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A compound pharmaceutical composition with anti-lung cancer effect and application thereof

A composition and anti-lung cancer technology, applied in the field of natural medicine, to achieve the effects of reducing toxic and side effects, strong anti-proliferation activity, and good prospects for clinical drug development

Active Publication Date: 2020-07-28
佛山市第五人民医院 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Based on the above background, after literature research, the combination of PLM and ED for the prevention and treatment of malignant tumor lung cancer has not been reported in the relevant literature.

Method used

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  • A compound pharmaceutical composition with anti-lung cancer effect and application thereof
  • A compound pharmaceutical composition with anti-lung cancer effect and application thereof
  • A compound pharmaceutical composition with anti-lung cancer effect and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: Screening and optimizing the compound pharmaceutical composition of perylene amide (PLM) and emodin (ED) in lung cancer cells.

[0019] Lung cancer cells in the logarithmic growth phase were inoculated with 3×10 4 Cells / well were placed on a 96-well plate, and after 6 hours of growth, the supernatant was discarded by centrifugation, and then administered according to the following groups: Tumor cells were divided into no drug group and drug-added group, and the drug-added group consisted of ED and PLM single drugs Group, ED and PLM combined drug groups with different molar ratios, set 4-6 duplicate wells in each group, culture for 24 hours, discard the supernatant, add 100 μl of MTT (tetrazolium salt) serum-free culture medium containing 0.5 mg / ml for culture After 4 hours, add 100 μl of DMSO (dimethyl sulfoxide), place on a micro-oscillator to vibrate for 10 minutes, and then place on a microplate reader to detect the OD value at 570 nm. Results The inhibit...

Embodiment 2

[0025] Example 2: Analysis of the combined administration effect of PLM and ED

[0026] Based on the Median-effect Principle (moderate effect principle or Chou-Talalay combined index method), the dose-response curve and the combined index curve (fa-C curve) under different effects were drawn using CombiDrug statistical software. The relationship between the effect and the combination index quantitatively evaluates whether there is a synergistic, antagonistic or additive relationship between the two drugs. Specific steps are as follows:

[0027] The drug effect is the inhibition rate (fa)=1-(the average OD570 value of the test group / the average OD570 of the tumor cell blank control group) according to the intermediate effect equation fa / fu=(D / Dm) m , take the logarithm logfa / fu=mlogD–mlogDm on both sides, set a=-mlogDm, b=m, x=logD, y=logfa / fu, and substitute into the intermediate effect equation to get y=bx–a; where fa is the drug effect , fu=1–fa, D is the drug concentratio...

Embodiment 3

[0031] Embodiment 3: Pilongamide (PLM) and emodin (ED) compound pharmaceutical composition are made tablet according to following prescription:

[0032] The prescription is as follows:

[0033]

[0034] The preparation process is as follows:

[0035] Pass the medicine and auxiliary materials through a 80-mesh sieve respectively, fully mix the perylene amide with 50 grams of microcrystalline cellulose and 12 grams of carboxymethyl starch sodium, make soft materials with 10% starch slurry, granulate with a 18-mesh sieve, and dry at 60°C , get particle 1. Fully mix emodin with 12 grams of microcrystalline cellulose, 15 grams of starch and 8 grams of sodium carboxymethyl starch, make soft material with 10% starch slurry, granulate with a 18-mesh sieve, and dry at 60°C to obtain Granule 2. According to the principle of equal increase, fully mix granule 1 and granule 2, sieve the granules with 16 mesh, add magnesium stearate, mix well, and compress into tablets, the tablet weight...

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Abstract

The invention belongs to the field of natural medicine, and discloses a compound medicine composition with a lung cancer resistant effect and application of the compound medicine composition. Active ingredients of the compound medicine composition consist of piperlongumine and rheum emodin in a molar ratio of (1:1) to (1:10). By combining the piperlongumine and rheum emodin, a synergic effect for treating the lung cancer is relatively good.

Description

technical field [0001] The invention belongs to the field of natural medicines, in particular to a compound pharmaceutical composition containing pinionamide and emodin with anti-lung cancer effect and its application. Background technique [0002] Lung cancer is one of the malignant tumors with the fastest increasing morbidity and mortality and the greatest threat to the health and life of the population. In the past 50 years, many countries have reported that the morbidity and mortality of lung cancer have increased significantly. The incidence and mortality of lung cancer in men rank first among all malignant tumors, and the incidence and mortality of lung cancer occupy the second place in women. The cause of lung cancer is still not fully understood. At present, the common treatment methods for lung cancer include: surgery, radiotherapy, chemotherapy, and traditional Chinese medicine. Among them, surgery and radiotherapy are the most commonly used methods, but surgical...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/4412A61K31/122A61P35/00
CPCA61K31/122A61K31/4412A61K2300/00
Inventor 陈钢黄文柱王志军严文刘连刘腾彭咏波
Owner 佛山市第五人民医院
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