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Application of eif4g1 in the diagnosis and treatment of squamous cell carcinoma

A 4G1, lung squamous cell carcinoma technology, applied in the field of tumor diagnosis and treatment, can solve the problems of unclear disease mechanism and drug resistance of NSCLC

Active Publication Date: 2020-03-27
SHANGHAI EAST HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional treatment methods (surgery, radiotherapy and chemotherapy) have encountered a bottleneck in improving the five-year survival rate of non-small cell lung cancer. With the advancement of translational medicine research in recent years, some tumor detection methods and targets have been studied, and the development of Some targeted drugs have been released, such as: EGFR mutations and their molecular conformation changes and the clinical application of targeted inhibitors, for specific groups of EGFR sensitive mutations, tyrosine protein kinase inhibitors gefitinib and erlotinib It can significantly prolong the recurrence-free survival of patients with advanced NSCLC, but drug resistance will eventually appear. In the final analysis, the disease mechanism of NSCLC is still unclear

Method used

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  • Application of eif4g1 in the diagnosis and treatment of squamous cell carcinoma
  • Application of eif4g1 in the diagnosis and treatment of squamous cell carcinoma
  • Application of eif4g1 in the diagnosis and treatment of squamous cell carcinoma

Examples

Experimental program
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Effect test

Embodiment 1

[0142] Example 1 Detection of EIF4G1 expression in NSCLC tumor tissue and paracancerous specimens

[0143] Selected 32 cases, all obtained from Shanghai Dongfang Hospital, including different stages of lung adenocarcinoma, squamous cell carcinoma, and large cell carcinoma. The obtained NSCLC cancer and adjacent tissues were detected by Western blot method to detect the expression of EIF4G1 in the tissues of 32 patients, and the relationship between the expression of EIF4G1 and the occurrence and development of NSCLC was analyzed.

[0144] The results showed that among the 32 patients, the expression of EIF4G1 in tumor specimens was higher than that in adjacent tissues in 16 cases (50%) ( figure 1 A), while 12 patients (37.5%) showed the opposite result ( figure 1 C), the expression of EIF4G1 in the paracancerous tissue was higher than that in the tumor tissue; there were 4 patients (12.5%) whose EIF4G1 expression was not significantly different between the tumor specimen and th...

Embodiment 2

[0146] The expression of EIF4G1 in the squamous cell carcinoma case of embodiment 2

[0147] Based on the results of Example 1, further analysis was carried out on the results of EIF4G1 in known squamous cell carcinoma of the lung, the results showed that ( figure 2 ), the expression level of EIF4G1 in all squamous cell carcinomas was higher than that in adjacent tissues, it can be seen that EIF4G1 may be a molecular marker that is generally increased in squamous cell carcinomas.

Embodiment 3

[0148] Example 3 Expansion verification of lung squamous cell carcinoma sample size

[0149] In order to expand the sample size for verification, another 20 cases of tumor tissue and paracancerous tissue known as squamous cell carcinoma (purchased from Xi'an Best Biotechnology Co., Ltd.) were purchased, and the expression of EIF4G1 in squamous cell carcinoma was further analyzed by immunohistochemistry. Express the situation.

[0150] From the results of immunohistochemistry ( image 3 ), in all squamous cell carcinomas, EIF4G1 was higher than that in paracancerous tissues and normal tissues.

[0151] Example 4 Sample Verification of Nasopharyngeal Carcinoma and Breast Cancer Squamous Cell Carcinoma

[0152] Take 20 cases of nasopharyngeal carcinoma and breast cancer (tumor tissue and paracancerous tissue), among which the pathological types of nasopharyngeal carcinoma include squamous cell carcinoma (7 cases) and non-keratinizing carcinoma (13 cases), and the pathological t...

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Abstract

The present invention discloses applications of EIF4G1 in diagnosis of squamous cell carcinomas, particularly uses of a eukaryotic translation initiation factor 4G1 (EIF4G1) gene or protein, or a detection reagent thereof, wherein the eukaryotic translation initiation factor 4G1 (EIF4G1) gene or protein, or detection reagent thereof is used for preparing a kit for determining whether tumors are non-small cell lung squamous cell carcinomas. In addition, experiment results show that EIF4G1 has a certain high expression in squamous cell carcinomas with different sources, such that EIF4G1 can be adopted as the marker of the squamous cell carcinoma pathological type so as to provide the accurate method for typing of different sources of cancers.

Description

technical field [0001] The invention relates to the field of tumor diagnosis and treatment. Specifically, the present invention relates to the application of EIF4G1 gene or its protein, or its detection reagent in the diagnosis and treatment of squamous cell carcinoma. Background technique [0002] Lung cancer is one of the most common malignant tumors in many countries in the world. In my country, lung cancer ranks first in the cause of death from malignant tumors. Lung cancer can be divided into two categories: non-small cell lung cancer (NSCLC) and small cell lung cancer (SCLC), of which non-small cell lung cancer (NSCLC) accounts for 80% to 85% of all lung cancer cases. %, while squamous cell carcinoma is the most common type of lung cancer, accounting for about 50% of the total lung cancer. Only 15-25% of non-small cell lung cancers have the chance of surgical treatment, and even with radical surgery, 50% of NSCLC patients will relapse. Traditional treatment methods ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886G01N33/68G01N33/577G01N33/574A61K39/395A61K48/00A61P35/00
Inventor 徐增光刘亚丽曹月誉袁健李兵马钻邓生琼魏梦丹
Owner SHANGHAI EAST HOSPITAL
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