The invention discloses an environment-friendly wood floor based on waste wood. A preparation method of the environment-friendly wood floor comprises a waste wood treatment technology and an environment-friendly wood floor moulding technology, wherein the waste wood treatment technology comprises the steps of classifying, impurity removal, axial cutting, rot removal with air blades, ultrahigh temperature sterilization, axial crushing, fiber isolating, drying and storing; the environment-friendly wood floor moulding technology comprises the steps of fiber screening, low-pressure glue spraying in air, stirring, high-temperature high-pressure moulding, membrane laminating and cutting. According to the environment-friendly wood floor based on waste wood, first, the waste wood is classified, then rotten parts are removed, wood fibers are isolated so that the isolated wood fibers can have different levels, the wood fibers are sufficiently utilized, and thus secondary waste cannot be caused;furthermore, through low-pressure glue spraying in air, fibers which are completely wrapped with glue are obtained, possible defects of fiber damage on the waste wood are overcome through artificial protection, and the function of the high-strength wood floor is achieved.