A phosphorus accumulating bacterium with high phosphorus removal effect at low temperature and its screening method and application
A technology of phosphorus-accumulating bacteria and low temperature, applied in phosphorus-accumulating bacteria and its screening method and application field, can solve the problems of no cultivation and domestication methods, no identification methods, etc., and achieve the effect of wide temperature adaptability and high phosphorus removal activity
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Embodiment 1
[0034] Example 1 Isolation, screening and identification of bacterial strains of the present invention:
[0035] (1) Culture medium
[0036] Medium A: Each liter of medium contains 10 g of peptone, 5 g of beef extract, 5 g of sodium chloride, and 19 g of agar; the balance is water; pH 7.0-7.2.
[0037] Medium A is used for strain isolation, purification and preservation.
[0038] Medium B: 2g sodium acetate (carbon source) per liter of medium, Na 2 HPO4 46mg, NH 4 Cl152.8mg, CaCl 2 8.3mg, MgSO 4 82mg, K 2 SO 4 17.83mg, HEPES7g, trace elements 2mL; the balance is water, pH7.0-7.2.
[0039] Medium B was used as a pre-medium for the strains.
[0040] Medium C: 1 g of sodium acetate (carbon source) per liter of medium, MgSO 4 82mg, FeSO 4 3.7mg, CaCl 2 60mg, (NH 4 ) 2 SO 4 0.2g, beef paste 0.22g, K 2 HPO 4 74mg, trace elements 2mL; the balance is water; pH7.0-7.2.
[0041] Medium C was used for phosphorus-enriched cultivation of the strain.
[0042] D. Compositio...
Embodiment 2
[0061] The optimum phosphorus removal condition of embodiment 2 bacterial strain of the present invention
[0062] 1. The seed liquid obtained by culturing the strains preserved in the present invention is inoculated into medium C at a volume ratio of 1:10, and shaken for 48 hours at 8°C, 10°C, 15°C, 20°C, 25°C, and 30°C, respectively. Among them, the anaerobic culture was firstly cultured for 24 hours, and then the aerobic culture was cultured for 24 hours. After 48 hours, the OD value and phosphorus removal rate of medium C were detected. The detection results are as follows: figure 1 shown. Depend on figure 1 The results show that the strain of the present invention has a wide range of temperature adaptability, has a good phosphorus accumulation effect at 8-30°C, and can be used for wastewater treatment in winter or under low temperature conditions.
[0063] 2. The seed liquid obtained by culturing the strains preserved in the present invention is inoculated into phosphor...
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