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An early-stage detection kit for diabetic nephropathy, a biomarker detecting method and applications

A technology for early detection of diabetic nephropathy, applied in the field of early detection test paper boxes for diabetic nephropathy, can solve the problems of high technical difficulty, complications, and great trauma.

Inactive Publication Date: 2015-07-08
BIO TECH ACADEMY CHINA
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although renal biopsy can assist in the early diagnosis of diabetic nephropathy, the technique is invasive and will cause complications after operation, and it is technically difficult, so it cannot be used as a routine item in the examination of patients with diabetic nephropathy

Method used

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  • An early-stage detection kit for diabetic nephropathy, a biomarker detecting method and applications
  • An early-stage detection kit for diabetic nephropathy, a biomarker detecting method and applications
  • An early-stage detection kit for diabetic nephropathy, a biomarker detecting method and applications

Examples

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Embodiment 1

[0109] Example 1: Screening and verification of biomarkers for early diagnosis of diabetic nephropathy

[0110] 1. Experimental method

[0111] 1. Pathological screening

[0112] A total of 205 subjects with known physical conditions were selected, including 50 healthy controls and 155 patients with type 2 diabetes (all patients had diabetes for more than 2 years); 155 diabetic patients were divided according to proteinuria , including 61 cases of normal proteinuria, 52 cases of microalbuminuria, 42 cases of massive proteinuria. The MDRD GRF formula estimates glomerular filtration rate (eGFR).

[0113] Note: This study has been approved by the ethics review agency, and all subjects signed informed consent.

[0114] 2. Collection and processing of urine samples

[0115]The first urine sample (30 mL) excreted by the subject in the morning was collected in a sterile container, centrifuged at 8000 g for 30 min at 4°C, and the supernatant was collected and stored at -80°C for p...

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Abstract

An early-stage detection kit for diabetic nephropathy is disclosed. The kit comprises detection agents. The detection agents comprise two or three of a first detection agent, a second detection agent, a third detection agent and a fourth detection agent. The solute of the first detection agent is a first podocalyxin antibody. The solute of the second detection agent is a first IV type collagen antibody. The solute of the third detection agent is a first liver-type fatty acid-binding protein antibody. The solute of the fourth detection agent is a first neutrophils gelatinase-associated lipocalin antibody. The kit adopts two or three of podocalyxin, IV type collagen, liver-type fatty acid-binding protein and neutrophils gelatinase-associated lipocalin as biomarkers, and can provide assisted determination for renal injury according to actual detection results, thus providing assistance for differentiating early-stage diabetic nephropathy.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a test kit for early detection of diabetic nephropathy, a biomarker detection method and its application. Background technique [0002] Diabetic nephropathy is one of the most serious chronic microvascular complications of diabetes, which eventually leads to end-stage renal failure and is the main cause of death in diabetic patients. According to the statistics of the World Health Organization in 2013, about 347 million people in the world suffer from diabetes, and more than 80% of the deaths from diabetes occur in developing countries. At the same time, the World Health Organization predicts that by 2030, the number of deaths from diabetes worldwide will double, of which 30% to 40% of type 2 diabetes patients will develop diabetic nephropathy, and 20% to 40% of type 1 diabetes patients will develop diabetic nephropathy after 15 years. ~30 years later, diabetic nephropathy wi...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/577
CPCG01N33/68G01N33/577G01N2800/042G01N2800/347
Inventor 张贯京张俊然谷东风克里斯基捏·普拉纽克艾琳娜·古列莎波达别特·伊万刘遥峰卢卡·穆桑特杨轶石江波朱孔林
Owner BIO TECH ACADEMY CHINA
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