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CIC (circulating immune complex) antibody buffer solution dissociation neutralizer for immune complex buffer dissociation agent

An immune complex and buffer technology, applied in the field of medical detection, can solve the problems of good repeatability and high batch efficiency, and achieve the effect of good repeatability and improved work efficiency.

Inactive Publication Date: 2014-05-07
THE PLA 117 HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the technical problems that the existing CIC detection technology and detection process cannot simultaneously have specificity, sensitivity, batch efficiency, good repeatability, and no interference, the present invention discloses a buffer for immune complexes that solves the above technical problems CIC Antibody Buffer for Dissociating Agents Dissociating Neutralizers

Method used

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  • CIC (circulating immune complex) antibody buffer solution dissociation neutralizer for immune complex buffer dissociation agent
  • CIC (circulating immune complex) antibody buffer solution dissociation neutralizer for immune complex buffer dissociation agent
  • CIC (circulating immune complex) antibody buffer solution dissociation neutralizer for immune complex buffer dissociation agent

Examples

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Effect test

Embodiment 1

[0042] An immune complex buffer dissociation agent, based on the following volume ratio, each component and content are: 135 μl of CIC separation agent, 290 μl of CIC detergent, 5 μl of CIC reconstitution solvent, 65 μl of CIC antibody buffer solution Dissociating agent and 65 μl of CIC antibody buffer to dissociate neutralizing agent.

[0043] Wherein, every 1 L of CIC separation agent includes the following components: 5.2 g of borax, 4.1 g of boric acid, 70 g of polyethylene glycol, 6.2 g of sodium chloride, 100 μl of liquid biological preservative, and distilled water as the balance.

[0044] Each 1 L of CIC detergent includes the following components: 5.2 g of borax, 4.1 g of boric acid, 35 g of polyethylene glycol, 8.0 g of sodium chloride, 100 μl of liquid biological preservative, and distilled water as the balance.

[0045] Each 1 L of CIC reconstitution solvent includes the following components: 8.0 g of sodium chloride, 0.01 g of sodium hydroxide, 50 μl of polyethyle...

Embodiment 2

[0057] An immune complex buffer dissociation agent, based on the following volume ratio, each component and content are: 165 μl of CIC separation agent, 315 μl of CIC detergent, 16 μl of CIC reconstitution solvent, 76 μl of CIC antibody buffer solution Dissociating agent and 76 μl of CIC antibody buffer to dissociate neutralizing agent.

[0058] Wherein, every 1L of CIC separation agent includes the following components: 7.7g of borax, 6.1g of boric acid, 90g of polyethylene glycol, 9.2g of sodium chloride, 500μl of liquid biological preservative, and the balance is distilled water.

[0059] Each 1L of CIC detergent includes the following components: 7.7g of borax, 6.1g of boric acid, 45g of polyethylene glycol, 11.7g of sodium chloride, 500μl of liquid biological preservative, and the balance is distilled water.

[0060] Each 1 L of CIC reconstitution solvent includes the following components: 9.5 g of sodium chloride, 0.03 g of sodium hydroxide, 500 μl of polyethylene glycol...

Embodiment 3

[0072] An immune complex buffer dissociation agent, based on the following volume ratio, each component and content are: 150 μl of CIC separation agent, 300 μl of CIC detergent, 10 μl of CIC reconstitution solvent, 70 μl of CIC antibody buffer solution Dissociating agent and 70 μl of CIC antibody buffer to dissociate neutralizing agent.

[0073] Wherein, every 1L of CIC separation agent includes the following components: 6.4g of borax, 5.1g of boric acid, 80g of polyethylene glycol, 7.7g of sodium chloride, 200μl of liquid biological preservative, and the balance is distilled water.

[0074] Each 1L of CIC detergent includes the following components: 6.4g of borax, 5.1g of boric acid, 40g of polyethylene glycol, 9.9g of sodium chloride, 200μl of liquid biological preservative, and the balance is distilled water.

[0075] Each 1 L of CIC reconstitution solvent includes the following components: 9.0 g of sodium chloride, 0.016 g of sodium hydroxide, 300 μl of polyethylene glycol...

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Abstract

The invention relates to the field of medical detection, and discloses a CIC (circulating immune complex) antibody buffer solution dissociation neutralizer for an immune complex buffer dissociation agent. Every 1L of the CIC antibody buffer solution dissociation neutralizer comprises 6.8-10.0g of trometamol, 6.5-9.6g of sodium chloride, 100-500 mu l of liquid biological preservative and the balance of distilled water. The method for detecting CIC by using the immune complex buffer dissociation agent establishes a standard operation process for specifically detecting the antibody in the CIC, which is the problem that can not be solved by the existing CIC detection methods at present. The immune complex buffer dissociation agent has the technical characteristics of high specificity and sensitivity, high efficiency and favorable repetitiveness, is free from interference, and can perform quantitative or qualitative detection. The immune complex buffer dissociation agent is used for detecting CIC in body fluid samples of patients with infectious diseases, incretion metabolic diseases, autoimmune diseases and part of neoplastic diseases.

Description

technical field [0001] The invention relates to the field of medical detection, in particular to a CIC antibody buffer dissociation neutralizer used for immune complex buffer dissociation agents. Background technique [0002] After bacteria, viruses and other pathogens invade the body or allergens (antigens, including self-antigens) stimulate the body to generate an immune response, and then produce specific immune effector cells or antibodies, and specifically bind to the antigen, the resulting complex is called Immune complexes (also known as antigen-antibody complexes, immune complexes, IC) mainly include IgG, IgM, IgA, and IgE type immune complexes, among which IgG and IgM are the most common. Due to the different ratios of antigen and antibody, the formed IC molecules have different sizes, usually in three forms: First, when the ratio of antigen to antibody is appropriate, large insoluble ICs (greater than 19S) are formed, which are easily captured, phagocytized and cle...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/564G01N33/531
CPCG01N33/564G01N33/54393G01N2800/50G01N2800/52
Inventor 成军江晓肖张益明马炬明陈达伟孙长贵戴玉柱王国政
Owner THE PLA 117 HOSPITAL
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