A kind of immune complex buffer dissociation agent and its application

A technology of immune complex and dissociation agent, applied in the field of medical detection, can solve problems such as good repeatability

Inactive Publication Date: 2016-04-06
中国人民解放军联勤保障部队第九0三医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the technical problems that the existing CIC detection technology and detection process cannot simultaneously have specificity, sensitivity, batch efficiency, good repeatability, and no interference, the invention discloses an immune complex buffer dissociation agent, and also discloses the The field of application of a kind of immune complex buffer dissociation agent

Method used

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  • A kind of immune complex buffer dissociation agent and its application
  • A kind of immune complex buffer dissociation agent and its application
  • A kind of immune complex buffer dissociation agent and its application

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0059]An immune complex buffer dissociation agent, based on the following volume ratio, each component and content are: 135 μl of CIC separation agent, 290 μl of CIC detergent, 5 μl of CIC reconstitution solvent, 65 μl of CIC antigen buffer solution Dissociating agent and 65 μl of CIC antigen buffer to dissociate neutralizing agent.

[0060] Wherein, every 1 L of CIC separation agent includes the following components: 5.2 g of borax, 4.1 g of boric acid, 70 g of polyethylene glycol, 6.2 g of sodium chloride, 100 μl of liquid biological preservative, and distilled water as the balance.

[0061] Each 1 L of CIC detergent includes the following components: 5.2 g of borax, 4.1 g of boric acid, 35 g of polyethylene glycol, 8.0 g of sodium chloride, 100 μl of liquid biological preservative, and distilled water as the balance.

[0062] Each 1 L of CIC reconstitution solvent includes the following components: 8.0 g of sodium chloride, 0.01 g of sodium hydroxide, 50 μl of polyethylene ...

Embodiment 2

[0074] An immune complex buffer dissociation agent, based on the following volume ratio, each component and content are: 165 μl of CIC separation agent, 315 μl of CIC detergent, 16 μl of CIC reconstitution solvent, 76 μl of CIC antigen buffer solution Dissociating agent and 76 μl of CIC antigen buffer to dissociate neutralizing agent.

[0075] Wherein, every 1L of CIC separation agent includes the following components: 7.7g of borax, 6.1g of boric acid, 90g of polyethylene glycol, 9.2g of sodium chloride, 500μl of liquid biological preservative, and the balance is distilled water.

[0076] Each 1L of CIC detergent includes the following components: 7.7g of borax, 6.1g of boric acid, 45g of polyethylene glycol, 11.7g of sodium chloride, 500μl of liquid biological preservative, and the balance is distilled water.

[0077] Each 1 L of CIC reconstitution solvent includes the following components: 9.5 g of sodium chloride, 0.03 g of sodium hydroxide, 500 μl of polyethylene glycol o...

Embodiment 3

[0089] An immune complex buffer dissociation agent, based on the following volume ratio, each component and content are: 150 μl of CIC separation agent, 300 μl of CIC detergent, 10 μl of CIC reconstitution solvent, 70 μl of CIC antigen buffer solution Dissociating agent and 70 μl of CIC antigen buffer to dissociate neutralizing agent.

[0090] Wherein, every 1L of CIC separation agent includes the following components: 6.4g of borax, 5.1g of boric acid, 80g of polyethylene glycol, 7.7g of sodium chloride, 200μl of liquid biological preservative, and the balance is distilled water.

[0091] Each 1L of CIC detergent includes the following components: 6.4g of borax, 5.1g of boric acid, 40g of polyethylene glycol, 9.9g of sodium chloride, 200μl of liquid biological preservative, and the balance is distilled water.

[0092] Each 1 L of CIC reconstitution solvent includes the following components: 9.0 g of sodium chloride, 0.016 g of sodium hydroxide, 300 μl of polyethylene glycol o...

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Abstract

The invention relates to the field of medical detection, and discloses an immune complex buffer dissociation agent which comprises a CIC (circulating immune complex) separating agent, a CIC cleaning agent, a CIC redissolving agent, a CIC antigen (or antibody) buffer solution dissociation agent and a CIC antigen (or antibody) buffer solution dissociation neutralizer. The invention also discloses a method for detecting CIC by using the immune complex buffer dissociation agent, which comprises the following steps: separating, washing, redissolving, dissociating the CIC, neutralizing, and finally detecting the antigen or antibody in the sample CIC. The method for detecting CIC by using the immune complex buffer dissociation agent establishes a standard operation process for specifically detecting the antigen in the CIC and a standard operation process for specifically detecting the antibody in the CIC, which is the problem that can not be solved by the existing CIC detection methods.

Description

technical field [0001] The invention relates to the field of medical detection, in particular to an immune complex buffer dissociation agent, and in particular to the application of the immune complex buffer dissociation agent to detect CIC. Background technique [0002] After bacteria, viruses and other pathogens invade the body or allergens (antigens, including self-antigens) stimulate the body to generate an immune response, and then produce specific immune effector cells or antibodies, and specifically bind to the antigen, the resulting complex is called Immune complexes (also known as antigen-antibody complexes, immunecomplex, IC) mainly include IgG, IgM, IgA, and IgE type immune complexes, among which IgG and IgM are the most common. Due to the different ratios of antigen and antibody, the formed IC molecules have different sizes, usually in three forms: First, when the ratio of antigen to antibody is appropriate, large insoluble ICs (greater than 19S) are formed, whic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/564G01N33/531
CPCG01N33/54393G01N33/564G01N2800/50G01N2800/52
Inventor 成军戴玉柱闫利斯友良王国政孙长贵
Owner 中国人民解放军联勤保障部队第九0三医院
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