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Bacillus subtilis and use thereof in prevention and control of fungus disease

A technology for bacillus subtilis and fungal diseases, applied in the direction of pest control, application, tobacco, etc., to achieve broad application prospects, strong inhibitory effect, and good disease prevention effect

Inactive Publication Date: 2012-09-05
TOBACCO RES INST CHIN AGRI SCI ACAD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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After searching, there is no literature report related to the subject of the present invention in all applied patent documents

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  • Bacillus subtilis and use thereof in prevention and control of fungus disease
  • Bacillus subtilis and use thereof in prevention and control of fungus disease
  • Bacillus subtilis and use thereof in prevention and control of fungus disease

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The inhibitory effect of embodiment 1 bacterial strain on the growth of tobacco and vegetable fungal disease pathogen

[0028] The antagonism of the strain Tpb55 to tobacco and vegetable fungal diseases was determined by the filter paper method: take 20 μl of the fermentation broth on a sterilized filter paper, dry it and move it to the center of the oat medium plate, and insert the pathogenic bacteria cakes equidistantly around the filter paper (Φ0.5cm), cultivated at 28°C for 3-4 days, and measured the size of the inhibition zone. Each target bacteria was repeated 3 times, and the results were averaged.

[0029]Oat agar medium: 30g oatmeal, 20g agar, 1000mL water. Add 1000mL of water to the oatmeal, heat it on a boiling water bath for 1h, add water to make up 1000mL after filtering through gauze, add agar gel to melt, then subpackage and sterilize (121°C, 20min).

[0030] The results are shown in Table 1. It can be seen that bacterial strain Tpb55 is effective again...

Embodiment 2

[0033] Inhibitory effect of embodiment 2 bacterial strains on tobacco red spot pathogen spore germination

[0034] With the A.alternata spores treated with the crude extract of Tpb55, the germination rate of the control spores reached 60% after 6h, but no germination was seen in the treated spores, and more than 90% of the control spores germinated after 12h ( Figure 6 Shown), while 80% of the treated spores did not germinate and became deformed. After the crude extract was treated for 24 hours, the vicinity of the germ tube began to expand, forming a bubble-like protrusion ( Figure 4 Shown), and the contents began to leak, until all digested ( Figure 5 shown).

Embodiment 3

[0035] The growth-promoting action of embodiment 3 bacterial strains to tobacco and vegetables

[0036] Prepare Tpb55 strain fermentation broth (31°C, 180r / m72h). Healthy and full tobacco, pepper, cucumber, eggplant, and tomato seeds were fermented with Tpb55 strain (with a bacterial content of about 10 8 cfu / mL) 50-fold dilution solution soaked for 12 hours, sow the seeds in sterilized earthen pots (160×220mm), take medium and sterile water as the control, treat 3 pots for each plant, and sow 15 seeds in each pot seed. After the 4th true leaf of the above-mentioned plants is fully unfolded, use 30mL of Tpb55 strain fermentation broth (with a bacterial content of about 10 8 cfu / mL) 50-fold dilution to irrigate the roots, and the corresponding amount of culture medium and sterile water were irrigated respectively in the control group. After 10 days, the net photosynthetic rate (Pn) of the plants was measured using a portable photosynthetic measuring instrument (Li-6400, Li-C...

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Abstract

The invention discloses Bacillus subtilis, which is named Bacillus subtilis. The Bacillus subtilis was collected in China General Microbiological Culture Collection Center on January 15th, 2009, with a collection number of CGMCC No.2843. The Bacillus subtilis strain disclosed by the invention is screened from tobacco leaf habitats. Experiments prove that the fermentation liquor of the strain has a strong inhibition effect on fungal pathogens such as Alternaria alternate and pepper anthracnose and demonstrates high prevention effect on Alternaria alternate, tobacco black shank, pepper anthracnose, tomato wilt, phytophthora melonis Katsura, verticillium dahlia and the like and has high growth promoting effects on tobacco, pepper, cucumber, tomato and eggplant. Therefore, the Bacillus subtilis disclosed by the invention can be used for preparing biological bactericides for preventing and controlling fungi in tobacco and vegetables and can be used for preparing growth promoting agents forpromoting the growth of tobacco or vegetables.

Description

technical field [0001] The invention relates to a bacillus subtilis and its application in the prevention and treatment of tobacco or vegetable fungal diseases and the promotion of tobacco or vegetable growth, belonging to the field of biological control. Background technique [0002] For a long time, chemical control of plant diseases has been the main method, but there are few types of fungicides and pesticides with high efficiency and low toxicity, the control effect is easily affected by environmental conditions, and it is easy to cause environmental pollution and drug resistance. Biological control has the advantages of being safe to the environment, ecology, and human health. It has played an increasing role in integrated pest management and is considered an important means to achieve sustainable agricultural development. As people pay attention to environmental safety and health, the quality requirements for tobacco (smoking food) and vegetable products are getting hi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00A01P21/00A24B15/20C12R1/125
Inventor 张成省孔凡玉张玉芹陈雪王静冯超关小红高加明罗定棋
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
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