A caps marker related to wheat ear germination resistance/sensitivity and its application

A technology of ear germination and wheat, applied in the field of CAPS markers and applications, can solve the problems affecting the efficiency and accuracy of molecular marker-assisted selection breeding, and achieve the effect of broadening the genetic basis of resistance

Active Publication Date: 2021-10-26
ANHUI AGRICULTURAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

Previous studies have used linkage analysis and association analysis to locate a large number of QTLs related to wheat seed dormancy / ear germination resistance. However, these QTLs are not functional markers, but only represent a certain segment on the chromosome, which affects molecular marker-assisted selection breeding. efficiency and accuracy

Method used

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  • A caps marker related to wheat ear germination resistance/sensitivity and its application
  • A caps marker related to wheat ear germination resistance/sensitivity and its application
  • A caps marker related to wheat ear germination resistance/sensitivity and its application

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Embodiment Construction

[0024] The methods used in this example are conventional methods known to those skilled in the art unless otherwise specified. The reagents and other materials used are all commercially available products unless otherwise specified. The germplasm resources used can all be obtained from national species The quality resource library is obtained.

[0025] (1) Determination of wheat seed dormancy phenotype

[0026] (1) Determination of seed germination index (GI)

[0027] The main stem ears were harvested at the wax ripening stage of wheat, air-dried indoors for 2 days, and immediately stored in a refrigerator at -20°C to maintain the dormancy of the seeds. After all the test materials were harvested, they were hand-threshed and post-ripened for 5 days and 15 days. and a 30-day grain germination test. Specifically: take 50 grains of each material for seed germination test, repeat twice. Put the grains in a petri dish with the ventral groove facing down, add 9ml of sterile water...

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Abstract

The invention discloses a CAPS marker related to wheat ear germination resistance / sensitivity and its application, and relates to the technical field of genetic breeding. The invention discloses a candidate gene for controlling wheat seed dormancy / ear germination resistance. The gene is named TaGASR‑7B, and the nucleotide sequence of the TaGASR‑7B is specifically SEQ ID NO.1, and a CAPS marker was developed based on the candidate gene, and the sequence listing of the CAPS marker is SEQ ID NO.4, A method for identifying wheat ear germination resistance or susceptibility using the CAPS marker, using a primer pair to amplify the CAPS marker and then digesting it. If the digested product has two main bands, the gene sequence corresponding to the product is Named as GS7B1‑R, the corresponding wheat variety exhibits ear germination resistance; if the enzyme digestion product is a main band, the gene sequence corresponding to the product is named GS7B1‑S, and the corresponding wheat variety exhibits ear germination resistance ; The present invention can provide genetic resources and functional markers for wheat ear germination resistance breeding, and broaden its resistance genetic basis.

Description

technical field [0001] The invention relates to the technical field of crop genetics and breeding, in particular to a CAPS marker related to wheat ear germination resistance or susceptibility and its application. Background technique [0002] Wheat is the staple food of about 40% of the world's population. my country is the world's largest wheat producer and consumer, accounting for 17% of the world's total wheat production and 16% of its total consumption. Pre-harvest sprouting (PHS) refers to the phenomenon that wheat grains germinate directly on the ear due to rainy weather just before harvest. Ear germination of wheat will seriously reduce the yield and quality, and even threaten the food security of our country and the world. [0003] Seed dormancy is the main genetic factor for ear germination resistance. Previous studies have used linkage analysis and association analysis to locate a large number of QTLs related to wheat seed dormancy / ear germination resistance. How...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/11C12Q1/6895
CPCC07K14/415C12Q1/6895C12Q2600/13C12Q2600/158
Inventor 张海萍常成王升星朱玉磊姜昊曹佳佳李黎未文新卢杰陈璨司红起马传喜
Owner ANHUI AGRICULTURAL UNIVERSITY
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