclosed. Cannabis sativa-derived extracts have enhanced bioavailability when encapsulated in nanosized phospholipid vesicles prior to administration to a subject as compared to non-encapsulated cannabinoids. Also disclosed herein are methods of transmucosal and transdermal administration of the formulation having phospholipid vesicles with nanoencapsulated Cannabis sativa-derived substances.

The invention relates to a preparation method of a bacteria model, in particular to a preparation method of a magnetotactic bacteria model. The preparation method provided by the invention solves the problem that for the process that polymers wrap magnetic nanoparticles to build a model, the simulation is only performed in a function and not performed in a structure in the prior art. The preparation method comprises the steps of 1, preparation of phospholipid vesicle; 2, preparation of the bacteria model, namely the preparation method of completing the magnetotactic bacteria model. The invention is used for the preparation of the magnetotactic bacteria model.

The invention relates to the field of drug carrier design, and particularly relates to an amphipathic phospholipid molecule with reducing response and application thereof in drug sustained release. According to the amphipathic phospholipid molecule, n-alkyl thiol serving as an initial raw material is firstly activated by 2,2'-dithiodipyridine, and further reacts with mercaptopropionic acid to obtain fatty acid containing a disulfide bond on delta 3 position; carboxylic acid performs an esterification reaction with 1-hexadecanoyl-sn-glycero-3-phosphocholine to obtain an amphipathic phospholipid molecule of glycerol 2-hydroxyl ester containing disulfide bond on the delta 3 position. The phospholipid molecule can be prepared by a simple organic unit reaction, and the phospholipid vesicle prepared from the phospholipid molecule has excellent drug encapsulating and controlled-release capabilities.

The invention discloses a method for preparing a phase-separation giant phospholipid vesicle array by using a point-surface electrode electric field and on the basis of a micro contact stripping technique, and relates to the method for preparing the phase-separation giant phospholipid vesicle array. The invention aims to solve the problem that a phase-separation giant phospholipid vesicle array cannot be prepared at present. The method comprises the steps: 1, cleaning an electrode; 2, cleaning a polydimethyl siloxane stamp with a pattern; 3, preparing a mixed phospholipid dry film; 4, preparing an ITO electrode of a ternary mixed phospholipid dry film array; 5, assembling an airtight preparation device; and 6, performing a phase-separation giant phospholipid vesicle array formation process, to obtain the phase-separation giant phospholipid vesicle array. The diameter of phospholipid vesicles in the prepared phase-separation giant phospholipid vesicle array is 30 [mu]m to 50 [mu]m. The method for preparing the phase-separation giant phospholipid vesicle array by using the point-surface electrode electric field and on the basis of the micro contact stripping technique is obtained.