151 results about "Coccidioses" patented technology

The invention discloses an E.tenella protein disulfide linkage isomerase gene EtPDI (Clone ID is BW1-E06,and the Genbank accession number of is EF552214). The gene is connected with a procaryon expression vector pGEX-4T-2; a procaryon expression recombination plasmid pGEX-4T-EtPDI is constructed and is expressed in a colibacillus system; and most of the expressed recombining protein exists in a soluble form. The recombining protein 4T-EtPDI is purified to carry out SPS-PAGE and is transferred to a PVDF film; and antiserum of E.tenella oocyst oral immunized chicken is used as first resistance and goat anti-chicken IgG is used as second resistance to carry out Western-blot analysis, thereby indicating that the gene has certain antigen. The gene is used for preparing an anti-chicken coccidiosis drug and an anti-chicken coccidiosis vaccine.

The invention discloses a Chinese medicinal preparation for treating avian coccidiosis and its preparing process, wherein the medicament is prepared from the following constituents (by weight portions): sweet wormwood 200-400g, dichroa febrifuga lour 200-400g, Chinese pulsatilla root 100-300g, astragalus root 100-300g. The preparing process for the product comprises the steps of weighing, poaching, drying, disintegrating, sieving, extracting, filtering, concentrating, flocculating setting, encapsulating and sterilizing.

The invention relates to a kind of slow-releasing parenteral injection and the preparative way of it. The product can prevent coccidiosis of chickens and rabbits. The ingredients of the parenteral injection include: 3-6 shares (by weight) abrotani herba and kuhseng, 2-5 shares (by weight) wood anemone, agrimony and bupleurum root, 2-4 shares (by weight) beta-cyclodextrin. The preparative way of the product goes as follows: lixiviate the materials with 95% ethyl alocohol twice to get A1 solution; extract the residue with water twice to get A2 solution; remove the tannin in A1 and A2 by heating alternating with cooling; envelop the material with beta-cyclodextrin to get the product.

The invention discloses preparing and application of a probiotics-fermented traditional Chinese medicinal preparation for preventing and treating coccidiosis in a chicken. Traditional Chinese medicines comprise artemisia apiacea, antifebrile dichroa, herba verbenae, phellodendron, radix sophorae flavescentis, radix curcumae, cape jasmine, angelica sinensis, pericarpium citri reticulatae, honeysuckle and agrimonia pilosa. Effective traditional Chinese medicinal ingredients are extracted by three times of decoction; afterwards, a traditional Chinese medicinal culture medium substrate is prepared from the effective traditional Chinese medicinal ingredients; lactic acid bacteria PP (pediococcus pentosaceus) are inoculated in the traditional Chinese medicinal culture medium substrate; the inoculated traditional Chinese medicinal culture medium substrate is cultured to prepare the probiotics-fermented traditional Chinese medicinal preparation for preventing and treating the coccidiosis in the chicken. In comparison with the prior art, according to the preparing and the application of the probiotics-fermented traditional Chinese medicinal preparation for preventing and treating the coccidiosis in the chicken, a probiotics-fermented traditional Chinese medicinal aqueous extract is used as a target composition; not only are the further conversion and decomposition of a polysaccharide, starch, cellulose and pharmaceutical active ingredients in the traditional Chinese medicines promoted and are more effective active ingredients are generated, but also the activity of probiotics is improved; the growth and the release of the probiotics and metabolites thereof are facilitated; further, an anticoccidial treatment effect is obviously higher than that of a pure traditional Chinese medicinal preparation.

The invention relates to a traditional Chinese medicine composition for preventing and treating poultry coccidiosis and a preparation method of the traditional Chinese medicine composition. The medicine materials of the traditional Chinese medicine composition comprise antifebrile dichroa, radix sophorae flavescentis, flos chrysanthemi indici, garlic, cogongrass rhizome, carbonized sanguisorba root, hairyvein agrimony, golden cypress, dandelion, rhizoma atractylodis, fennel, astragalus mongholicus, coptis chinensis and radix bupleuri. By using the traditional Chinese medicine composition, the purpose of preventing and treating coccidiosis in chicken can be effectively achieved in the aspects of expelling parasite, preventing malaria, hemostasis, cooling blood, clearing heat, diminishing inflammation and nourishing. In addition, two technologies of extraction and superfine grinding are adopted in a manufacturing process so that the utilization rate and biological solubility of the traditional Chinese medicines are significantly improved. Water or mixed materials can be taken during administration so that the clinical application is more convenient. Furthermore, the traditional Chinese medicine composition is prepared from pure traditional Chinese medicines, has no drug residue and has no toxic and side effects on an organism.

Echinacea dietary supplements are useful adjuvants for live anticoccidial vaccines. Feed supplementation with Echinacea preparations enhances the immune response to such vaccines. Echinacea purpurea in amounts of about 0.1% to 0.5% administered for about two weeks to day-old chicks has been found effective for providing significant weight gain compared to vaccination alone.

Gut health in poultry is improved by providing an efficacious amount of extracts of tannic acid via feed or via drinking water. The extracts of tannic acid are also effective when the poultry are infected with coccidiosis and/or necrotic enteritis. In alternative embodiments, an efficacious amount of Bacillus coagulans is also included.

This invention relates to an organic iodine complex, in which iodine serves as coordination center and functional group-containing organics serve as chelating reagent. The functional groups can be amino and/or hydroxyl and/or carboxyl, and the representatives of hydroxyl- and carboxyl-containing organics are 2-hydroxy-4-methylthiobutanoic acid and 2-hydroxypropanoic acid, respectively. This invention also relates to a combination of organic iodine complex and its application in pharmacy. The combination comprises 0.3~40% organic iodine complex as active ingredient and one or more ingredients chosen from the following ones: natural aminoacyl iodine, solvent, surfactant, food additives, food and synthesized drugs. This combination can be used in preparation of drug against AIDS, white spot syndrome virus in penaeid shrimp, Taura syndrome virus; grass carp hemorrhage virus, stomatitis, pharyngitis, colpitis, tumor, digestive tract diseases in poultry, histomoniasis, coccidiosis, human digestive tract diseases and iodine deficiency diseases.

The invention discloses a Chinese herbal prescription for treating coccidiosis in chicken, which is prepared from sweet wormwood herb, antifeverile dichroa root, rhynohophylla, quisqualis indica, hairyvein agrimony, sanguisorba officinalis, rhizoma atractylodis, coptis, golden cypress, fructus gardeniae praeparatus, andrographis paniculata, philippine violet herb, dried orange peel and liquorice.The prescription has an obvious inhabiting effect on CVCC-245 bacterial strains of Escherichia coli; and coccidiosis ova artificial infection control experiments show that the effective rate is 94.17-99.17%, the field application and popularization tests show that the effective rate is 95.1%, and the application of the prescription has an obvious control effect and safety in use. The prescriptionhas the efficacies of coccidiosis killing, hemostasis, dampness resolving, spleen invigorating and diarrhea relieving with astringents.

The invention belongs to the field of veterinary medicines and particularly relates to a medicine for treating poultry coccidiosis. The medicine comprises, by weight, 2%-8% of sulfaclozine sodium, 0.4%-1.6% of trimethoprim (TMP), 3%-15% of an antifebrile dichroa extractive, 1%-12% of sweet wormwood extractive, 1%-10% of astragalus polysaccharide extractive and the balance an auxiliary material. The medicine is high in safety, and capable of being used for treating the poultry coccidiosis, achieving rapid treatment, preventing recurrence and improving immunity of poultry.

The invention relates to a chicken Eimeria acervulina immunoregulation type DNA vaccine, which belongs to the technical field of biological veterinary medicines. The molecular biology technology is used for connecting lactate dehydrogenase genes of schizont antigen of the first generation of chicken Eimeria acervulina and chIL-2 genes in series to construct a fusion expressing vaccine pVAX-LDH-IL-2. The vaccine comprises a plurality of T cell immune response elements, thus preventing and curing chicken coccidiosis effectively.

The invention discloses a young rabbit feed capable of preventing coccidiosis. The young rabbit feed capable of preventing coccidiosis is characterized by comprising the following raw materials in parts by weight: 300-350 parts of high-quality corns, 100-160 parts of wheat bran, 50-82 parts of bean pulp, 30-52 parts of fish meal, 20-28 parts of bone meal, 8-12 parts of calcium hydrophosphate, 8-11 parts of salt, 55-68 parts of grass meal, 6-9 parts of allicin and 0.5-1 part of diclazuril. The young rabbit feed disclosed by the invention is capable of greatly preventing coccidiosis while providing scientific nutrition for young rabbits.

The invention discloses an anticoccidiosis pharmaceutical composition and a preparation method thereof. The pharmaceutical composition is a traditional Chinese medicine prepared by using fructus cnidii, antifebrile dichroa, radix sophorae flavescentis, radix scutellariae, purslane, hairyvein agrimony, angelica and radix codonopsitis as raw materials. The medicine is used for treating livestock coccidiosis with evident effect, and simultaneously has the advantages of safe use, low production cost, no toxic side-effect and the like.

The invention aims at solving the technical problems that the traditional medicine for treating coccidiosis has a low medicinal curative effect and residues and affects the quality of poultry products. In order to solve the problem, the invention provides a traditional Chinese medicine composition for preventing and controlling poultry coccidiosis and a preparation method of the composition. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 80-160 parts of artemisia apiacea, 80-160 parts of antifebrile dichroa, 60-120 parts of radix bupleuri, 60-120 parts of Chinese pulsatilla root and 60-120 parts of astragalus membranaceus. The traditional Chinese medicine composition can effectively treat and prevent the poultry coccidiosis, is conveniently used, and does not have toxic or side effect and medicine residues after the medicine is admisitered.

The invention discloses a preparation method of an eimeria tenella recombinant polypeptide vaccine VNQS. The preparation method comprises the following steps: preparing raw materials; the method comprises the following steps: 1, cloning a VKVQ encoding gene, designing an upstream primer shown in the formula SEQ ID NO: 3 and a downstream primer shown in the formula SEQ ID NO: 4 according to a chicken coccidiosis gene sequence, carrying out PCR amplification and electrophoresis to obtain a segment of about 1500bp, carrying out ligation transformation on the product to obtain VNQS of which the sequence overall length is 1452bp and of which the sequence is shown in the formula of SEQ ID NO: 1, and carrying out subsequent related steps. The vaccine prepared by the method can effectively controlchicken bodies to be infected with eimeria tenella by immunizing eimeria tenella recombinant polypeptide vaccine VNQS eukaryotic expression plasmids and prokaryotic expression proteins, so that the use amount of anti-coccidiosis drugs in chicken farms is greatly reduced, and chicken coccidiosis is effectively controlled.

The invention discloses an efficient green anti-coccidiosis feed for meat chickens and a preparation method and application thereof. The feed consists of corktree bark, baikal skullcap root, golden thread, dock root, licorice root, garden burnet root, betaine, vitamin A, vitamin K3, calcium hydrogen phosphate, potassium iodide and sodium selenite. The method comprises the following steps of: crushing the Chinese medicaments, sieving the powder with a sieve of 60 meshes, weighing the powder in a weight ratio, mixing for 5 to 10 minutes with an efficient mixer, packing with bags, wherein the mixing uniformity requirement of the mixer comprises that the variation coefficient of the uniformity is not more than 5 percent. The production process for the efficient green anti-coccidiosis feed is simple and easily operated and mastered. The feed is convenient to use and remarkable in effect; the coccidiosis incidence rate of the meat chickens can be reduced to less than 10 percent; and the feed can provide the requirements of the meat chickens on vitamins, trace elements and mineral in the growth and development processes at the same time of improving the coccidiosis resistance of the meat chickens, and has popularization and application value.

The invention relates to the technical field of animal coccidiosis vaccines, in particular to a rabbit coccidiosis live vaccine and a preparation method and application thereof, and provides a rabbitcoccidiosis live vaccine composition which comprises precocious attenuated strains of Eimeria magna, Eimeria intestinalis and Eimeria media and precocious attenuated strain of Eimeria perforans. The rabbit coccidiosis live vaccine has high immunoprotective efficacy and safety, can effectively prevent and treat rabbit coccidiosis and reduce or avoid using of coccidiostats, can remarkably reduce unfavorable impact of vaccine inoculation on growth and production performance like rabbit weight gain and has great economic benefit and wide application prospect. The invention further provides a breeding method of coccidium precocious attenuated strains. The breeding method can effectively shorten breeding period of precocious strains and improve breeding efficiency of the coccidium precocious attenuated strains, obtained precocious strains are stable in hereditary character and high in immunogenicity, and pathogenicity is remarkably lowered.

The invention discloses a pharmaceutical composition for treating chicken coccidiosis. The pharmaceutical composition is prepared from the following components by weight: 0.25 to 2.5 parts of ivermectin, 2.5 to 25 parts of antifeverile dichroa root extract, 15 to 25 parts of astragalus extract, 15 to 25 parts of dark plum extract, 10 to 15 parts of hairyvein agrimony extract and 500 parts of purified attapulgite. The invention also provides a preparation method for the pharmaceutical composition. The invention has the following advantages: the pharmaceutical composition provided by the invention has an anticoccidial index of more than 195 and is composed of both traditional Chinese medicines and western medicines; and an adjuvant material in an oral preparation is attapulgite, so slow release effect is exerted, residues are hardly left, so the pharmaceutical composition has obvious advantages. According to results of in-vitro anticoccidial sporlating test and artificial treatment effect test of chicks infected with coccidia, the traditional Chinese medicine composition provided by the invention has anticoccidial effect; so the composition facilitates further confirmation of the application scope of a compound medicine, provides related enterprises with a novel approach to develop high-quality products, broadens novel application fields of medicine resources, lays a good foundation for research and development of a novel anticoccidial medicine and is of obvious significance.

The invention relates to a rhubarb composition for treating chicken coccidiosis. The composition comprises the following raw materials or extracts of the raw materials or chemically-acceptable derivatives of the raw material extracts; the raw materials include processed products thereof; the composition comprises the following raw materials in part by weight: 10 parts of rhubarb, 3-15 parts of white peony root and 5-15 parts of peach seed, on the basis, and further comprises but is not limited to one or more of 5-18 parts of antifeverile dichroa root, 5-18 parts of sweet wormwood and 3-15 parts of Chinese pulsatilla root. The composition can be prepared into medicinally or pharmaceutically-acceptable physical forms such as powder, liquids, paste, particles, capsules and the like. The composition can be used for treating coccidiosis, relieving pathological injuries caused by coccidian, and lowering economic loss caused by coccidiosis.

The invention discloses a Chicken E.tenella MA2 (Et MA2) gene, a vector thereof, a recombinant strain thereof, a protein thereof, and an application thereof. The amino acid sequence of the EtMA2 protein is represented by SEQ ID No.2. Or the protein is a protein obtained after the amino acid sequence represented by SEQ ID No.2 is subjected to substitution, deletion, and/or addition of one or more amino acids, and/or a protein with terminal modification and with a same activity. The invention also discloses an EtMA2-outsidedomain protein with an amino acid sequence represented by SEQ ID No.4. Or the protein is a protein obtained after the amino acid sequence represented by SEQ ID No.4 is subjected to substitution, deletion, and/or addition of one or more amino acids, and/or a protein with terminal modification and with a same activity. The EtMA2 protein and the EtMA2-outsidedomain protein can be used for preparing eimeria-resisting medicines. Through the immunization of the EtMA2-outsidedomain protein, chicken can be effectively controlled from being infected by E.tenella. Therefore, the dosages of anticoccidial drugs in chicken farms can be greatly reduced, and chicken coccidiosis can be effectively controlled.

The invention discloses an Eimeria tenella gene which is Et STK gene and includes an amino acid sequence shown as SEQ ID NO.1. Moreover, the invention provides a preparation method of a vaccine for prophylaxis and treatment of coccidiosis in chicken. The method includes: connecting the mature peptide of Eimeria tenella conserved protein Et STK with an expression vector of escherichia coli or pichia pastoris cell to obtain a recombinant expression vector, transferring the recombinant expression vector into a host of escherichia coli or pichia pastoris cell to obtain a recombination strain, and cultivating the recombination strain to obtain the vaccine of coccidiosis in chicken. The method has quite high application value.