A method for improving chitosan blood compatibility at room temperature
A blood compatibility and chitosan technology, applied in the field of improving chitosan blood compatibility at room temperature, can solve the problems of limited application range, harsh conditions in the synthesis process, and difficulty in preservation, so as to avoid migration orientation, platelet Effects of reduced adhesion and mild conditions
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Embodiment 1
[0035] This embodiment includes the following steps:
[0036] Step 1, under the protection of nitrogen, 16mmol 2-methacryloyloxyethyl phosphorylcholine and 4mmol 2-aminoethyl methacrylate hydrochloride are free under the action of initiator 0.1mmol potassium persulfate Radical polymerization reaction, the temperature of free radical polymerization reaction is 70°C, the time is 12h, dialyze after the end of the reaction, and then freeze-dry at -50°C to obtain phosphorylcholine polymers containing amino groups; The choline polymer is dissolved in methanol (analytical pure) to be prepared as a solution of phosphorylcholine polymer containing amino groups with a concentration of 1 mg / mL;
[0037] Step 2, at room temperature (20° C.), place chitosan in an aqueous glutaraldehyde solution with a mass concentration of 1% and soak for 6 hours, take it out and wash it with distilled water to obtain chitosan grafted with glutaraldehyde;
[0038] Step 3, at room temperature (20 DEG C) co...
Embodiment 2
[0044] This embodiment includes the following steps:
[0045] Step 1, under the protection of nitrogen, 18mmol 2-methacryloyloxyethyl phosphorylcholine and 2mmol 2-aminoethyl methacrylate hydrochloride are free under the action of initiator 0.1mmol potassium persulfate Radical polymerization reaction, the free radical polymerization reaction temperature is 70 ° C, the time is 24 h, after the reaction is completed, dialyzed, and then freeze-dried at -50 ° C to obtain phosphorylcholine polymers containing amino groups; The choline polymer is dissolved in ethanol (analytical pure) to be prepared as a solution of phosphorylcholine polymer containing amino groups with a concentration of 0.5 mg / mL;
[0046] Step 2, at room temperature (30° C.), place the chitosan in an aqueous glutaraldehyde solution with a mass concentration of 2% and soak it for 5 hours, take it out and wash it with distilled water to obtain chitosan grafted with glutaraldehyde;
[0047] Step 3, at room temperatu...
Embodiment 3
[0051] This embodiment includes the following steps:
[0052] Step 1. Under the protection of nitrogen, 16 mmol of acryloyloxyethyl phosphorylcholine and 4 mmol of 2-aminoethyl acrylate hydrochloride are subjected to a free radical polymerization reaction under the effect of initiator 0.2 mmol of potassium persulfate, and the free radical The temperature of the polymerization reaction is 80°C, and the time is 12h. After the reaction is completed, it is dialyzed, and then freeze-dried at -50°C to obtain a phosphorylcholine polymer containing an amino group; the obtained phosphorylcholine polymer containing an amino group is dissolved in In ethanol (analytical pure), be prepared into the solution of the phosphorylcholine polymer that contains amino group that concentration is 5mg / mL;
[0053] Step 2, at room temperature (25° C.), place chitosan in a 0.5% glutaraldehyde aqueous solution and soak for 12 hours, take it out and wash with distilled water to obtain chitosan grafted wi...
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Abstract
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