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Composition and application of composition to protection of HK-2 cells damaged by calcium oxalate monohydrate

A kind of technology of HK-2 and composition, applied in the application field of HK-2 cells, can solve the problems such as no report on medicinal components

Inactive Publication Date: 2018-11-16
GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on Desmodium sativa is limited to the research on the pharmacodynamic effect of the total flavonoids, and the research on the medicinal components has not been reported yet.

Method used

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  • Composition and application of composition to protection of HK-2 cells damaged by calcium oxalate monohydrate
  • Composition and application of composition to protection of HK-2 cells damaged by calcium oxalate monohydrate
  • Composition and application of composition to protection of HK-2 cells damaged by calcium oxalate monohydrate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0158] The present embodiment provides a composition, and the composition mainly includes the following components by mass percentage:

[0159] 5% Weitsanin-1, 8% Weitsanin-2, 8% Weitsanin-3, 15% schaftosides, 23% isoschaftosides and the rest of the cell culture medium.

Embodiment 2

[0161] The present embodiment provides a composition, and the composition mainly includes the following components by mass percentage:

[0162] 11% Weitsanin-1, 15% Weitsanin-2, 15% Weitsanin-3, 30% Schaftoside, 25% Isoschaftaside and the rest of the cell culture medium.

[0163] Compositions provided in Example 1 and Example 2 were used to culture and detect COM-injured HK-2 cells using the method provided in the test example. It was found that the compositions provided in Example 1 and Example 2 could reduce the damage of mitochondrial membrane potential in HK-2 cells stimulated by COM, up-regulate the protein expression of occludin and ZO-1, and reduce cell damage and apoptosis.

[0164] It shows that the compositions provided in Example 1 and Example 2 have the effect of protecting HK-2 cells damaged by calcium oxalate monohydrate.

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Abstract

The invention relates to the field of medicines, in particular to composition and an application of the composition to protection of HK-2 cells damaged by calcium oxalate monohydrate. The compositionis mainly prepared from following components in percentage by mass: 5%-11% of Vicenin-1, 8%-15% of Vicenin-2, 8%-15% of Vicenin-3, 15%-60% of Schaftoside and 23%-45% of Schaftoside. By means of cooperation of five compounds, the composition in the embodiment of the invention protects the HK-2 cells damaged by calcium oxalate monohydrate and further can be used for preparing medicines for treatingkidney stone. The composition can be combined with specific sites on the HK-2 cells, and is an effective component for playing the pharmaceutical effect.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a composition and its application in protecting HK-2 cells damaged by calcium oxalate monohydrate. Background technique [0002] Desmodium styracifolium (Osb.) Merr. is the dry above-ground part of Desmodium styracifolium (Osb.) Merr. in the leguminous family. The medicinal name comes from "Lingnan Herbal Records". Slightly cold in nature, sweet and light in taste, it belongs to the bladder, liver, gallbladder, and kidney channels, and has the effects of inducing diuresis to relieve stranguria, clearing heat and dehumidification, and removing stones. Clinically, it is mainly used for urinary tract infection, urinary calculus, cholelithiasis, acute icteric hepatitis, etc., and has a good curative effect. At present, the research on Desmodium sativa is limited to the research on the pharmacodynamic effect based on the total flavonoids, and the research on the medicinal components has not b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61P13/12
Inventor 陈丰连林裕英李明慧
Owner GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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