Extraction method for chicken intestine antibacterial peptide

An extraction method and technology of antimicrobial peptides, applied in the direction of peptides, etc., can solve the problems of complex antimicrobial peptide methods, difficulties in separation and purification, and achieve the effect of simple and easy-to-understand extraction methods

Inactive Publication Date: 2014-06-25
QINGDAO ZHONGREN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0007] In order to overcome the above-mentioned problems existing in the prior art field, the object of the present invention is to provide a method for extracting chicken intestinal antimicrobial peptides, which solves the problems that the current methods for extracting antimicrobial peptides are relatively complicated and difficult to separate and purify

Method used

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Embodiment

[0016] The extraction method of the chicken intestinal antimicrobial peptide of the present embodiment, it comprises following specific steps:

[0017] (1) Intestinal mucosa and bacterial strains: The intestinal mucosa and E. coli strains of 60-day-old three-yellow chickens raised in a certain chicken farm were collected;

[0018] (2) Preparation of crude antimicrobial peptides: take fresh chicken intestinal mucosa and wash it with sterilized saline, mash it with a tissue grinder, add 5% acetic acid at a ratio of 1:1, bathe in water at 100°C for 10 minutes, and cool rapidly at 4°C , Centrifuge at 8000r / min for 30min, take the supernatant and ultrasonically crack it for 30s, stir and extract at 4°C for 24h, repeat the above centrifugation process, take the supernatant, adjust the pH value to 7.0, centrifuge again, and freeze-dry the supernatant;

[0019] (3) Purification of crude antimicrobial peptides: put the supernatant on SephadexG-50 filter chromatography column, elute wit...

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Abstract

A disclosed extraction method for chicken intestine antibacterial peptide comprises the following specific steps: (1) taking of intestinal mucosa and bacterial strains; (2) preparation of an antibacterial peptide crude product; and (3) purification of the antibacterial peptide crude product. The extraction method is simple and easily understandable. The extracted antibacterial peptide widely exists in living bodies, has activity of resisting bacteria, resisting virus, killing parasites, resisting tumors and the like, is an extremely important constituent composition of a congenital immune system, and is capable of resisting invasion of foreign microbes.

Description

[0001] technical field [0002] The invention belongs to an extraction method of antibacterial peptides, in particular to an extraction method of chicken intestinal antibacterial peptides. [0003] Background technique [0004] Antimicrobial peptide refers to a class of basic polypeptide substances with a molecular weight of about 4KD and antibacterial activity produced by induction in insects. So far, many proteins and peptides with antibacterial effects have been found in different animal tissues, and the structures of more than 70 antibacterial peptides have been determined, and the concept of antibacterial peptides has been greatly expanded. [0005] Antimicrobial peptides are small molecular polypeptides with broad-spectrum antibacterial activity induced in organisms. These polypeptides are non-specific immune response products of organisms and are important components of the immune system in organisms. Recent studies have found that antimicrobial peptides have specia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K2/00C07K1/36C07K1/34C07K1/16
Inventor 张述智夏伟朱绍辉张浩王晓丽徐权汗李之详许团辉
Owner QINGDAO ZHONGREN PHARMA
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