CIC (circulating immune complex) redissolving agent for immune complex buffer dissociation agent

A technology of immune complexes and reconstitution agents, which is applied in the field of medical testing and can solve the problems of specificity, sensitivity and repeatability

Inactive Publication Date: 2014-05-07
中国人民解放军联勤保障部队第九0三医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the technical problems that the existing CIC detection technology and detection process cannot simultaneously have specificity, sensitivity, batch efficiency, good repeatability, and no interference, the present invention discloses a buffer for immune complexes that solves the above technical problems CIC Reconstitution Solvent for Dissociating Agents

Method used

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  • CIC (circulating immune complex) redissolving agent for immune complex buffer dissociation agent
  • CIC (circulating immune complex) redissolving agent for immune complex buffer dissociation agent
  • CIC (circulating immune complex) redissolving agent for immune complex buffer dissociation agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] An immune complex buffer dissociation agent, for every 150 μl of pleural and ascites samples to be tested, add the immune complex buffer dissociation agent with the following components: 135 μl of CIC separation agent, 290 μl of CIC detergent, 5 μl of CIC reconstitution solvent, 65 μl CIC Antigen Buffer Dissociation Reagent and 65 μl CIC Antigen Buffer Dissociation Neutralizer.

[0066] Wherein, every 1 L of CIC separation agent includes the following components: 5.2 g of borax, 4.1 g of boric acid, 70 g of polyethylene glycol, 6.2 g of sodium chloride, 100 μl of liquid biological preservative, and distilled water as the balance.

[0067] Each 1 L of CIC detergent includes the following components: 5.2 g of borax, 4.1 g of boric acid, 35 g of polyethylene glycol, 8.0 g of sodium chloride, 100 μl of liquid biological preservative, and distilled water as the balance.

[0068] Each 1 L of CIC reconstitution solvent includes the following components: 8.0 g of sodium chlorid...

Embodiment 2

[0080] An immune complex buffer dissociation agent, for every 150 μl of cerebrospinal fluid sample to be tested, add the immune complex buffer dissociation agent with the following components, 165 μl of CIC separation agent, 315 μl of CIC detergent, 16 μl of CIC reconstitution solvent, 76 μl of CIC Antigen Buffer Dissociation Agent and 76 μl of CIC Antigen Buffer Dissociation Neutralizer.

[0081] Wherein, every 1L of CIC separation agent includes the following components: 7.7g of borax, 6.1g of boric acid, 90g of polyethylene glycol, 9.2g of sodium chloride, 500μl of liquid biological preservative, and the balance is distilled water.

[0082] Each 1L of CIC detergent includes the following components: 7.7g of borax, 6.1g of boric acid, 45g of polyethylene glycol, 11.7g of sodium chloride, 500μl of liquid biological preservative, and the balance is distilled water.

[0083] Each 1 L of CIC reconstitution solvent includes the following components: 9.5 g of sodium chloride, 0.03...

Embodiment 3

[0095]An immune complex buffer dissociation agent, add the following components of immune complex buffer dissociation agent for every 150 μl of the sample to be tested, 150 μl of CIC separation agent, 300 μl of CIC detergent, 10 μl of CIC reconstitution solvent, 70 μl of CIC Antigen Buffer Dissociation Agent and 70 μl of CIC Antigen Buffer Dissociation Neutralizer.

[0096] Wherein, every 1 L of CIC separation agent includes the following components: 6.4 g of borax, 5.1 g of boric acid, 80 g of polyethylene glycol, 7.7 g of sodium chloride, 200 μl of liquid biological preservative, and distilled water as the balance.

[0097] Each 1L of CIC detergent includes the following components: 6.4g of borax, 5.1g of boric acid, 40g of polyethylene glycol, 9.9g of sodium chloride, 200μl of liquid biological preservative, and the balance is distilled water.

[0098] Each 1 L of CIC reconstitution solvent includes the following components: 9.0 g of sodium chloride, 0.016 g of sodium hydro...

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Abstract

The invention relates to the field of medical detection, and discloses a CIC (circulating immune complex) redissolving agent for an immune complex buffer dissociation agent. Every 1L of the CIC redissolving agent comprises 8.0-9.5g of sodium chloride, 0.01-0.03g of sodium hydroxide, 50-500 mu l of polyethylene glycol octyl phenyl ether, 100-500 mu l of liquid biological preservative and the balance of distilled water. The method for detecting CIC by using the immune complex buffer dissociation agent establishes a standard operation process for specifically detecting the antigen in the CIC and a standard operation process for specifically detecting the antibody in the CIC, which is the problem that can not be solved by the existing CIC detection methods. The immune complex buffer dissociation agent is used for detecting CIC in body fluid samples of patients with infectious diseases, incretion metabolic diseases, autoimmune diseases and part of neoplastic diseases.

Description

technical field [0001] The invention relates to the field of medical detection, in particular to a CIC reconstitution agent used for buffering dissociation agents of immune complexes. Background technique [0002] After bacteria, viruses and other pathogens invade the body or allergens (antigens, including self-antigens) stimulate the body to generate an immune response, and then produce specific immune effector cells or antibodies, and specifically bind to the antigen, the resulting complex is called Immune complexes (also known as antigen-antibody complexes, immune complexes, IC) mainly include IgG, IgM, IgA, and IgE type immune complexes, among which IgG and IgM are the most common. Due to the different ratios of antigen and antibody, the formed IC molecules have different sizes, usually in three forms: First, when the ratio of antigen to antibody is appropriate, large insoluble ICs (greater than 19S) are formed, which are easily captured, phagocytized and cleared by phag...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/564
CPCG01N33/54393G01N33/564G01N2800/50G01N2800/52
Inventor 成军孙长贵戴玉柱王国政陈达伟江晓肖马炬明张益明
Owner 中国人民解放军联勤保障部队第九0三医院
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