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A compound pharmaceutical composition with anti-lymphoma effect and application thereof

A composition and lymphoma technology, applied in the field of natural medicine, to achieve the effects of reducing toxic and side effects, good clinical drug development prospects, and strong anti-proliferation activity

Active Publication Date: 2019-05-21
FOSHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Combination of houttuyfotin and natural drug peronylamide for the prevention and treatment of malignant tumor lymphoma has not been seen. There are almost no relevant literature reports.

Method used

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  • A compound pharmaceutical composition with anti-lymphoma effect and application thereof
  • A compound pharmaceutical composition with anti-lymphoma effect and application thereof
  • A compound pharmaceutical composition with anti-lymphoma effect and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Daudi, Raji and BC-3 cells screened and optimized the compound pharmaceutical composition of perylene amide (PLM) and houttuydine (DLH).

[0018] Take the cells in the logarithmic growth phase and inoculate 3×10 4 Cells / well were placed on a 96-well plate, and after 6 hours of growth, the supernatant was discarded by centrifugation, and then administered according to the following groups: Tumor cells were divided into no-drug group and drug-added group, and DLH and PLM were used in the drug-added group. Group, DLH and PLM combined drug groups with different molar ratios, set 4-6 duplicate wells in each group, culture for 24 hours, discard the supernatant, add 100 μl DLHT (tetrazolium salt) serum-free culture medium containing 5 mg / ml and culture for 4 After 1 hour, add 100 μl of DMSO (dimethyl sulfoxide), place on a micro-oscillator and vibrate for 10 minutes, and then place on a microplate reader to detect the OD value at 570 nm. Results The inhibition rate...

Embodiment 2

[0024] Example 2: Analysis of the combined administration effect of DLH and PLM

[0025] Based on the Median-effect Principle (moderate effect principle or Chou-Talalay combined index method), the dose-response curve and the combined index curve (fa-C curve) under different effects were drawn using CombiDrug statistical software. The relationship between the effect and the combination index quantitatively evaluates whether there is a synergistic, antagonistic or additive relationship between the two drugs. Specific steps are as follows:

[0026] The drug effect is the inhibition rate (fa)=1-(the average OD570 value of the test group / the average OD570 of the tumor cell blank control group) according to the intermediate effect equation fa / fu=(D / Dm) m , take the logarithm logfa / fu=mlogD–mlogDm on both sides, set a=-mlogDm, b=m, x=logD, y=logfa / fu, and substitute into the intermediate effect equation to get y=bx–a; where fa is the drug effect , fu=1–fa, D is the drug concentrati...

Embodiment 3

[0031] Embodiment 3: Pilongamide (PLM) and Houttuyfonate (DLH) compound pharmaceutical composition is made into tablet according to the following prescription:

[0032] The prescription is as follows:

[0033]

[0034] The preparation process is as follows:

[0035] The medicine and auxiliary materials are passed through 80 mesh sieves respectively, fully mixed with 48 grams of microcrystalline cellulose and 12 grams of carboxymethyl starch sodium, 10% starch slurry, granulated with 18 mesh sieves, and dried at 60°C , get particle 1. Houttuyfondin was thoroughly mixed with 24 grams of microcrystalline cellulose, 15 grams of starch and 8 grams of sodium carboxymethyl starch, 10% starch slurry was made into soft material, granulated with a 18-mesh sieve, and dried at 60°C to obtain Granule 2. According to the principle of equal increase, fully mix granule 1 and granule 2, sieve the granules with 16 mesh, add magnesium stearate, mix well, and compress into tablets, the tablet ...

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Abstract

The invention belongs to the field of natural medicines, and discloses a compound medicinal composition containing perylene amide and houttuyonin and having an anti-lymphoma effect and its application. The active ingredient of the compound pharmaceutical composition is composed of perylene amide and houttuyonin in a molar ratio of 1:1 to 1:20. The combination of the two drugs has a strong synergistic effect on treating lymphoma.

Description

technical field [0001] The invention belongs to the field of natural medicines, and in particular relates to a compound pharmaceutical composition containing perylene amide and houttuyonin with anti-lymphoma effect and its application. Background technique [0002] Cancer is a major chronic disease that seriously endangers human health, and has become the second largest killer after cardiovascular disease. Since the beginning of the 21st century, due to the rapid process of modern industrialization, environmental pollution and many other factors, the immune system of lymphocytes has been destroyed and the incidence of lymphoma has been increasing year by year. The drug system is very urgent. [0003] Piperlongumine (PLM), also known as piperlongumine (piplOMAine), belongs to alkaloid compounds. It was originally isolated from the roots of Piper longum Linn., a plant of the family Piperaceae, and it was also obtained from the roots of P. sylvaticum Roxb. and P. tuberculatum...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/4412A61K31/121A61P35/00
CPCA61K31/121A61K31/4412A61K2300/00
Inventor 刘连刘腾彭咏波邱桥春
Owner FOSHAN UNIVERSITY
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