Antrodia cinnamomea pimelie kelone compound for inhibiting growth of oral cancer tumor cells
A tumor cell, cyclohexenone technology, applied in antitumor drugs, medical preparations containing active ingredients, drug combinations, etc., can solve unpublished specific problems and other issues
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Embodiment 1
[0021] 4-hydroxy-2,3-dimethoxy-6-methyl-5(3,7,11-trimethyl-2,6,10-dodecatriene)-2-cyclohexenone Separate
[0022] Put about 100 grams of Antrodia cinnamomea mycelium, fruiting bodies or a mixture of the two into a triangular Erlenmeyer flask, add an appropriate ratio of water and alcohol (70%-100% alcohol aqueous solution), where the alcohol is more Preferably, ethanol is stirred and extracted at 20-25°C for at least 1 hour, then filtered with filter paper and 0.45μm filter membrane, and the filtrate is collected to obtain the Antrodia camphorata extract.
[0023] Analyze the collected Antrodia camphorata extract using High Performance Liquid Chromatography (High Performance Liquid Chromatography) and RP18 chromatography tube (column), and use methanol (A) and 0.1% to 0.5% acetic acid aqueous solution (B ) As a mobile phase (the ratio of the solution is: 0-10 minutes, the proportion of B is 95%-20%; 10-20 minutes, the proportion of B is 20%-10%; 20-35 minutes, the proportion of B ...
Embodiment 2
[0026] In vitro anti-oral cancer cell activity test
[0027] In order to further test the inhibitory effect of the compound found in Example 1 on tumor cells, this example will be based on the National Cancer Institute (NCI) anti-tumor drug screening model. First, take the isolated in Example 1 4-hydroxy-2,3-dimethoxy-6-methyl-5(3,7,11-trimethyl-2,6,10-dodecatriene)-2-cyclohexenone compound , Added to the culture medium containing human oral cancer tumor cells SCC-25 to test the tumor cell viability. Among them, the cell viability test can be analyzed by the well-known MTT analysis method, and the oral cancer tumor cell SCC-25 is a human squamous cell carcinoma cell line, which belongs to the squamous cells of human tongue cancer.
[0028] MTT analysis is a common analysis method used to analyze cell proliferation, percent of viable cells, and cytotoxicity. Among them, MTT (3-[4,5-dimethylthiazol-2-yl]2,5-diphenyltetrazolium bromide) is a yellow dye, which can be absorbed by livi...
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