Phosphorus-accumulating bacterium strain with higher phosphorus removal at low temperature, and screening method and application thereof

A phosphorus-accumulating bacteria and low-temperature technology, applied in phosphorus-accumulating bacteria and its screening method and application field, can solve the problems of no identification method, no cultivation and domestication method, etc., and achieve the effect of wide temperature adaptability and high phosphorus removal activity

Active Publication Date: 2014-07-30
ANHUI UNIVERSITY
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

However, the research on such bacteria and their phosphorus removal characteristics is still in its infancy,

Method used

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  • Phosphorus-accumulating bacterium strain with higher phosphorus removal at low temperature, and screening method and application thereof
  • Phosphorus-accumulating bacterium strain with higher phosphorus removal at low temperature, and screening method and application thereof
  • Phosphorus-accumulating bacterium strain with higher phosphorus removal at low temperature, and screening method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0034] Example 1 Isolation, screening and identification of the strain of the present invention:

[0035] (1) Medium

[0036] Medium A: Each liter of medium contains 10g peptone, 5g beef extract, 5g sodium chloride, 19g agar; the balance is water; pH 7.0-7.2.

[0037] Medium A is used for strain isolation, purification, and preservation.

[0038] Medium B: Each liter of medium contains sodium acetate (carbon source) 2g, Na 2 HPO 4 46mg, NH 4 Cl152.8mg, CaCl 2 8.3mg, MgSO 4 82mg, K 2 SO 4 17.83mg, HEPES 7g, trace elements 2mL; the balance is water, pH 7.0-7.2.

[0039] Medium B is used as a pre-medium for the strain.

[0040] Medium C: Each liter of medium contains sodium acetate (carbon source) 1g, MgSO 4 82mg, FeSO 4 3.7mg, CaCl 2 60mg, (NH 4 ) 2 SO 4 0.2g, beef extract 0.22g, K 2 HPO 4 74mg, 2mL of trace elements; the balance is water; pH 7.0-7.2.

[0041] Medium C is used for the phosphorus-rich culture of the strain.

[0042] D. Trace element composition (g / L): FeCl per liter of medium...

Example Embodiment

[0061] Example 2 Optimal Phosphorus Removal Conditions of the Strain of the Invention

[0062] 1. The seed solution obtained by culturing the strains preserved in the present invention is inoculated into medium C at a volume ratio of 1:10, and cultured with shaking at 8°C, 10°C, 15°C, 20°C, 25°C, and 30°C for 48 hours. Among them, first anaerobic culture for 24 hours, and then aerobic culture for 24 hours, after 48 hours, the OD value and phosphorus removal rate of medium C were tested. figure 1 Shown. by figure 1 The results show that the strain of the present invention has wide temperature adaptability, has a good phosphorus accumulation effect at 8-30°C, and can be used for wastewater treatment in winter or under low temperature conditions.

[0063] 2. The seed solution obtained by culturing with the strains preserved in the present invention was inoculated into phosphorus-rich medium with different carbon sources at a volume ratio of 1:10, and cultured with shaking at 20°C for ...

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Abstract

The invention discloses a phosphorus-accumulating bacterium strain with higher phosphorus removal effect at low temperature, which is characterized in that the collection number of the phosphorus-accumulating bacterium is CCTCC NO:M2014140. The phosphorus-accumulating bacterium strain is screened from Chaohu Lake sediment by low-temperature enrichment culture and a dilution mixed plate method, and can efficiently remove phosphorus at low temperature; the strain can still effectively absorb the carbon source in wastewater at 8 DEG C and convert the carbon source into poly-beta-polyhydroxyalkanoats, and meanwhile, release polyphosphates stored in the cells so as to obtain energy; the poly-beta-polyhydroxyalkanoats in the cells are oxidized in the aerobic process to generate energy, and the strain absorbs abundant phosphorus from the wastewater to as to accumulate the phosphorus in the form of polyphosphates. The strain has wide temperature adaptability, has favorable phosphorus-accumulating effect at 8-30 DEG C, and can be used for wastewater treatment in winter or under cold conditions.

Description

technical field [0001] The invention relates to a phosphorus-accumulating bacterium with high phosphorus removal effect at low temperature, a screening method and application thereof, belonging to the field of environmental microorganisms. Background technique [0002] The problem of water pollution is an important problem faced by all countries in the world, and this problem is still serious in a developing country like my country. In recent decades, my country's population has grown rapidly, the pace of urbanization has accelerated, and the amount of urban sewage discharge has increased day by day. The current situation of water pollution is very serious, and there is a clear trend of deterioration. With the aggravation of water pollution, the problem of eutrophication of water body is becoming more and more prominent. In the past ten years, the proportion of eutrophic water bodies in my country has increased from 50% to 55%, while the proportion of oligotrophic water bod...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/38
Inventor 王宁邵啸吴涓李玉成毕潇
Owner ANHUI UNIVERSITY
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