Template for allelic standard for dys389 locus

A typing standard and allele technology, applied in the field of forensic medicine, can solve the problems that the flatness of the allelic typing standard cannot be met and the peak heights of difficult-to-amplify fragments are equalized.

CN106350585BActive Publication Date: 2019-06-11INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
0 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2019-06-11

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a standard template for DYS389 locus allelic discrimination. The standard template for DYS389 locus allelic discrimination is a DNA composition, the DNA composition includes a DNA segment 1 - DNA segment 17, the nucleotide sequence is indicated as sequence 1, sequence 2, sequence 3, sequence 4, sequence 5, sequence 6, sequence 7, sequence 8, sequence 9, sequence 10, sequence 11, sequence 12, sequence 13, sequence 14, sequence 15, sequence 16, and sequence 17 in the sequence table. The experiment shows, the template provided by present invention can be augmented to obtain the DYS389 locus allelic discrimination standard, can be used in a variety of commercial kits, and has significant application value.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the technical field of forensic medicine, in particular to a template of an allelic typing standard of the DYS389 gene locus. Background technique

[0002] Humans have 22 pairs of autosomes and 1 pair of sex chromosomes. There are many polymorphic DNA genetic markers on the sex chromosomes. In some special cases (such as sexual assault crimes and partial kinship identification), the analysis of sex chromosome genetic markers has important value. The STR locus of Y chromosome is one of the important genetic markers of Y-DNA. Since the Y chromosome is inherited in linkage and is haplotype, except for the pseudoautochromatic region, it does not recombine with other chromosomes during meiosis classification, so only one allele is detected in most Y-STR loci in a single sample Gene. However, there are a few Y-STR loci that can detect multiple alleles, such as the DYS389 locus, DYS385 locus, DYS527 locus, etc. Among them, the DYS...

Examples

Embodiment 1

[0066] Preparation and verification of the template of the allelic typing standard of embodiment 1, DYS389 locus

[0067] 1. Obtaining recombinant plasmids

[0068] 1. Optimization of the ligation sequence

[0069] hg19 human genome (see website for information on hg19 human genome

[0070] http: / / hgdownload.soe.ucsc.edu / goldenPath / hg19 / bigZips / hg19.2bit ), the DYS389 locus contains the DNA fragment shown in the junction sequence 1, the junction sequence 1:

[0071] 5'-TCATTATACCCTACTTCTGTATCCAACTCTCATCTGTATTATCTATGTA-3'. After a lot of experiments, the connection sequence 1 is optimized to the connection sequence 2:

[0072] 5'-TCTCTTACTATCATGCCATCCTTAAGATTCTTTTCTGTAACTCATAAT-3'. The basic characteristics of junction sequence 1 and junction sequence 2 are shown in Table 1 after evaluation by software OligoAnalyzer 3.1.

[0073] Table 1

[0074]

Number of bases (bp)

GC content (%)

Melting temperature (℃)

ligation sequence 1

48

31.2

...