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Method for studying interaction between eggplant extract and bovine serum albumin by fluorescence spectroscopy

A technology of bovine serum albumin and fluorescence spectroscopy, which is applied in the field of fluorescence spectroscopy to study the interaction between eggplant extract and bovine serum albumin

Inactive Publication Date: 2018-04-20
XINYANG NORMAL UNIVERSITY
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, there have been many studies on the interaction between various natural products and bovine serum albumin, and more in-depth discussions on quenching methods, binding sites, and interaction forces. Solvent extraction of flavonoids from different parts of eggplant, so as to explore the changes in the fluorescence spectra of diff

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  • Method for studying interaction between eggplant extract and bovine serum albumin by fluorescence spectroscopy
  • Method for studying interaction between eggplant extract and bovine serum albumin by fluorescence spectroscopy
  • Method for studying interaction between eggplant extract and bovine serum albumin by fluorescence spectroscopy

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Embodiment 1

[0034] Experimental part of embodiment 1

[0035] 1.1 Experimental materials

[0036] 1.1.1 Instruments and reagents

[0037] Cary Eclipse fluorescence spectrophotometer (Varian, USA); HH-2 digital constant temperature water bath (Jintan Jerry Electric Co., Ltd.; electronic analytical balance (Shanghai Yueping Scientific Instrument Co., Ltd.); pHSJ-3F type Acidity meter (Shanghai Leici Instrument Factory)

[0038] Bovine serum albumin (biochemical reagent, molecular weight 66000); Tris(hydroxymethyl)aminomethane (analytical pure); Dehydrated alcohol (analytical pure); Methanol (analytical pure); Purple eggplant (purchased in Tanshan cabbage market); experiment Water is double distilled water

[0039] 1.1.2 Preparation of solution

[0040]Preparation of 0.1mol / L pH=7.4 Tris-HCl buffer solution: Accurately weigh 3.0285g of tris(hydroxymethyl)aminomethane solid Tris in a 250mL volumetric flask, dissolve it in water and set the volume to 250mL, and prepare 0.1mol / L Tris solut...

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Abstract

The invention relates to a method for studying the interaction between eggplant extract and bovine serum albumin by fluorescence spectroscopy. Flavonoid compounds are extracted from different parts ofeggplants by a refluxing technology under different solvent conditions, and are analyzed by the fluorescence spectroscopy, the interaction between the eggplant extract and the bovine serum albumin isstudied at a pH value being 7.40 and being close to the physiological environment of human bodies, and the influences of the eggplant extracts of the different parts of and the type of solvents on the fluorescence intensity are discussed. Experimental results show that when methanol is used as a solvent, each of the extracts of eggplant stems, eggplant skins and eggplant pulp quenches the fluorescence of BSA, and the maximum fluorescence emission wavelength has no obvious red shift; and when ethanol is used as a solvent, each of the extracts of eggplant stems, eggplant skins and eggplant pulpquenches the fluorescence of the BSA, and the maximum fluorescence emission wavelength is significantly red-shifted. The fluorescence value of the interaction between the eggplant methanol extractand the BSA and the fluorescence values of the interaction between the eggplant ethanol extract and the BSA decrease with the increase of the extract concentration, and the decrease ways and the decrease degrees are different.

Description

technical field [0001] The invention belongs to the technical field of fluorescence spectroscopy, in particular to a method for studying the interaction between eggplant extract and bovine serum albumin by fluorescence spectroscopy. Background technique [0002] Eggplant is the fruit of the Solanaceae plant, which is a herbaceous plant. Originally produced in the tropics, it was later introduced to China. The history of people planting eggplant can be traced back to more than 2000 years ago. The phenomenon of planting eggplant in various parts of our country is very common. It is one of the common foods that people like most. It can not only prevent and treat many diseases, but also has high medicinal value. It is a cheap and affordable vegetable. Eggplant is rich in protein, vitamin P, vitamin C, trace elements, sugars and alkaloids, etc. Eggplant is a cold food, and it has a good effect in summer. It has the effects of dispelling wind, dredging collaterals, reducing swel...

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/64G01N2021/6417
Inventor 吕艳阳宋丽冉
Owner XINYANG NORMAL UNIVERSITY
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