High Performance Liquid Chromatography Detection Method of Compound Solanum nigrum Anti-inflammatory Tablets
A technology of compound nightshade anti-inflammatory tablets and high-performance liquid chromatography, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems such as the relatively difficult detection method of the active ingredients of compound nightshade anti-inflammatory tablets, and achieve methanol elution capacity The effect of improving, increasing the detection rate, and increasing the absorption peak
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Embodiment 1
[0049] The contents of gallic acid, quercetin, kaempferol, solanine and solanine in compound Solanum nigrum tablets were detected by high performance liquid chromatography (HPLC) with isocratic elution.
[0050] 1. The content of gallic acid, quercetin, and kaempferol in Compound Solanum nigrum Anti-inflammatory Tablets is detected by high-performance liquid chromatography detection method comprising the following steps:
[0051] 1) Chromatographic conditions:
[0052] Chromatographic column: Octadecylsilane bonded silica gel is used as filler;
[0053] Mobile phase: a mixture of methanol and phosphoric acid solution, the volume fraction of phosphoric acid solution is 0.4%, and the volume ratio of methanol and phosphoric acid water is 55:45;
[0054] Flow rate: 1mL / min;
[0055] Column temperature: 25°C;
[0056] Gradient wavelength detection: 0~15min, the detection wavelength is 270nm; 15~30min, the detection wavelength is 362nm;
[0057] 2) Prepare a mixed control soluti...
Embodiment 2
[0073] The contents of gallic acid, quercetin, kaempferol, solanine and solanine in compound Solanum nigrum tablets were detected by gradient elution by high performance liquid chromatography (HPLC).
[0074] 1. The content of gallic acid, quercetin, and kaempferol in Compound Solanum nigrum Anti-inflammatory Tablets is detected by high-performance liquid chromatography detection method comprising the following steps:
[0075] 1) Chromatographic conditions:
[0076] Chromatographic column: Octadecylsilane bonded silica gel is used as filler;
[0077] Mobile phase: Mobile phase A is methanol, mobile phase B is 0.4% phosphoric acid solution, and gradient elution is carried out. The gradient elution procedure is as follows, wherein the mobile phase ratios are all volume percentages:
[0078] 0~13min, mobile phase A: 2%, mobile phase B: 88%;
[0079] 13-14min, mobile phase A: 2%-52%, mobile phase B: 48%-88%;
[0080] 14-30min, mobile phase A: 52%, mobile phase B: 48%;
[0081]...
Embodiment 3
[0105] The contents of gallic acid, quercetin, kaempferol, solanine and solanine in Fufang Solanum xiaoyan tablets were determined by ultra-high performance liquid chromatography (UPLC) with gradient elution.
[0106] The ultra-high performance liquid chromatography principle in this test is basically the same as the high performance liquid chromatography in embodiment 2, and the changed place has the following points:
[0107] (1) Use small particles and high-performance particulate stationary phases. HPLC chromatographic columns, such as: common octadecyl silica gel bonded columns, have a particle size of 5um, while UPLC chromatographic columns reach 3.5um, or even 1.7um, so that the pore size is more conducive to material separation.
[0108] (2) Use of high-pressure infusion pumps. Due to the reduced particle size of the chromatographic column used, the pressure generated during use is naturally multiplied. Therefore, the infusion pump of the liquid chromatography is als...
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