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Kit for detecting hereditary hearing loss

A kind of hereditary deafness, kit technology, applied in the direction of microbial determination/inspection, DNA/RNA fragment, recombinant DNA technology, etc.

Active Publication Date: 2014-07-02
BOAO BIOLOGICAL CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The main obstacle to achieve this goal is that since the hybridization reaction between the labeled target nucleic acid and the oligonucleotide probe immobilized on the surface of the chip is the key factor affecting the detection of the chip, only one strand of double-stranded DNA can be combined with the immobilized oligonucleotide probe on the surface of the chip. The probe on the surface of the chip performs a hybridization reaction, and the other strand acts as an interference factor for the hybridization reaction due to competition

Method used

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  • Kit for detecting hereditary hearing loss
  • Kit for detecting hereditary hearing loss
  • Kit for detecting hereditary hearing loss

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Experimental program
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Embodiment 1

[0217] Embodiment 1, application kit of the present invention detects hereditary deafness

[0218] Hereinafter, the present invention will be described in detail by taking the kit for detecting 9 SNPs / mutations related to hereditary deafness as an example.

[0219] 1. Microarray chip

[0220]The universal label array is a matrix composed of 18 label probes, which can perform hybridization reactions with multiple PCR products. In addition, there are positive controls (QC) and negative controls (BC) for quality control of microarray production. Quality control microarray Positive control (PC) and negative control (NC) of hybridization process, and positive control (MC) of streptavidin coupled with biotinylated target polynucleotide on the surface of quality control particles. Positive control (QC) is an oligonucleotide probe labeled with HEX fluorescence at one end and amino-modified at the other end, which is used to monitor the effect of microarray spotting and immobilization...

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Abstract

The invention brings forward a kit for detecting hereditary hearing loss. The kit provided by the invention comprises a primer combination and a probe combination of nine hereditary hearing loss polymorphic sites on GJB2(Cx26) gene, SLC26A4(PDS) gene and 12S rRNA(MTRNR1) gene, and can be used to accurately determine the wild type, homozygous mutation or heterzygosity of related sites and realize molecular diagnosis of hearing loss. With the application of the kit provided by the invention, detection can be carried out by cheap equipment or macroscopic observation. The primer combination, reagent panel and kit provided by the invention can be used for the screening of hereditary hearing loss to realize early detection, early prevention and prenatal guidance. The invention is of great commercial value and social significance.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a kit for detecting hereditary deafness. Background technique [0002] Deafness is the most common cause of affecting human health and causing human disability. Clinically, 60% of deafness is caused by genetic factors. In 2006, my country's second sample survey of disabled people showed that there were 26.7 million people with hearing disabilities nationwide. Among the 20 million newborns in the country every year, more than 2 out of every 1,000 newborns are children with severe hearing impairment, and about half of them are hereditary deafness. In addition, among the large number of patients with delayed hearing loss, there are also many patients Has its own genetic defect. [0003] Hereditary deafness is passed from parents to their children through inheritance or atavism. Regardless of whether one or both of the parents are deaf patients or healthy carriers, the deafness...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11
Inventor 张冠斌项光新邢婉丽程京
Owner BOAO BIOLOGICAL CO LTD
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