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Ganoderma leucocontextum new strain and cultivation method and application thereof

A technology of white-fleshed Ganoderma lucidum and its cultivation method, applied in the field of new strains of white-fleshed Ganoderma lucidum and its cultivation, can solve problems such as no research reports on strains, achieve high nutritional value, good economic and social benefits, and promote splenocyte proliferation Effect

Active Publication Date: 2016-08-17
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The new white-fleshed Ganoderma lucidum strain of the present application is a new strain collected and isolated from the roots of the Pseudomonas ganoderma in the broad-leaved forest of Zhaxigang Township, Bomi County, Nyingchi City. After research, there is no research report on this strain

Method used

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  • Ganoderma leucocontextum new strain and cultivation method and application thereof
  • Ganoderma leucocontextum new strain and cultivation method and application thereof
  • Ganoderma leucocontextum new strain and cultivation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The artificial cultivation method of ganoderma lucidum (CCTCC M 2016087), comprises the following steps:

[0034] 1) According to mass percentage, it is composed of 20% of potatoes, 2% of glucose, 2% of agar, 0.3% of potassium dihydrogen phosphate, 0.15% of magnesium sulfate and trace vitamin B1, and the rest is water. , 121 ℃ high temperature and high pressure damp heat sterilization for 30 minutes;

[0035] 2) Calculated by mass percentage, it is 78% of sorghum, 20% of millet, CaCO 3 2% raw material, water content 60%, make the original seed material, put the original seed material into a 13cm×25cm high temperature resistant transparent polypropylene strain bag, use a small wooden stick to make a hole in the bag material, the hole is as deep as the bag At the bottom, put a plastic ring on the mouth of the bag, and buckle the matching lid to make the original seed bag, which is equivalent to 300g of dry material in each original seed bag;

[0036] 3) By mass percenta...

Embodiment 2

[0044] Example 2 Cell Proliferation Experiment

[0045] 1. Preparation of Chizhi water extract and Ganoderma lucidum water extract

[0046] Crush the commercially available red lucidum and ganoderma ganoderma CCTCC M 2016087 fruit bodies respectively, add distilled water according to the ratio of material to liquid 1:10, keep the temperature in a water bath at 90°C for 3 hours, filter, repeat the extraction of the filter residue for 2 hours, filter, combine the filtrate and concentrate to the material liquid After the ratio is 1:5, add 5 times the volume of absolute ethanol and let it stand for 1 hour, then filter, freeze-dry the precipitate after alcohol precipitation, and obtain the red lucidum water extract and the white-fleshed Ganoderma lucidum water extract respectively.

[0047] 2. Cell culture

[0048] The mice were killed by decapitation, the blood was drained and washed, and soaked in alcohol for 3 minutes for disinfection. Aseptically operate, place the mouse on t...

Embodiment 3

[0063] Embodiment 3 active ingredient content determination

[0064] 1. Extraction and content determination of crude polysaccharide

[0065] Weigh 0.25g red lucidum (commercially available) fruiting body and 0.25g white-fleshed ganoderma CCTCC M 2016087 fruiting body, pulverize, add distilled water according to the ratio of material to water 1:40, bath in boiling water for 2h, centrifuge at 2000g for 15min, add an equal amount of distilled water to boiling water for the residue Bath for 1h, centrifuge at 2000g for 15min, wash the precipitate with 5mL of distilled water, centrifuge at 2000g for 15min, combine the three supernatants, and dilute to 25mL with distilled water. Measure 1.0 mL and add 5 mL of absolute ethanol, oscillate evenly and let stand overnight, centrifuge at 5000 rpm for 15 minutes, discard the supernatant, add 5 mL of absolute ethanol, stand overnight and then centrifuge, dissolve the precipitate with deionized water and dilute to 10mL is the sample solutio...

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Abstract

The invention relates to a Ganoderma leucocontextum new strain and a cultivation method thereof. The Ganoderma leucocontextum new strain is Ganoderma leucocontextum HMGIM-Z110122 of which the preservation number is CCTCC (China Center For Type Culture Collection) NO:M2016087. The cultivation method of the Ganoderma leucocontextum CCTCC NO:M2016087 comprises the following steps: manufacturing a mother strain after the tissue isolation of the strain, manufacturing a stock culture, manufacturing a production strain, and carrying out cultivation and Ganoderma leucocontextum growth management. The Ganoderma leucocontextum new strain and the cultivation method thereof have the beneficial effects that the invention provides the Ganoderma leucocontextum new strain and the corresponding manual cultivation method. Ganoderma leucocontextum is rare at present, can effectively accelerate the proliferation of mouse spleen cells, is high in nutrition value and has good economic benefit and social benefit.

Description

technical field [0001] The invention relates to a new strain of Ganoderma lucidum, in particular to a new strain of Ganoderma lucidum with white flesh and its cultivation method and application. Background technique [0002] Ganoderma (Ganoderma) has a long history of medicinal use in China, and has long been used as an adjuvant treatment for bronchitis, hepatitis, hypertension, tumors, immune disorders and other diseases. Modern pharmacological and clinical research results also show that Ganoderma lucidum contains active ingredients that inhibit tumors and regulate immunity. Since the establishment of Ganodermataceae (Ganodermataceae) by DONK (1948), more than 200 species of Ganodermaceae macrofungi have been reported in the world. There are 103 species of Ganoderma lucidum recorded in my country, 14 of which have been used by people. At present, the varieties widely used in China include Ganoderma lucidum and Ganoderma lucidum, etc. Many other species of Ganoderma lucidu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N5/071C12R1/645A01G1/04A61K36/074A61P37/04
CPCA01G18/00A61K36/074C12N1/14C12N5/0602
Inventor 胡惠萍吴清平谢意珍
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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