Handheld Electroporation Device

An electroporation, hand-held technology, applied in biochemical equipment and methods, biochemical instruments, methods of stress-stimulating microbial growth, etc. Use quick and efficient effects

Active Publication Date: 2020-07-28
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The traditional electroporation equipment is bulky and inconvenient to operate. The electroporation equipment and the electroporation cup are independent of each other. The electroporation equipment does not have the functions of pipetting and electroporation at the same time.
In the electroporation experiment, it is first necessary to mix the cells and foreign substances in the EP tube, then draw the suspension containing cells and foreign substances into the electroporation cup for electroporation, and then transfer the electroporated suspension Then suck it into the petri dish, the operation is extremely cumbersome

Method used

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  • Handheld Electroporation Device
  • Handheld Electroporation Device
  • Handheld Electroporation Device

Examples

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Embodiment Construction

[0023] Embodiments of the present invention are described in detail below, examples of which are shown in the drawings, wherein the same or similar reference numerals designate the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the figures are exemplary and are intended to explain the present invention and should not be construed as limiting the present invention.

[0024] Refer below Figure 1-Figure 3 A handheld electroporation device 100 according to an embodiment of the present invention is described. The handheld electroporator generally includes: a housing 10 and a gun tip assembly 40 .

[0025] Specifically, such as figure 2 As shown, the housing 10 defines an airtight accommodation chamber, in which an electric pulse unit 20 and a sampling unit 30 are sequentially arranged, and the housing 10 is provided with a mounting hole 11, which communicates with the airtight accommodation c...

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Abstract

A handheld electric drilling apparatus (100) comprising: a housing (10), the housing (10) defining a sealed accommodating cavity, an electric pulse unit (20) and a sampling unit (30) being sequentially provided within the sealed accommodating cavity, the housing (10) being provided with a mounting hole (11), and the mounting hole (11) being in communication with the sealed accommodating cavity; and a head component (40), the head component (40) being connected to the mounting hole (11), the head component (40) comprising a head (41), the head (41) defining a liquid storage cavity (411), the liquid storage cavity (411) being used for storing a suspension containing cells and an exogenous substance, the liquid storage cavity (411) being provided with a gas inlet / outlet (4111) and a liquid inlet / outlet (4112), the sampling unit (30) being connected to the air inlet / outlet (4111), the head component (40) being electrically connected to the electric pulse unit (20), and an electric pulse produced by the electric pulse unit (20) allowing an electric field to be produced in the liquid storage cavity so as to electrically drill the cells and to allow the exogenous substance to be introduced into the cells.

Description

technical field [0001] The invention belongs to the technical field of cell transfection equipment, and in particular relates to a hand-held electroporation device. Background technique [0002] The traditional electroporation equipment is bulky and inconvenient to operate. The electroporation equipment and the electroporation cup are independent of each other. The electroporation equipment does not have the functions of pipetting and electroporation at the same time. In the electroporation experiment, it is first necessary to mix the cells and foreign substances in the EP tube, then draw the suspension containing cells and foreign substances into the electroporation cup for electroporation, and then transfer the electroporated suspension Then suck it into the petri dish, the operation is extremely cumbersome. Contents of the invention [0003] The present invention aims to solve one of the above-mentioned technical problems at least to a certain extent. [0004] For thi...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): C12M1/42
CPCC12M35/02
Inventor徐友春程振苏世圣
OwnerTSINGHUA UNIV