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Fast teething and reproduction method for Mafeng tree

A Jatropha curcas, fast technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of loss of good traits, high variation rate of differentiated seedlings, Jatropha curcas is susceptible to seasonal and geographical restrictions, etc. Achieve the effects of avoiding the maintenance of excellent traits, fast propagation speed and high number of buds

Inactive Publication Date: 2008-08-06
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are following problems in these two kinds of methods: (1) realize the slow speed of Jatropha curcas individual reproduction by natural growth
(2) The individual reproduction of Jatropha curcas is easily restricted by season and region by natural growth
(3) Natural reproduction is not conducive to the maintenance of good traits
Although the method of callus induction and differentiation into seedlings improves the individual reproduction speed compared with the traditional propagation and cultivation method, and avoids the individual reproduction being easily affected by seasonal and geographical restrictions, but the increased individual reproduction speed is not ideal enough, and this kind of differentiated seedlings vary. The rate is high, and some good traits may be lost during the differentiation process
In addition, since the process of callus induction and differentiation into shoots is induced by high concentration of cytokinins, the obtained tissue culture plantlets have the problem of difficulty in rooting, and the tissue culture plantlets with poor root system development will be difficult once transplanted in the soil. Will die soon, cause it can only grow on the medium, thus can not provide excellent rooting seedlings for Jatropha afforestation

Method used

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  • Fast teething and reproduction method for Mafeng tree
  • Fast teething and reproduction method for Mafeng tree
  • Fast teething and reproduction method for Mafeng tree

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1～6、 comparative example 1

[0024] This group of embodiment and comparative example all adopt the Jatropha curcas stem tip of natural growth to carry out rapid propagation.

[0025] Sterilization process is carried out according to each embodiment and comparative example inoculation quantity, intercepts the Jatropha curcas stem tip 1~2cm of natural growth respectively, puts into 70% alcohol and shakes 1min, discards alcohol, then uses 0.1% HgCl 2 The solution was sterilized for 7 minutes, and the HgCl was discarded 2 , and then rinsed 5 times with sterile water to thoroughly wash away residual HgCl 2 .

[0026] Preparation of rapid propagation medium In MS medium numbered from (1) to (9), add cytokinin 6-benzyladenine (BA), auxin indole and butyric acid (IBA) and silver nitrate, and adjust its pH to 5.8.

[0027] Rapid Propagation Absorb the water on the surface of the shoot tip after sterilization, cut off the excess part, keep only the tender part of the tip, and then transfer it into the rapid prop...

Embodiment 9～17

[0032] The examples of this group all adopt the aseptic vaccine that is cultivated by hormone stimulation to carry out rapid propagation.

[0033] Sterilize the quantity inoculated according to each embodiment, peel off the seed coat of Jatropha curcas seeds, take out the embryo therein, put into 70% alcohol and shake for 1min, discard the alcohol, then use 0.1% HgCl 2 The solution was sterilized for 7 minutes, and the HgCl was discarded 2 , and then rinsed 3 times with sterile water to thoroughly wash away residual HgCl 2 , add sterile water to soak for 48 hours, then pour out the sterile water. Operate again according to the above sterilization process.

[0034] Cultivate aseptic seedlings to absorb the water on the surface of the seed kernel after sterilization, cut the seed kernel (endosperm) along the longitudinal axis with a blade, and peel off two white cotyledons (the bases of the two cotyledons contain the seed embryo), and connect the cotyledons together. Embryos ...

Embodiment 18

[0041] The germs of the sterilized seedlings that germinated in the sterile seedlings that were sterilized and cultivated in the procedures described in Examples 9 to 17 were excised, and inoculated with 20 inoculations made of MS+2.0mg / L zeatin+0.05mg / L indole Acetic acid (IAA)+5mg / L AgNO 3 In the composed rapid propagation medium, the pH value of the medium is 4.8, and after 25 days of culture under the conditions of culture temperature 30°C, light intensity 2000lx, and light time 8h / d, the average number of axillary buds induced is 2.70.

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Abstract

A method for fast reproducing the chit of barbadosnut tree includes such steps as providing the bud of barbadosnut tree, sterilizing, and culturing in the fast-reproduction culture medium at 25-35 deg.C for 25-35 days while light radiating by 1500-3000 lx for 8-15 hr per day. Two methods for fast reproducing the rooted sapling of barbadosnut tree are also disclosed, which features that the aseptic nick of said chit is immersed in the aseptic auxin and then cultured in MS or 1 / 2 MS culture medium, or directly cultured in the MS or 1 / 2 MS culture medium containing activated carbon.

Description

1. Technical field [0001] The invention belongs to the technical field of rapid plant propagation, and in particular relates to a method for rapid propagation of Jatropha curcas young shoots and rooted seedlings. 2. Background technology [0002] Jatropha curcas L. is a deciduous shrub or small tree belonging to the genus Jatropha of the Euphorbiaceae family. It is native to tropical America and distributed in tropical and subtropical regions of the world. Cultivated or semi-wild in my country in Taiwan, Guangdong, Guangxi, Sichuan, Guizhou, Hainan and Yunnan. The comprehensive utilization value of Jatropha curcas is high, and its leaves can treat rheumatism, sexually transmitted diseases, angina pectoris; the branch milk has a good curative effect on warts, skin wounds, etc.; the branch extract has anti-HIV virus effect; The seeds can be used as a laxative; the toxic protein extracted from the seeds of Jatropha curcas has a strong inhibitory effect on tumor cells; the seed...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01H3/00C12N5/04
Inventor 李化唐琳陈放
Owner SICHUAN UNIV
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