Wheat seed dormancy duration gene TaCNGC-2A and functional marker thereof

Abstract

The invention belongs to the field of crop genetics and breeding, and provides a wheat dormancy duration candidate gene TaCNGC-2A. A wheat plant pre-harvest sprouting resistance duration judgment functional marker, namely CAPS marker CNG2A, is obtained by using the candidate gene, the functional marker has A/T base variation at the TaCNGC-2A gene promoter-313 position, and the marker sequence is shown as SEQ ID NO.2. A corresponding marker primer is designed according to the marker sequence, and the length of an amplification product is 813 bp. The amplification product can be directly detected in 1.5% agarose gel electrophoresis after being digested at 37 DEG C for 3 h by a FokI restriction enzyme (GGATGN9/). The CNG2A-a allelic variation cannot be digested, while the CNG2A-b allelic variation can be digested, and the digestion products are 584 bp and 229 bp respectively; besides, plants carrying the CNG2A-b allelic variation have a longer pre-harvest sprouting resistance duration than plants carrying CNG2A-a. The marker can be directly applied to molecular-marker-assisted wheat pre-harvest sprouting resistance breeding selection and also can accelerate the cultivation process ofpre-harvest sprouting resistant varieties.

Description

technical field [0001] The invention belongs to the field of crop genetics and breeding, and relates to a wheat seed dormancy duration gene TaCNGC-2A and a functional marker thereof. Background technique [0002] About 35-40% of the world's population uses wheat as a staple food, and China is the world's largest wheat producer and consumer. Therefore, high yield, stable yield and high quality have always been important goals of wheat breeding (He Zhonghu et al. 2018). However, if the wheat harvest period encounters rainy weather, it is easy to germinate on the ear (abbreviated as ear germination). If the rainy weather lasts for a long time, it will intensify the ear germination and seriously affect the yield and quality. [0003] Seed dormancy is the main genetic factor of ear germination resistance, which includes two aspects of dormancy depth (level) and duration. Seed dormancy degree deep (or high level) and long duration have stronger resistance to ear germination. Howe...

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Problems solved by technology

However, previous studies mostly focused on the effect of seed dormancy depth on ear germination resistance at a certain period after harvest, ignoring the long duration of seed dormancy in the environment of longer duration of rainy wheat harvest season. The role of sex

Therefore, there are many QTL / gene reports on the depth of seed dormancy, such as the cloned TaSdr (2AS / 2BS, Zhang et al. 2014, 2017), TaVp-1 (3AL / 3BL, Yang et al. 2007; Chang et al. 2011), TaMFT and TaPHS1 (3AS, Nakamura et al. 2011; Liu et al. 2013), PM19-A1 (4AL, Barrero et al. 2015) and TaMKK3-A (4AL, Torada et al. 2016); there are few reports on the related QTL / genes regulating the duration of seed dormancy, To a certain extent, it hinders the cultivation of varieties with longer duration of panicle germination resistance

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