A phosphorus accumulating bacterium with high phosphorus removal effect at low temperature and its screening method and application

A technology of phosphorus-accumulating bacteria and low temperature, applied in phosphorus-accumulating bacteria and its screening method and application field, can solve the problems of no cultivation and domestication methods, no identification methods, etc., and achieve the effect of wide temperature adaptability and high phosphorus removal activity

Active Publication Date: 2016-02-10
ANHUI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research on such bacteria and their phosphorus removal characteristics is still in its infancy, and there is no quick way to cultivate and domesticate it, and there is no systematic identification method

Method used

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  • A phosphorus accumulating bacterium with high phosphorus removal effect at low temperature and its screening method and application
  • A phosphorus accumulating bacterium with high phosphorus removal effect at low temperature and its screening method and application
  • A phosphorus accumulating bacterium with high phosphorus removal effect at low temperature and its screening method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Example 1 Isolation, screening and identification of bacterial strains of the present invention:

[0035] (1) Culture medium

[0036] Medium A: Each liter of medium contains 10 g of peptone, 5 g of beef extract, 5 g of sodium chloride, and 19 g of agar; the balance is water; pH 7.0-7.2.

[0037] Medium A is used for strain isolation, purification and preservation.

[0038] Medium B: 2g sodium acetate (carbon source) per liter of medium, Na 2 HPO4 46mg, NH 4 Cl152.8mg, CaCl 2 8.3mg, MgSO 4 82mg, K 2 SO 4 17.83mg, HEPES7g, trace elements 2mL; the balance is water, pH7.0-7.2.

[0039] Medium B was used as a pre-medium for the strains.

[0040] Medium C: 1 g of sodium acetate (carbon source) per liter of medium, MgSO 4 82mg, FeSO 4 3.7mg, CaCl 2 60mg, (NH 4 ) 2 SO 4 0.2g, beef paste 0.22g, K 2 HPO 4 74mg, trace elements 2mL; the balance is water; pH7.0-7.2.

[0041] Medium C was used for phosphorus-enriched cultivation of the strain.

[0042] D. Compositio...

Embodiment 2

[0061] The optimum phosphorus removal condition of embodiment 2 bacterial strain of the present invention

[0062] 1. The seed liquid obtained by culturing the strains preserved in the present invention is inoculated into medium C at a volume ratio of 1:10, and shaken for 48 hours at 8°C, 10°C, 15°C, 20°C, 25°C, and 30°C, respectively. Among them, the anaerobic culture was firstly cultured for 24 hours, and then the aerobic culture was cultured for 24 hours. After 48 hours, the OD value and phosphorus removal rate of medium C were detected. The detection results are as follows: figure 1 shown. Depend on figure 1 The results show that the strain of the present invention has a wide range of temperature adaptability, has a good phosphorus accumulation effect at 8-30°C, and can be used for wastewater treatment in winter or under low temperature conditions.

[0063] 2. The seed liquid obtained by culturing the strains preserved in the present invention is inoculated into phosphor...

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Abstract

The invention discloses a phosphorus-accumulating bacterium strain with higher phosphorus removal effect at low temperature, which is characterized in that the collection number of the phosphorus-accumulating bacterium is CCTCC NO:M2014140. The phosphorus-accumulating bacterium strain is screened from Chaohu Lake sediment by low-temperature enrichment culture and a dilution mixed plate method, and can efficiently remove phosphorus at low temperature; the strain can still effectively absorb the carbon source in wastewater at 8 DEG C and convert the carbon source into poly-beta-polyhydroxyalkanoats, and meanwhile, release polyphosphates stored in the cells so as to obtain energy; the poly-beta-polyhydroxyalkanoats in the cells are oxidized in the aerobic process to generate energy, and the strain absorbs abundant phosphorus from the wastewater to as to accumulate the phosphorus in the form of polyphosphates. The strain has wide temperature adaptability, has favorable phosphorus-accumulating effect at 8-30 DEG C, and can be used for wastewater treatment in winter or under cold conditions.

Description

technical field [0001] The invention relates to a phosphorus accumulating bacterium with high phosphorus removal effect at low temperature, a screening method and application thereof, and belongs to the field of environmental microorganisms. Background technique [0002] Water pollution is an important problem faced by all countries in the world, and this problem is still serious in a developing country like my country. In recent decades, my country's population has grown rapidly, urbanization has accelerated, urban sewage discharge has increased, and water pollution is very serious and has a clear trend of deterioration. With the increasing water pollution, the problem of water eutrophication is becoming more and more prominent. In the past ten years, the proportion of eutrophic water bodies in my country has increased from 50% to 55%, while the proportion of eutrophic water bodies has dropped from 3.2% to 0.53%. The eutrophication of the water body will cause the mass re...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/38
Inventor王宁邵啸吴涓李玉成毕潇
OwnerANHUI UNIVERSITY