Caps markers related to wheat seed dormancy/ear germination resistance and its detection method

Abstract

The invention discloses a CAPS marker related to wheat seed dormancy / ear germination resistance and a detection method thereof, and relates to the technical field of wheat molecular breeding. The CAPS marker is named as Qsd1‑5BL, the nucleoside of Qsd1‑5BL The acid sequence is SEQ ID NO.1, and the present invention also provides a primer pair for amplifying the above-mentioned CAPS marker, and a method for identifying the duration of wheat seed dormancy / ear germination resistance using the above-mentioned CAPS marker. After performing PCR amplification on the wheat DNA, use the HhaI restriction endonuclease to perform digestion to obtain the digestion product; when the digestion product is a main band, the corresponding wheat seeds have a longer seed dormancy period or a longer Ear germination resistance duration; when the enzyme digestion product is two main bands, the corresponding wheat seeds have a shorter seed dormancy period or a shorter ear germination resistance duration, and the CAPS marker provided by the invention can be used for cultivating Wheat cultivars with strong ear germination resistance provide genetic resources and functional markers to broaden their resistance genetic basis.

Description

technical field [0001] The invention relates to the technical field of wheat molecular breeding, in particular to a gene related to wheat seed dormancy or ear germination resistance and a detection method thereof. Background technique [0002] Wheat is one of the most important food crops in the world, providing about 21% of food calories and 20% of protein for humans. China is the world's largest wheat producer and consumer. Therefore, high yield, stable yield and high quality have always been important goals of wheat breeding. However, if the wheat harvest period encounters rainy weather, it is easy to germinate on the ear (abbreviated as ear germination). If the rainy weather lasts for a long time, it will intensify the ear germination and seriously affect the yield and quality. Therefore, cultivating wheat varieties with long duration of resistance to panicle germination is particularly necessary, which is the fundamental way to reduce its damage; cloning genes with lon...

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Problems solved by technology

However, previous studies mostly focused on the effect of seed dormancy depth in a certain period of time after harvest on ear germination resistance, ignoring the fact that longer seed dormancy periods are resistant to ear germination persistence in the environment of longer duration of rainy wheat harvest season. the role of

Therefore, there are many reports on the depth (or level) of seed dormancy, such as the cloned Tasdr (2As / 2Bs, Zhang et al. 2014, 2017), TaVp-1 (3AL / 3BL, yang et al. 2007; Chang et al. 2011), TaMFT and TaPHS1 (3As, Nakamura et al. 2011; Liu et al. 2013) and PM19-A1 (4AL, Barrero et al. 2015) and TaMKK3-A (4AL, Torada et al. 2016); genes that regulate the duration of seed dormancy (referred to as dormancy) Fewer reports have hindered the cultivation of varieties with longer duration of panicle germination resistance to a certain extent

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