Antrodia cinnamomea pimelie kelone compound for inhibiting growth of oral cancer tumor cells

A tumor cell, cyclohexenone technology, applied in antitumor drugs, medical preparations containing active ingredients, drug combinations, etc., can solve unpublished specific problems and other issues

A tumor cell, cyclohexenone technology, applied in antitumor drugs, medical preparations containing active ingredients, drug combinations, etc., can solve unpublished specific problems and other issues

CN102232944BActive Publication Date: 2013-03-13GOLDEN BIOTECH
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  • Antrodia cinnamomea pimelie kelone compound for inhibiting growth of oral cancer tumor cells
  • Antrodia cinnamomea pimelie kelone compound for inhibiting growth of oral cancer tumor cells
  • Antrodia cinnamomea pimelie kelone compound for inhibiting growth of oral cancer tumor cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 4-hydroxy-2,3-dimethoxy-6-methyl-5(3,7,11-trimethyl-2,6,10-dodecatriene)-2-cyclohexenone Separate

[0022] Put about 100 grams of Antrodia cinnamomea mycelium, fruiting bodies or a mixture of the two into a triangular Erlenmeyer flask, add an appropriate ratio of water and alcohol (70%-100% alcohol aqueous solution), where the alcohol is more Preferably, ethanol is stirred and extracted at 20-25°C for at least 1 hour, then filtered with filter paper and 0.45μm filter membrane, and the filtrate is collected to obtain the Antrodia camphorata extract.

[0023] Analyze the collected Antrodia camphorata extract using High Performance Liquid Chromatography (High Performance Liquid Chromatography) and RP18 chromatography tube (column), and use methanol (A) and 0.1% to 0.5% acetic acid aqueous solution (B ) As a mobile phase (the ratio of the solution is: 0-10 minutes, the proportion of B is 95%-20%; 10-20 minutes, the proportion of B is 20%-10%; 20-35 minutes, the proportion of B ...

Embodiment 2

[0026] In vitro anti-oral cancer cell activity test

[0027] In order to further test the inhibitory effect of the compound found in Example 1 on tumor cells, this example will be based on the National Cancer Institute (NCI) anti-tumor drug screening model. First, take the isolated in Example 1 4-hydroxy-2,3-dimethoxy-6-methyl-5(3,7,11-trimethyl-2,6,10-dodecatriene)-2-cyclohexenone compound , Added to the culture medium containing human oral cancer tumor cells SCC-25 to test the tumor cell viability. Among them, the cell viability test can be analyzed by the well-known MTT analysis method, and the oral cancer tumor cell SCC-25 is a human squamous cell carcinoma cell line, which belongs to the squamous cells of human tongue cancer.

[0028] MTT analysis is a common analysis method used to analyze cell proliferation, percent of viable cells, and cytotoxicity. Among them, MTT (3-[4,5-dimethylthiazol-2-yl]2,5-diphenyltetrazolium bromide) is a yellow dye, which can be absorbed by livi...

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Abstract

The invention relates to a new use of a compound, and a medical composition for inhibiting growth of oral cancer tumor cells. A antrodia cinnamomea extract is subjected to separating and purifying to prepare 4-hydroxy-2,3-dimethoxy-6-methy-5(3,7,11-trimethyl-dodeca-2,6,10-trienyl)-cyclohex-2-enone. The pimelie kelone compound can be provided for inhibiting the growth of the oral cancer tumor cells. Meanwhile, the pimelie kelone compound can be provided for preparing the medical composition for inhibiting the growth of the oral cancer tumor cells.

Description

Technical field [0001] The present invention relates to a new application of a compound, in particular to a use of a compound isolated and purified from an Antrodiacinnamomea extract to inhibit the growth of oral cancer tumor cells. Background technique [0002] The oral cavity is an important portal for the human body to carry out basic life functions such as breathing, eating, and language expression. Oral cavity cancer occurs in the lips, buccal mucosa, lower gums, upper gums, posterior molars, oral cavity, and hard Cancerous lesions in the oral cavity such as the jaw or tongue. Oral cancer is a common cancer pathology for Chinese people. The carcinogenic factors may be caused by chewing betel nuts, eating spicy food, or addicted to drinking and smoking, and the prevalence and mortality of oral cancer are increasing year by year. The prone age group has dropped to 40-50 years old, and has been younger, 90% of which are men. Most oral cancers are tumors formed by the deterior...

Claims

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Application Information

Patent Timeline
13 Mar 2013
Publication
CN102232944B
IPC
A61K31/122; A61P35/00
Inventors
刘胜勇; 温武哲