A kind of cic reconstituting solvent for immune complex buffer dissociating agent
A technology of immune complex and reconstituted solvent, applied in the field of medical detection, can solve the problems of specificity, good sensitivity and good repeatability, etc.
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Embodiment 1
[0065] An immune complex buffer dissociation agent, for every 150 μl of pleural and ascites samples to be tested, add the immune complex buffer dissociation agent with the following components: 135 μl of CIC separation agent, 290 μl of CIC detergent, 5 μl of CIC reconstitution solvent, 65 μl CIC Antigen Buffer Dissociation Reagent and 65 μl CIC Antigen Buffer Dissociation Neutralizer.
[0066] Wherein, every 1 L of CIC separation agent includes the following components: 5.2 g of borax, 4.1 g of boric acid, 70 g of polyethylene glycol, 6.2 g of sodium chloride, 100 μl of liquid biological preservative, and distilled water as the balance.
[0067] Each 1 L of CIC detergent includes the following components: 5.2 g of borax, 4.1 g of boric acid, 35 g of polyethylene glycol, 8.0 g of sodium chloride, 100 μl of liquid biological preservative, and distilled water as the balance.
[0068] Each 1 L of CIC reconstitution solvent includes the following components: 8.0 g of sodium chlorid...
Embodiment 2
[0080] An immune complex buffer dissociation agent, for every 150 μl of cerebrospinal fluid sample to be tested, add the immune complex buffer dissociation agent with the following components, 165 μl of CIC separation agent, 315 μl of CIC detergent, 16 μl of CIC reconstitution solvent, 76 μl of CIC Antigen Buffer Dissociation Agent and 76 μl of CIC Antigen Buffer Dissociation Neutralizer.
[0081] Wherein, every 1L of CIC separation agent includes the following components: 7.7g of borax, 6.1g of boric acid, 90g of polyethylene glycol, 9.2g of sodium chloride, 500μl of liquid biological preservative, and the balance is distilled water.
[0082] Each 1L of CIC detergent includes the following components: 7.7g of borax, 6.1g of boric acid, 45g of polyethylene glycol, 11.7g of sodium chloride, 500μl of liquid biological preservative, and the balance is distilled water.
[0083] Each 1 L of CIC reconstitution solvent includes the following components: 9.5 g of sodium chloride, 0.03...
Embodiment 3
[0095]An immune complex buffer dissociation agent, for every 150 μl of the sample to be tested, add an immune complex buffer dissociation agent with the following components: 150 μl of CIC separation agent, 300 μl of CIC detergent, 10 μl of CIC reconstitution solvent, and 70 μl of CIC Antigen Buffer Dissociation Agent and 70 μl of CIC Antigen Buffer Dissociation Neutralizer.
[0096] Wherein, every 1L of CIC separation agent includes the following components: 6.4g of borax, 5.1g of boric acid, 80g of polyethylene glycol, 7.7g of sodium chloride, 200μl of liquid biological preservative, and the balance is distilled water.
[0097] Each 1L of CIC detergent includes the following components: 6.4g of borax, 5.1g of boric acid, 40g of polyethylene glycol, 9.9g of sodium chloride, 200μl of liquid biological preservative, and the balance is distilled water.
[0098] Each 1 L of CIC reconstitution solvent includes the following components: 9.0 g of sodium chloride, 0.016 g of sodium h...
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