Conical surface percolation type device and method for collecting root exudates of soil cultivation plants

Abstract

The invention discloses conical surface percolation type device and method for collecting root exudates of soil cultivation plants. The collection device comprises a growing box and a collection box, wherein holes are formed in the outer wall of the growing box and are used for filling agar. A collection hole is formed in a connection part of the cylindrical growing box and the lower end of the collection box; the lower end of the whole box body is provided with a rubber plug with a hole and is connected with a resin column by a guide pipe. The novel collection method for the root exudates of the soil cultivation plants comprises the following steps: firstly, enabling the plants to grow in soil of the growing box; enabling plant roots to penetrate through the agar to enter the collection box filled with quartz sand; treating and collecting the root exudates of the plants in the collection box. The conical surface percolation type device and method are suitable for researching the root exudates of the plants under soil cultivation conditions; the growth of the roots is not disturbed and a root environment is not damaged; a collection environment is close to a natural growing state of the plants; the collection method has a small occupied area and the available growing space of the plants is large. The method is low in cost and strong in practicability and has a very good application value.

Description

technical field [0001] The invention relates to the technical field of phytoremediation of soil polluted environments, in particular to a device and method for collecting soil-cultured plant root exudates by conical surface infiltration. Background technique [0002] At present, plant root exudates generally adopt the solution collection method. The specific operation method is: the plant root first grows in the nutrient solution, and then transfers to the treatment solution. After a period of treatment, the treatment solution is collected as root exudates. The solution collection method is simple to operate and convenient to analyze. However, the root exudates obtained by the solution collection method are difficult to reflect the plant root exudates under soil conditions. Because the soil composition is complex, the nutrient exists in various forms, and the distribution is uneven. Moreover, most of the effective nutrients are absorbed by the soil colloid and cannot diffus...

AI Technical Summary

Problems solved by technology

Among the plant root exudate collection devices and collection methods and collection devices under the soil cultivation conditions that are currently patented, some are cumbersome to use, some occupy a large area and use a small space, and the number of plants to be planted is very limited.

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