Methods of monitoring immunosuppressive therapies in transplant recipient
A technology of immunosuppression and therapy, applied in biochemical equipment and methods, microbiological determination/testing, computer-aided medical procedures, etc., can solve irreplaceable problems
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0090] Example 1 - Analysis of Cell Free DNA to Determine Graft Organ Status in Transplant Recipients and Determine the Need for Modulating Immunosuppressive Therapy
[0091] This example demonstrates the analysis of a sample comprising cell-free DNA from a transplant recipient to determine the level of donor-derived cell-free DNA in the sample. Changes or differences in levels of donor-derived cell-free DNA over time are used to diagnose the status of the transplanted organ in the transplant recipient, as well as to indicate the need for adjustment or maintenance of immunosuppressive therapy administered to the graft recipient.
[0092] object selection
[0093] Human patients who were candidates for kidney transplantation 12 months before the assay described in this example were selected. The patient was being treated with immunosuppressive therapy to prevent allograft rejection. Beginning at the 12th month (one year anniversary) after the transplant event, separate plasma...
Embodiment 2
[0112] Example 2 - Additional Analysis of Cell Free DNA to Determine Graft Organ Status in Transplant Recipients and Determine Need for Modulating Immunosuppressive Therapy
[0113] This example demonstrates that, in addition to the analysis described in Example 1, additional analyzes were performed on a sample comprising cell-free DNA from a transplant recipient to determine the level of donor-derived cell-free DNA in the sample. Changes or differences in levels of donor-derived cell-free DNA over time are used to diagnose the status of the transplanted organ in the transplant recipient, as well as to indicate the need for adjustment or maintenance of immunosuppressive therapy administered to the graft recipient.
[0114] object selection
[0115] Human patients who were candidates for kidney transplantation 12 months before the assay described in this example were selected. The patient was being treated with immunosuppressive therapy to prevent allograft rejection. Beginni...
Embodiment 3
[0135] Example 3 - Analysis of Cell Free DNA to Determine Cardiac Transplant Organ Status in Transplant Recipients and Predict Need for Regulatory Immunosuppressive Therapy
[0136] This example demonstrates the analysis of samples comprising cell-free DNA from 18 transplant recipients to determine the level of donor-derived cell-free DNA in the samples. Levels of donor-derived cell-free DNA over time are used to diagnose the status of a transplanted organ in a transplant recipient and to predict the future status of that transplanted organ, as well as to indicate the need for regulation or maintenance of immunosuppression administered to a graft recipient The need for therapy.
[0137] object selection
[0138] Human patients who were subjects of heart transplantation prior to the assays described in this example were selected. The patient is being treated with immunosuppressive therapy to prevent allograft rejection. During five consecutive months, separate plasma samples...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More