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Alanine aminopherase monoclonal antibody and use thereof

An alanine amino, monoclonal antibody technology, applied in anti-enzyme immunoglobulin, biochemical equipment and methods, instruments, etc., can solve the problems of inconvenience to carry, difficult to achieve rapid detection, long reaction time, etc.

Inactive Publication Date: 2012-05-30
天津市血液中心
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  • Abstract
  • Description
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Problems solved by technology

[0007] The present invention is to solve the traditional enzymatic chemical method to detect ALT, the reaction time is long, special detection equipment is required, it is inconvenient to carry, the enzyme reagent is unstable, and it is difficult to realize fast In order to establish an ALT immunological analysis method with the advantages of simplicity, rapidity and specificity, a kind of alanine aminotransferase monoclonal antibody (monoclonal antibody, McAb) and its application are provided

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  • Alanine aminopherase monoclonal antibody and use thereof
  • Alanine aminopherase monoclonal antibody and use thereof
  • Alanine aminopherase monoclonal antibody and use thereof

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Embodiment Construction

[0021] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments.

[0022] 1. Materials and methods

[0023] 1 Cells, Animals and Reagents

[0024] SPF-grade BALB / C mice were purchased from the Experimental Animal Center of the Institute of Hematology, Chinese Academy of Medical Sciences, and the mouse myeloma cell line (Sp2 / 0) was introduced from the Basic Medical Cell Center of Peking Union Medical College, Chinese Academy of Medical Sciences. Purine (Hypoxanthine, H), aminopterin (Aminopterin, A), thymidine (Thymidine, T), pristane (pristane), Freund's adjuvant, polyethylene glycol 4000 (PEG 400) were purchased from Sigma The company, fetal bovine serum and DMEM medium were purchased from Invitrogen Company, the monoclonal antibody subclass identification reagent was the product of SouthernBiotech Company, and the horseradish peroxidase (HRP)-labeled goat anti-mouse IgG was the product of Beijing Zhongshan Jinqiao...

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Abstract

The invention discloses an alanine aminotransferase monoclonal antibody and an application, belonging to the medicine product containing antibody or the preparation of monoclonal antibody. The invention has the specificity of anti human alanine aminotransferase, which is generatd by hybridoma cell whose preservation number is CGMCC No2232. The invention uses purified ALT antigen to immunize BALB / C mice, fuses spleen cells and SP2 / 0 myeloma cells, uses indirect ELISA to screen hybridoma cell, grafts in mice abdominal, collects and purifies McAb ascetic fluid, to obtain anti human ALT specific ALT monoclonal antibody. The inventive product can be used to check alanine aminotransferase of serum. The product an specifically recognize ALT in serum without cross reaction with other proteins in serum, but with better infinity, thereby providing necessary condition for simple, quick and specific ALT immunity analysis.

Description

technical field [0001] The invention relates to the preparation of pharmaceutical preparations containing antibodies, or monoclonal antibodies, in particular to an alanine aminotransferase monoclonal antibody and its application. Background technique [0002] With the widespread development of social voluntary blood donation, practice has proved that the failure of alanine aminotransferase (Alanine Transaminase, ALT) detection is the main reason for the scrapping of blood in current blood stations. [0003] ALT is one of the important enzymes that reflect liver cell damage in the human body. Detection of serum ALT helps to identify transfusion-associated hepatitis virus carriers, and can also compensate for the undetected and infected "window" of certain hepatitis virus markers to a certain extent. "Period" defects, thereby reducing blood scrapping at blood banks due to unqualified ALT. The screening of ALT before blood donation by the staff of street blood collection vehic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/40C12N5/18G01N33/577
Inventor 袁玉华樊晶李玉艳
Owner 天津市血液中心
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