A kind of leukemia detection kit based on annexin A1 protein

A technology of leukemia and reagent kit, applied in the field of detecting AnnexinA1 protein expression level in subjects

Active Publication Date: 2018-04-13
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no relevant research report on the expression of ANXA1 in leukemia.
At present, there are no relevant research reports on Annexin A1 protein as a kit in the field of leukemia detection

Method used

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  • A kind of leukemia detection kit based on annexin A1 protein
  • A kind of leukemia detection kit based on annexin A1 protein

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0018] 1. Materials and Methods

[0019] 1.1 Cell lines and antibodies

[0020] Human chronic myeloid leukemia cells K562 were purchased from the Tumor Cell Bank of the Chinese Academy of Medical Sciences. The rabbit-derived primary antibody of Annexin A1 protein was purchased from Biotech Biotechnology Co., Ltd., and was diluted at a ratio of 1:1000 when used. The secondary antibody of goat anti-rabbit coupled with horseradish peroxidase was purchased from Biotech Biotechnology Co., Ltd. Co., Ltd., diluted 1:5000 when used. The internal reference antibody GAPDH was also purchased from Biotech and diluted 1:10000 when used.

[0021] 1.2 Leukemia patients and normal blood samples

[0022] Peripheral blood of leukemia patients was obtained from the Department of Hematology, Zhejiang Provincial People's Hospital. Peripheral blood of normal subjects was obtained from the Physical Examination Center of Xiasha District, Zhejiang Provincial Hospital of Traditional Chinese Medicin...

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Abstract

The invention relates to a leukemia detection kit based on AnnexinA1 protein. Specifically, the present invention relates to a method for detection of the expression level of the AnnexinA1 protein in a subject; use of the expression level of the AnnexinA1 protein in the subject in detection of patients with leukemia; and the present invention relates to a kit product comprising an AnnexinA1 protein antibody as an active ingredient and a specification related to the product.

Description

technical field [0001] The invention relates to the development and application of a leukemia detection kit based on Annexin A1 protein. Specifically, the present invention relates to: a method for detecting the expression level of Annexin A1 protein in a subject; the use of the expression level of Annexin A1 protein in a subject in the detection of leukemia patients; and a kit product, which includes as an active Components of Annexin A1 Protein Antibody and Included Instructions pertaining to the product. Background technique [0002] Cancer is a kind of disease that seriously endangers human health. At present, the global cancer death toll is higher than the sum of the death toll from AIDS, tuberculosis and malaria, and it has become the number one killer in the world. On June 2, 2015, the American Conference on Clinical Oncology came to an end. According to the "Global Cancer Report 2014" released by the World Health Organization (WHO), the number of cancer patients and...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): G01N33/577G01N33/68
CPCG01N33/577G01N33/68G01N2800/22G01N2800/50
Inventor付彩云周涵薇陈麒施越葛晨涛
OwnerZHEJIANG SCI-TECH UNIV