37results about How to "High academic value" patented technology

The invention discloses a preparation method of traditional Chinese medicine ultrafine formula particles. The preparation method comprises the following steps that: single Chinese medicinal material or traditional Chinese medicine decoction pieces are partially ultrafine-crushed into ultrafine powder, the other parts are coarse-crushed, extracted, concentrated, dried and crushed to obtain dry extract powder, the ultrafine powder and auxiliary materials are added to obtain mixture, and the obtained mixture is mixed uniformly, palletized, granulated and subpackaged. The traditional Chinese medicine ultrafine formula particles is characterized in that the characteristics of the traditional Chinese medicine ultrafine decoction pieces of saving medicine materials and being controllable in quality and convenient in administration. Compared with the traditional Chinese medicine formula particles, the preparation method better preserves the medicinal properties of the traditional Chinese medicines, has the advantages of little used amount of auxiliary materials, convenient identification, benefit to measuring the content of index ingredients, preserves the advantages that the traditional Chinese medicine decoction piece can be added and reduced according to the disease and the traditional decoction has unique curative effect, also overcomes the defects of troublesome decoction and inconvenient administration, and has advanced technique and controllable quality. The invention also discloses a processing method of the Chinese medicinal material, which extracts the raw medicinal materials partially, carries out ultrafining treatment partially and then carries out mixing. The method can obvious improve the utilization ratio and leachability of the Chinese medicinal materials.

The invention relates to a method for quickly separating a high-purity cowherb seed flavonoid glycoside monomer from a Chinese medicinal material cowherb seed and belongs to the technical field of Chinese medicines. The method comprises the following process steps of: A, extracting raw materials, namely poaching; B, enriching by using macroporous resin; C, performing high-efficiency preparation and liquid phase separation, wherein C18 fillers are adopted, the detection wavelength is 280 nm and the mobile phase is a 30 volume percent methanol solution; and D, recovering products, namely enriching a preparation solution by using the macroporous resin, desorbing by using ethanol, concentrating and drying to obtain the cowherb seed flavonoid glycoside monomer with the purity of over 99 percent. The method is simple and quick, low in solvent consumption, high in yield, and economic and environment-friendly. A large number of products can be prepared by the method at one time. The method has high economic value and academic value.

The invention discloses a method for separating and purifying coptisine monomers, comprising the steps of extracting, concentrating, leaching, dissolving and filtering, performing preparative high performance liquid chromatography and separating, recycling products and the like. The method of the invention has simple and convenient operation, short production cycle, high separating efficiency, stable process and low cost, and can realize preparing great amount of the high-purity coptisine monomers by separation; and the obtained coptisine monomers have high purity, and can be used as comparison products in assaying.

The invention relates to a separation and purification method for a Thonningianin A monomer and belongs to the technical field of separation and purification of active components of traditional Chinese medicines. The separation and purification method comprises the specific steps of (1) extraction: cutting penthorum chinense pursh into small pieces, performing backflow extraction on the small pieces through ethanol, and then filtering the small pieces; (2) concentration: concentrating filtrate to 20 percent; (3) enrichment and elution of macroporous adsorption resin; (4) enrichment and elution of polyamide resin; (5) high-efficiency preparation and liquid chromatographic separation, wherein the flowing phase is an acetonitrile-3% phosphoric acid aqueous solution, and the detection wavelength is 254nm; (6) product recycling: filtering a Thonningianin A monomer solution to separate out crystals, and drying the crystals to obtain the Thonningianin A monomer product. The separation and purification method is easy and convenient to operate, short in production period, high in separation efficiency, stable and reliable in technology and high in reproducibility; the cost is easy to control, and the product is high in purity; the Thonningianin A monomer can be used as a standard substance for measuring the content.

The invention discloses a preparation method of polylactic acid/chitosan/carbon fiber porous supports. The preparation method comprises the steps: weighing dispersed and well-dried carbon fiber and polylactic acid solution and ultrasonically oscillating and mixing the two so that the carbon fiber is dispersed in the polylactic acid solution; then mixing the two with a certain proportion of chitosan solution evenly; pouring the evenly mixed tertiary mixture in a support die; and putting the die in a freezing drier to be frozen quickly, preserved with heat, vacuumized and dried, and finally demolding and washing, thus obtaining a polylactic acid/chitosan/carbon fiber porous support. The developed compound porous support has the advantages of simple manufacture technology, good mechanical performance, highly porous property, favorable compatibility with cells and the like, and has wide application prospect in the medical field.

The invention provides a method for preparing a specnuezhenide reference substance. The preparation method specifically comprises the following steps: (i) providing specnuezhenide alcohol extract; (ii), mixing the specnuezhenide alcohol extract and a solvent to obtain a first mixture, and performing column chromatography separation on the first mixture by virtue of an intermediate-pressure preparation column, thereby obtaining a crude specnuezhenide product; (iii) performing recrystallization on the crude specnuezhenide product by virtue of purified water; (iv) separating and drying crystals obtained in step (iii), so as to obtain the specnuezhenide reference substance. The method is stable in process, convenient to operate, high in separation efficiency, low in cost, economical and environment-friendly, and has relatively high economic value and academic value.

The invention provides a double-beam laser heating auxiliary drilling integration device and method, and belongs to the technical field of machining. The double-beam laser heating auxiliary drilling integration device comprises a continuous laser generation system and a pulse laser generation system, wherein the continuous laser generation system comprises a continuous laser generator and a continuous laser focusing head; a manipulator clamps the continuous laser focusing head to move so as to adjust the irradiation direction of a continuous laser beam, the size of a laser spot, the laser movement speed and the distance between the center of the laser spot and the surface of a workpiece; the pulse laser generation system comprises a pulse laser generator and a collimation system which areconnected with each other; and the pulse laser beam irradiates the surface of the workpiece from the interior of a drill bit along a drill tip through the collimation system. Compared with single-beamlaser heating, the double-beam laser heating auxiliary drilling integration device and method have higher machining efficiency and lower surface damage, drilling machining of transparent materials difficult to machine is realized, and the cost is reduced.

The invention provides a water landscape design method enabling flow characteristics of tidal floods rarely happening to reappear scientifically. Actual measurement terrain, geological data and hydrologic data of a natural river course are collected; the range of model, the number of section boards and adopted materials are determined according to a similarity criterion; a lofting section is selected from an actual measurement topographic map, and a model section board the same as a natural riverbed in form is manufactured; a control wire of the model is determined, a model side wall line is designated, a horizontal control network and a vertical control network are made, a bench mark is set, and the model is installed according to a river course topographic map; actual measurement runoff flow hydrographs and river mouth actual measurement tide tide-level hydrographs of dry season typical days and flood typical days of a natural river hydrometric station are verified; flow rates of corresponding sections of a natural riverbed are verified; erosion and deposition verification is carried out on the actual measurement topographic map of the natural river course and the model before and after channel forming discharge occurs; a water circulation system is established, so that water landscapes designed according to the method have extremely high academic value and added economic value.

The invention discloses a method for separating and purifying dehydroevodiamine from fructus evodiae which is a rutaceous plant. The method comprises the following steps: soaking a fructus evodiae fruit by using a petroleum ether to degrease, then extracting with 2% HCl as a solvent through a reflux extraction method, collecting an extracting solution, then carrying out vacuum concentration or freeze-drying to obtain an extractum A, dissolving the extractum A with methyl alcohol, filtering, carrying out vacuum concentration or freeze-drying on a filtrate to obtain an extractum B, taking polyamide resin as a filler to elute the extractum B with water-ethyl alcohol, fractionally collecting a semi-finished product, conducting vacuum concentration or freeze-drying to obtain a solid semi-finished product, taking ethyl alcohol-acetone as a solvent to recrystallize the solid semi-finished product to obtain a dehydroevodiamine powder crystal with the purity more than 98.5%. The yield of the dehydroevodiamine prepared through the method is high, the cost is low, the operation is simple, and the method is environmentally friendly, and is suitable for large-scale industrial production.

A framing off-axis X-ray holographic apparatus has the characteristics that it includes quasi-monochromatic X ray source, grating, the first reflector, the second reflector, X-ray wave zone plate and recording medium, a grating is installed on the output optical path of quasi-monochromatic X-ray source, dividing the X ray to be A and B light beam, the A bight beam is to be referential beam after being reflected by the first reflector and the B light beam is to be subject beam through a subject to be measured after being reflected by second reflector, then the two beams meet together and are recorded by medium through X ray wave zone plate.

The invention discloses a preparation method of graphene. The lignin-like substance is used as a raw material, tetrahydrofuran is used as a modifier, ferric nitrate nonahydrate is used as a catalyst,lignin is subjected to water bath treatment by the modifier and the ferric nitrate nonahydrate and then calcined to obtain graphene; the lignin-like substance is straw fermentation biogas residue or straw acid hydrolysis residue, the preparation method of the graphene disclosed by the invention has the advantages that the process is simple, the conversion from low-added-value biomass waste to high-added-value product graphene is realized, and the important significance is realized.

The invention discloses a high-efficiency separation method of high-purity wild baicalin, comprising the following steps of: (1) dissolving raw materials, wherein DMF (Dimethyl Formamide) is used as solvent dissolution-assistant raw materials; and (2) using methanol-DMF-water as a mobile phase, separating by adopting a high-efficiency preparation liquid-phase chromatographic column, recovering the solvents and drying to obtain a baicalin product with the purity greater than 99%. The method has the advantages of simplicity and rapidity, less solvent consumption, high yield, economy and environmental protection, can be used for preparing a great deal of products once and has higher economic value and academic value.

The invention provides a multiple PCR (Polymerase Chain Reaction) primer composition, a reagent and a multiple PCR-breaking control system used for TERT (Telomerase Reverse Transcriptase) gene whole exome next-generation sequencing. The multiple PCR primer composition used for the TERT gene whole exome next-generation sequencing comprises primers disclosed from SEQ ID NO: 01 to SEQ ID NO: 32. Themultiple PCR primer composition, the reagent and the multiple PCR-breaking control system for the TERT gene whole exome next-generation sequencing solves the following existing technical problems that1: since the point mutation of the BRAF gene is mainly detected in the prior art, a detection range is limited; 2: real-time fluorescent quantitation PCR (Polymerase Chain Reaction) detection and a gene chip only can detect known sites; 3: a Sanger sequencing checkout length is smaller than a range of 800 bp; 4: low-frequency mutation can not be detected; and 5: when a plurality of sites are detected, operation is complex, and cost is high.

The invention provides a multiplex PCR primer composition, a reagent and a control system for next-generation sequencing of whole exons of TERT gene. The multiplex PCR primer composition for the second-generation sequencing of the whole exons of the TERT gene comprises primers shown as SEQ ID NO: 01 to SEQ ID NO: 50. The multiplex PCR primer composition, the reagent and the control system for thesecond-generation sequencing of the whole exons of the TERT gene provided by the invention solve the following existing technical problems: 1, the point mutation of a TERT gene promoter is mainly detected in the prior art, and the detection range is limited; 2, real-time fluorescent quantitative PCR detection is carried out, and the gene chip can only detect known sites; 3, the Sanger sequencing test length is in the range of less than 800bp; 4, when multiple sites are detected, the operation is tedious, and the cost is high; and 5, low-frequency mutation cannot be detected.

The invention discloses a chromatographic analysis method for thoroughly solving the peak shape of a dehydroevodiamine chromatographic peak, which adopts reversed-phase high performance liquid chromatography, takes hydrochloric acid as an additive for the first time, adjusts the pH value of a mobile phase to be 1-2, remarkably improves the problem of trailing of the dehydroevodiamine chromatographic peak, has simple analysis conditions, is convenient to operate, and is suitable for industrial production. The chromatographic mobile phase additive is economical and environment-friendly; symmetry factors of chromatographic peaks meet requirements of Chinese pharmacopoeia, and chromatographic analysis conditions can be used for measuring the content of dehydroevodiamine in medicinal materials, Chinese patent medicines and biological samples of fructus evodiae.

The invention discloses a method for analyzing the stability of a crack-containing bentonite slope reinforced by a pre-stressed anchor cable, and provides a rotation-translation combined mechanism to evaluate the stability of the crack-containing bentonite slope, a translation mechanism is adopted to simulate a crack sliding surface, and a rotation mechanism is adopted to simulate an arc sliding surface. Aiming at the characteristic that the unit weight of bentonite is in nonlinear change along the depth of a soil layer, a sliding soil body is dispersed into a series of soil units, the power made by the gravity of the bentonite is calculated by adopting a layered summation method and a rotational volume integration method, and the total power made by the gravity is obtained by accumulation. And obtaining a bentonite slope safety coefficient expression according to a volume weight increase principle, and searching a slope minimum safety coefficient based on an enumeration method to obtain a corresponding critical sliding surface. According to the method, the bentonite strength theory and the limit analysis method can be effectively combined, the slope suction effect and the anchor cable action mechanism are reasonably explained, and the method has important academic value and practical significance for guiding slope design, construction and reinforcement.