The utility model belongs to the field of sample storage, and discloses a detection sample separated storage device which comprises a box body, the sample cylinder comprises a bottom plate, a first side plate and a second side plate, the first side plate is fixed on the bottom plate, the second side plate is rotatably mounted on the bottom plate, the projection of the first side plate on the horizontal plane is semicircular, the projection of the second side plate on the horizontal plane is arc-shaped, and the outer wall of the second side plate abuts against the inner wall of the first side plate; the cover plate is detachably connected with the first side plate and the second side plate; and the box cover is mounted on the box body. The side wall of the sample cylinder is provided with the fixed first side plate and the movable second side plate, and the second side plate can rotate to open the sealed space enclosed by the second side plate and the first side plate, so that a user can conveniently take out a sample bag, a sample tube and the like in the sample cylinder, the taking of a sample is facilitated, and the use is convenient. And the sealing can be recovered in a rotating manner, so that the sample
barrel can limit the position of the sample bag.