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Method for realizing separation and in-vitro proliferation of stem cells from exfoliated deciduous teeth

A technology of deciduous stem cells and in vitro proliferation, applied in the direction of non-embryonic pluripotent stem cells, animal cells, vertebrate cells, etc., can solve the problems involved in the research and utilization of embryonic stem cells, and achieve the effect of a broad space

Inactive Publication Date: 2017-10-03
北京泰盛生物科技有限公司
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Problems solved by technology

However, the research and utilization of embryonic stem cells involves certain ethical issues

Method used

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  • Method for realizing separation and in-vitro proliferation of stem cells from exfoliated deciduous teeth
  • Method for realizing separation and in-vitro proliferation of stem cells from exfoliated deciduous teeth
  • Method for realizing separation and in-vitro proliferation of stem cells from exfoliated deciduous teeth

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Embodiment Construction

[0028] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Apparently, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0029] refer to figure 1 The method for separating and in vitro proliferation of exfoliated deciduous teeth stem cells includes the following steps: selecting and collecting deciduous teeth 1 extracted from healthy children aged 6-10 due to retention and without dental pulp disease. Immediately after pulling it out, put it into the test tube 3 containing the PBS reagent 2 and store it for later use, and collect the material within 4 hours. Then take out the deciduous tooth 1...

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Abstract

The invention discloses a method for realizing separation and in-vitro proliferation of stem cells from exfoliated deciduous teeth. The method comprises the steps of tooth selection, deciduous tooth cutting, separation of the stem cells from the deciduous teeth, culture and proliferation of the stem cells from the deciduous teeth, and the like. The method has the beneficial effects that the purified stem cells from the human exfoliated deciduous teeth through the method can differentiate towards the specific cell types including osteoblasts, lipoblasts, neurocytes and the like under certain conditions, and thus wide space is provided for researching dental pulp stem cells.

Description

technical field [0001] The present invention relates to the technical field of stem cells, in particular to a method for separating and in vitro proliferation of stem cells from exfoliated deciduous teeth. The stem cells from deciduous human exfoliated teeth are separated, and then the stem cells from deciduous human exfoliated deciduous teeth are cloned and purified by the limited dilution method, and the stem cells from deciduous human exfoliated teeth are proliferated. . Background technique [0002] Stem cells are early undifferentiated cells with multi-differentiation potential and self-replication function. According to the cell source, stem cells can be divided into embryonic stem cells and adult stem cells. Embryonic stem cells are the most primitive stem cells, which are located at the top of individual development, can differentiate into all types of cells that make up the body, and then form any tissue and organ of the body, with developmental totipotency. Howev...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/074
CPCC12N5/0664
Inventor 张建国周彦恒
Owner 北京泰盛生物科技有限公司
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