A kind of preservation method of duckweed living material

A preservation method and duckweed technology, applied in the fields of botanical equipment and methods, soilless culture, cultivation, etc., can solve the problems of high cost of material preservation, easy infection of green algae, inability to directly observe the natural form of duckweed, etc. cost of scientific research, avoidance of algal infection, and the effect of improving scientific research output

Active Publication Date: 2016-08-31
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is relatively simple, but it needs to be cultivated for at least two weeks when the duckweed plant material in normal form is used. This cultivation method is often limited by the natural growth season, and it is still easy to infect green algae in subsequent water cultivation.
[0007] The artificial climate box-solid medium aseptic preservation method can meet the requirements of fine experiments, but this method needs to be transferred to culture regularly, which requires high technical requirements for managers, and the management procedures are cumbersome, and it is dependent on artificial climate boxes and other instruments. Strong, high material storage cost, and difficult to expand storage capacity
Especially in recent years, it has been found that duckweed preserved in solid medium has completely changed its morphological characteristics due to its adaptation to this nutrient-rich, water-less environment. Physiological ecological and morphological indicators in the state (liquid culture medium)
For research projects that urgently need to observe the biological characteristics of natural growth of duckweed, it is necessary to remove duckweed from the sterile medium and cultivate it in liquid, but during the cultivation process, it is easy to infect green algae

Method used

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  • A kind of preservation method of duckweed living material
  • A kind of preservation method of duckweed living material
  • A kind of preservation method of duckweed living material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Such as Figure 1 ~ Figure 2 Shown, preserved duckweed material with the inventive method.

[0024] 1. Reagents and materials

[0025] Culture container: a clean, open, uncovered round bucket with a height of about 40 cm and a diameter of 30 cm.

[0026] Hoagland standard nutrient solution: KNO 3 1000mg / L,NH 4 NO 3 140mg / L, Ca(NO 3 )2.4H 2 O 600mg / L, MgSO 4 .7H 2 O 500mg / L, ferritartrate 5mg / L, Mncl 2 .4H 2 0mg / L, ZnSO 4 .7H 2 O 0.2mg / L, H 3 BO 3 3mg / L,MoO 3 0.07mg / L, CuSO 4 .5H 2 O 0.08mg / L. The pH was adjusted to 6.5.

[0027] Duckweed material: live plants of Lemna minor and Spirodela polyrhiza that are healthy and free from algae pollution.

[0028] Azolla imbricata material: choose healthy live plants of Azolla imbricata without algae pollution.

[0029] Light-transmitting net: 40 mesh white nylon net.

[0030] 2. Save operation method

[0031] Add 1 / 3 of the height of the container to the culture container, add 200ml of Hoagland standard ...

Embodiment 2

[0043] Preserve the duckweed material with the method of the present invention.

[0044] It is the same as Example 1 and will not be repeated, and the difference lies in the concentration of the nutrient solution and the ratio of the number of duckweed and Manjianghong.

[0045] 1. Reagents and materials are the same as in Example 1.

[0046] 2. Save operation method

[0047] Add 3.8L tap water to the culture container, add 200ml Hoagland standard nutrient solution, and mix well, which is equivalent to 20 times diluted Hogland standard nutrient solution;

[0048] Put 16 strains of duckweed (green duckweed and multiple purple duckweeds at random) and 4 strains of Manjianghong in the culture container, the ratio of the number of strains is 4:1.

[0049] All the other operations and management are the same as in Embodiment 1.

[0050] 3. The preservation effect is the same as that in Embodiment 1.

[0051] 4, duckweed material is taken with embodiment one.

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PUM

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Abstract

The invention discloses a living duckweed material storage method. The living duckweed material storage method aims to overcome the defects that according to a duckweed germplasm resource storage method in the prior art, duckweed germplasm resources are prone to being infected with alga, the method is limited by natural growth seasons and is high in cost, and clean research materials cannot be provided for precise experiments at any time. Duckweed and azolla are cultivated in a cultivation container at the same time, and the cultivation container is placed in the natural environment. Preferably, the ratio of the number of the duckweed to the number of the azolla is controlled to be 2:1-4:1, a Hogland standard nutrient solution diluted by 20-30 times is adopted as an initial culture solution, and tap water is added properly in the cultivation process. The method can permanently and effectively prevent pollution caused when the living duckweed is infected with the alga in the storage process, and clean research materials are provided for precise experiments. By means of the method, the duckweed can keep in the natural state at any time so that the demand for materials of scientific experiment research can be met at any time.

Description

technical field [0001] The invention relates to a method for preserving plant materials, in particular to a method for preserving living duckweed materials. The invention belongs to the technical fields of plant germplasm resource preservation, aquatic seed plant preservation and cultivation. Background technique [0002] Duckweed, generally refers to many different genera and species in Lemnaceae in the traditional plant classification system and Araceae in the current APGIII classification system, such as Lemnaminor and Spirodela polyrhiza Wait. Duckweed is the smallest flowering plant and has high basic research value. Duckweed is an important biological resource. As a new energy resource and an efficient plant for comprehensive management of the ecological environment, it has attracted widespread attention from scientists. The U.S. Department of Energy (USDOE) has listed duckweed as one of the most promising new energy plants. Therefore, duckweed not only has basic r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G31/00
Inventor 彭玉兰赵海高信芬
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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