Xianfeng grass and polyacetylene compounds for prevention and treatment of coccidiosis
A technology for compounds, compositions, applied in applications, plant raw materials, animal repellents, etc., which can solve problems such as public health concerns
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example 1
[0142] Materials and methods
[0143] Chemical material
[0144] Maduramycin, PBS, hematoxylin and eosin-Y were purchased from Sigma-Aldrich (St Louis, MO, USA). Xianfengcao is ground into powder after drying in the shade. Mix this powder with specific chicken rations. The quality control of each batch of Xianfengcao powder is evaluated by HPLC.
[0145] Birds, diets and experimental design
[0146] The chicks of one-day-old Lohmann broiler chickens incubated in Taichung chicken farm (Taiwan) were marked with wings, weighed and randomly placed in the Petersime initial incubator unit immediately after delivery. On day 1 and 2 after hatching, the chickens were given free access to water and food. The diet was formulated by mixing a basal diet (chicken diet, Taiwan Sugar Company) with vehicle alone (control diet) or a specific dose of salty grass powder (BPP diet). In the polyalkyne experiment, the animals were administered 45 μg of the isolated polyalkyne per day. After g...
example 2
[0171] Figure 4 To show the effect of polyacetylene glycosides on the appearance of diseased birds and the clinical symptoms of chickens. Day-old chickens were fed a control diet for 14 days. On the 14th day, chickens were infected with vehicle (CTR) or Eimeria tenella (ET), and fed with control diet (CTR and ET group) and diet containing 45 μg / kg polyglycoside (ET). +CP) were fed for another 7 days. Observe the appearance of sick birds ( Figure 4 A), bloody stool ( Figure 4 B) and at low magnification ( Figure 4 C) and high magnification ( Figure 4 D) Observe the pathological condition of the cecum.
[0172] Figure 5 shows the effect of polyynyl glycosides on the pathology of chicken mucosa. Will Figure 4 The cecum of the same chicken was fixed with 10% formalin and embedded in paraffin ( Figure 5A ). Sections were stained with hematoxylin and eosin-Y ( Figures 5B-5C ). Cecal sections were imaged and mucosal severity was measured ( Figure 5B ) and degree...
example 3
[0174] In order to prevent, suppress and / or treat coccidiosis, a composition is administered to animals, which comprises an effective amount of Herba chinensis, an active ingredient thereof or an active compound isolated therefrom. Salmonella, an active ingredient thereof, or an active compound isolated therefrom can be added to animal feed. The active ingredient or active compound of Xianfengcao can be extracted from Xianfengcao plant.
[0175] Salmonella powder, an active ingredient thereof or an active compound isolated therefrom can be prepared in the form of capsules. The composition may be prepared as a suppository dosage form. A pharmaceutically acceptable carrier can be added to prepare a suitable dosage form.
[0176] Salmonella contains at least the following polyacetylene compounds A, A', B, B', C and C':
[0177]
[0178] as well as
[0179]
PUM
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