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Phosphorus-accumulating bacterium strain with higher phosphorus removal at low temperature, and screening method and application thereof

A phosphorus-accumulating bacteria and low-temperature technology, applied in phosphorus-accumulating bacteria and its screening method and application field, can solve the problems of no identification method, no cultivation and domestication method, etc., and achieve the effect of wide temperature adaptability and high phosphorus removal activity

Active Publication Date: 2014-07-30
ANHUI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the research on such bacteria and their phosphorus removal characteristics is still in its infancy, and there is no quick way to cultivate and domesticate it, and there is no systematic identification method

Method used

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  • Phosphorus-accumulating bacterium strain with higher phosphorus removal at low temperature, and screening method and application thereof
  • Phosphorus-accumulating bacterium strain with higher phosphorus removal at low temperature, and screening method and application thereof
  • Phosphorus-accumulating bacterium strain with higher phosphorus removal at low temperature, and screening method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0034] The isolation screening and identification of embodiment 1 bacterial strain of the present invention:

[0035] (1) culture medium

[0036] Medium A: Each liter medium contains 10g of peptone, 5g of beef extract, 5g of sodium chloride, and 19g of agar; the balance is water; pH7.0-7.2.

[0037] Medium A was used for strain isolation, purification and preservation.

[0038] Medium B: 2g of sodium acetate (carbon source) per liter of medium, Na 2 HPO4 46 mg, NH 4 Cl152.8mg, CaCl 2 8.3 mg, MgSO 4 82 mg, K 2 SO 4 17.83mg, HEPES7g, trace elements 2mL; the balance is water, pH7.0-7.2.

[0039] Medium B was used as a pre-medium for the strains.

[0040] Medium C: 1g of sodium acetate (carbon source) per liter of medium, MgSO 4 82mg, FeSO 4 3.7 mg, CaCl 2 60mg, (NH 4 ) 2 SO 4 0.2g, beef extract 0.22g, K 2 HPO 4 74mg, trace elements 2mL; the balance is water; pH7.0-7.2.

[0041] Medium C was used for the phosphorus-enriched cultivation of the strains.

[0042] D....

Embodiment 2

[0061] The optimal phosphorus removal condition of embodiment 2 bacterial strains of the present invention

[0062] 1. Inoculate the seed liquid obtained by culturing the preserved bacterial strains of the present invention in medium C at a volume ratio of 1:10, and shake and culture at 8°C, 10°C, 15°C, 20°C, 25°C, and 30°C for 48 hours, Among them, first anaerobic culture for 24 hours, then aerobic culture for 24 hours, after 48 hours, the OD value and phosphorus removal rate of medium C were detected, and the detection results were as follows: figure 1 shown. Depend on figure 1 The results show that the bacterial strain of the present invention has wide temperature adaptability, and has good phosphorus accumulation effect at 8-30°C, and can be used for wastewater treatment in winter or under low temperature conditions.

[0063] 2. Inoculate the seed solution obtained by culturing the preserved bacterial strains of the present invention in phosphorus-rich medium with differ...

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Abstract

The invention discloses a phosphorus-accumulating bacterium strain with higher phosphorus removal effect at low temperature, which is characterized in that the collection number of the phosphorus-accumulating bacterium is CCTCC NO:M2014140. The phosphorus-accumulating bacterium strain is screened from Chaohu Lake sediment by low-temperature enrichment culture and a dilution mixed plate method, and can efficiently remove phosphorus at low temperature; the strain can still effectively absorb the carbon source in wastewater at 8 DEG C and convert the carbon source into poly-beta-polyhydroxyalkanoats, and meanwhile, release polyphosphates stored in the cells so as to obtain energy; the poly-beta-polyhydroxyalkanoats in the cells are oxidized in the aerobic process to generate energy, and the strain absorbs abundant phosphorus from the wastewater to as to accumulate the phosphorus in the form of polyphosphates. The strain has wide temperature adaptability, has favorable phosphorus-accumulating effect at 8-30 DEG C, and can be used for wastewater treatment in winter or under cold conditions.

Description

technical field [0001] The invention relates to a phosphorus-accumulating bacterium with high phosphorus removal effect at low temperature, a screening method and application thereof, belonging to the field of environmental microorganisms. Background technique [0002] The problem of water pollution is an important problem faced by all countries in the world, and this problem is still serious in a developing country like my country. In recent decades, my country's population has grown rapidly, the pace of urbanization has accelerated, and the amount of urban sewage discharge has increased day by day. The current situation of water pollution is very serious, and there is a clear trend of deterioration. With the aggravation of water pollution, the problem of eutrophication of water body is becoming more and more prominent. In the past ten years, the proportion of eutrophic water bodies in my country has increased from 50% to 55%, while the proportion of oligotrophic water bod...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/38
Inventor 王宁邵啸吴涓李玉成毕潇
Owner ANHUI UNIVERSITY
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