High-sensitivity and high-specificity fluorescence quantitative PCR detection system and detection method for blood HBV pgRNA

Abstract

The invention discloses a fluorescence quantitative PCR detection system for blood hepatitis B virus (HBV) pregenome RNA (pgRNA) and a method for detecting HBV pgRNA through the detection system.The detection system comprises a forward primer, a reverse primer, a reverse transcription primer with a random anchor sequence and a probe, wherein the nucleotide sequence of the forward primer is shown as SEQ NO:1, the nucleotide sequence of the reverse primer is shown as SEQ NO:2, the nucleotide sequence of the reverse transcription primer is shown as SEQ NO:3, and the nucleotide sequence of the probe is shown as SEQ NO:4.By the adoption of the detection system and the method, an HBV diagnostic reagent or reagent kit can be prepared.According to the detection system and the method, interference possibly brought by HBV DNA or other RNA in the detection process is removed, and the specificity of detection of blood HBV pgRNA is ensured.

Description

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AI Technical Summary

Benefits of technology

This patented technology allows for detection of genetic material from different types of viruses found throughout Japan's mainland regions like Beijing or Taiwan. It also includes various forms that are spread around these areas worldwide.

Problems solved by technology

This patented technical problem addressed by the patant relates to improving the accuracy and precision of measuring viruses like human immunodeficiency Virus type-1 (human immune deficient syndrome), particularly when treatments involve prolong periods between months before symptoms start appearing again after each other's return period from diagnosis. Current methods require expensive equipment and cannot provide real-time results during therapy sessions because they rely heavily upon laborious processes involving complicated steps involved in purification process.

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