Calixarene crown ether bonded silica stationary phase and preparation method and application thereof

Abstract

The invention discloses a calixarene crown ether bonded silica stationary phase. Gamma-(2,3-epoxypropoxy)propyltrimethoxy silane is used as a coupling agent which reacts with silica under the protection of catalyst and inert gas to prepare gamma-glycidoxy propyl-bonded silica; and the gamma-glycidoxy propyl-bonded silica reacts with p-tert-butylcalix[4]-1,2-crown-4 sodium phenolate in strong alkaline conditions and under the protection of catalyst and inert gas to obtain the calixarene crown ether bonded silica gel stationary phase. Due to large bonding quantity, stable performance and better separation selectivity, the stationary phase disclosed by the invention not only has the traditional ODS (octadecylsilane bonded silica) reversed-phase chromatography performance, but also can provide sites for various actions such as complexing action, hydrogen bonding and the like; and the stationary phase has remarkable advantages in separating some specific substances which are difficult to separate (such as polycyclic aromatic hydrocarbon, phenol, phenylenediamine, phenylamine and benzoic acid compounds).

Description

technical field [0001] The invention relates to separation technology, in particular to a calixarene crown ether bonded silica gel stationary phase, and also relates to a preparation method and application of the stationary phase. Background technique [0002] With the development of separation science, liquid chromatography, especially high performance liquid chromatography, is widely used in petroleum, chemical industry, environmental protection, food, medicine, legal inspection, In clinical and natural product analysis, it plays a very important role and becomes an important technique for separating complex samples. With the development of science and technology, the separation system is becoming more and more complex, especially the separation of positional isomers and optical isomers with similar structures and properties, as well as the separation and analysis of actual samples put forward higher requirements for chromatographic techniques, especially chromatographic s...

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