CAPS marker relevant to ear sprouting resistance/sensibility of wheat and applications of CAPS marker

A technique for panicle germination and marking, applied in the field of CAPS marking and application, can solve the problems affecting the efficiency and accuracy of molecular marker-assisted selection breeding, and achieve the effect of broadening the genetic basis of resistance

Active Publication Date: 2018-11-30
ANHUI AGRICULTURAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

Previous studies have used linkage analysis and association analysis to locate a large number of QTLs related to wheat seed dormancy / ear germination resistance. However, these QTLs are not functional markers, but only represent a certain segment on the chromosome, which affects molecular marker-assisted selection breeding. efficiency and accuracy

Method used

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  • CAPS marker relevant to ear sprouting resistance/sensibility of wheat and applications of CAPS marker
  • CAPS marker relevant to ear sprouting resistance/sensibility of wheat and applications of CAPS marker
  • CAPS marker relevant to ear sprouting resistance/sensibility of wheat and applications of CAPS marker

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Embodiment Construction

[0024] The methods used in this example are conventional methods known to those skilled in the art unless otherwise specified. The reagents and other materials used are all commercially available products unless otherwise specified. The germplasm resources used can all be obtained from national species The quality resource library is obtained.

[0025] (1) Determination of wheat seed dormancy phenotype

[0026] (1) Determination of seed germination index (GI)

[0027] The main stem ears were harvested at the wax ripening stage of wheat, air-dried indoors for 2 days, and immediately stored in a refrigerator at -20°C to maintain the dormancy of the seeds. After all the test materials were harvested, they were hand-threshed and post-ripened for 5 days and 15 days. and a 30-day grain germination test. Specifically: take 50 grains of each material for seed germination test, repeat twice. Put the grains in a petri dish with the ventral groove facing down, add 9ml of sterile water...

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Abstract

The invention discloses a CAPS marker relevant to ear sprouting resistance/sensibility of wheat and applications of the CAPS marker, and relates to the technical field of genetic breeding. The invention discloses a candidate gene for controlling the seed dormancy/ear sprouting resistance of wheat, the candidate gene is named as TaGASR-7B, the nucleotide sequence of TaGASR-7B is specifically SEQ IDNO.1, meanwhile, one CAPS marker is developed according to the candidate gene, and a sequence table of the CAPS marker is SEQ ID NO.4. For a method for identifying the ear sprouting resistance or sensibility of the wheat adopting the CAPS marker, primer pairs are adopted for amplifying the CAPS marker, digestion is then carried out, if the digestion product is two main bands, the gene sequence corresponding to the product is named as GS7B1-R, and the corresponding wheat variety shows ear sprouting resistance; and if the digestion product is one main band, the gene sequence corresponding to the product is name as GS7B1-S, and the corresponding wheat variety shows ear sprouting sensibility. With the adoption of the technical scheme, a gene resource and a functional marker are provided for ear-sprouting-resistant breeding of wheat, and thus the resistance hereditary basis of wheat is widened.

Description

technical field [0001] The invention relates to the technical field of crop genetics and breeding, in particular to a CAPS marker related to wheat ear germination resistance or susceptibility and its application. Background technique [0002] Wheat is the staple food of about 40% of the world's population. my country is the world's largest wheat producer and consumer, accounting for 17% of the world's total wheat production and 16% of its total consumption. Pre-harvest sprouting (PHS) refers to the phenomenon that wheat grains germinate directly on the ear due to rainy weather just before harvest. Ear germination of wheat will seriously reduce the yield and quality, and even threaten the food security of our country and the world. [0003] Seed dormancy is the main genetic factor for ear germination resistance. Previous studies have used linkage analysis and association analysis to locate a large number of QTLs related to wheat seed dormancy / ear germination resistance. How...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/11C12Q1/6895
CPCC07K14/415C12Q1/6895C12Q2600/13C12Q2600/158
Inventor 张海萍常成王升星朱玉磊姜昊曹佳佳李黎未文新卢杰陈璨司红起马传喜
Owner ANHUI AGRICULTURAL UNIVERSITY
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