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Template for allelic standard for dys389 locus

A typing standard and allele technology, applied in the field of forensic medicine, can solve the problems that the flatness of the allelic typing standard cannot be met and the peak heights of difficult-to-amplify fragments are equalized.

Active Publication Date: 2019-06-11
INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] To prepare allelic typing standards, it is necessary to screen blood card samples containing different allelic typing, but the lengths of the two amplified fragments on the DYS389 locus are different and there is an inclusion relationship between them, and the differences in different blood card samples make After amplification, two amplified fragments with different peak heights randomly appear, and it is difficult to level them according to the peak heights of the two amplified fragments, which cannot meet the requirements for the flatness of allelic typing standards

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  • Template for allelic standard for dys389 locus
  • Template for allelic standard for dys389 locus
  • Template for allelic standard for dys389 locus

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Preparation and verification of the template of the allelic typing standard of embodiment 1, DYS389 locus

[0067] 1. Obtaining recombinant plasmids

[0068] 1. Optimization of the ligation sequence

[0069] hg19 human genome (see website for information on hg19 human genome

[0070] http: / / hgdownload.soe.ucsc.edu / goldenPath / hg19 / bigZips / hg19.2bit ), the DYS389 locus contains the DNA fragment shown in the junction sequence 1, the junction sequence 1:

[0071] 5'-TCATTATACCCTACTTCTGTATCCAACTCTCATCTGTATTATCTATGTA-3'. After a lot of experiments, the connection sequence 1 is optimized to the connection sequence 2:

[0072] 5'-TCTCTTACTATCATGCCATCCTTAAGATTCTTTTCTGTAACTCATAAT-3'. The basic characteristics of junction sequence 1 and junction sequence 2 are shown in Table 1 after evaluation by software OligoAnalyzer 3.1.

[0073] Table 1

[0074]

Number of bases (bp)

GC content (%)

Melting temperature (℃)

ligation sequence 1

48

31.2

...

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Abstract

The invention discloses a standard template for DYS389 locus allelic discrimination. The standard template for DYS389 locus allelic discrimination is a DNA composition, the DNA composition includes a DNA segment 1 - DNA segment 17, the nucleotide sequence is indicated as sequence 1, sequence 2, sequence 3, sequence 4, sequence 5, sequence 6, sequence 7, sequence 8, sequence 9, sequence 10, sequence 11, sequence 12, sequence 13, sequence 14, sequence 15, sequence 16, and sequence 17 in the sequence table. The experiment shows, the template provided by present invention can be augmented to obtain the DYS389 locus allelic discrimination standard, can be used in a variety of commercial kits, and has significant application value.

Description

technical field [0001] The invention relates to the technical field of forensic medicine, in particular to a template of an allelic typing standard of the DYS389 gene locus. Background technique [0002] Humans have 22 pairs of autosomes and 1 pair of sex chromosomes. There are many polymorphic DNA genetic markers on the sex chromosomes. In some special cases (such as sexual assault crimes and partial kinship identification), the analysis of sex chromosome genetic markers has important value. The STR locus of Y chromosome is one of the important genetic markers of Y-DNA. Since the Y chromosome is inherited in linkage and is haplotype, except for the pseudoautochromatic region, it does not recombine with other chromosomes during meiosis classification, so only one allele is detected in most Y-STR loci in a single sample Gene. However, there are a few Y-STR loci that can detect multiple alleles, such as the DYS389 locus, DYS385 locus, DYS527 locus, etc. Among them, the DYS...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6858C12Q1/6876C12Q2600/156C12Q2600/166C12Q2531/113C12Q2545/107
Inventor 王乐赵兴春季安全叶健武波
Owner INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY