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A photoacoustic detection device and method for glucose concentration in biological fluid

A technology for glucose concentration and biological fluids, applied in the field of photoacoustic detection and analysis

Active Publication Date: 2021-06-04
NORTHEASTERN UNIV LIAONING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There have been many reports on trace gas detection devices, and some studies have focused on cancer markers in human breath. However, the miniaturized photoacoustic detection system for biological sample analysis instruments or related methods have not been reported.

Method used

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  • A photoacoustic detection device and method for glucose concentration in biological fluid
  • A photoacoustic detection device and method for glucose concentration in biological fluid
  • A photoacoustic detection device and method for glucose concentration in biological fluid

Examples

Experimental program
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Effect test

Embodiment 1

[0059] A photoacoustic detection device for glucose concentration in biological fluids, including a photoacoustic detection system and a sample reaction module. The schematic diagram of the device structure is shown in figure 1 Shown:

[0060] The photoacoustic device is composed of a laser 4, a chopper 5, a sample holder 7, a photoacoustic cell 8, a microphone 10, a preamplifier 11 and a notebook computer 12, and the laser 4 is modulated into a periodic light source through the chopper 5; The structural representation of the sample holder 7 is as image 3 As shown, the size schematic diagram of the photoacoustic cell 8 is shown as figure 2 As shown, the schematic diagram of the sample holder structure is shown in image 3 As shown, the photoacoustic pool 8 is made of polytetrafluoroethylene, and its length, width, and height are 40mm, 20mm, and 20mm respectively. The pool body is drilled. The hole A on the left side of the photoacoustic pool 8 has an aperture of 13.5mm and...

Embodiment 2

[0066] The structure of the device in this embodiment is the same as in Embodiment 1.

[0067] The method for photoacoustic detection of glucose concentration in human blood by using the above device, comprising the following steps:

[0068] Dilute the freshly obtained blood to the linear range of glucose, add 3 μL of chitosan solution with a mass concentration of 0.6% to the paper chip detection area 3, after drying at room temperature (25°C), add 2 μL of starch potassium iodide solution dropwise, and continue at room temperature Dry (25°C), then add 2 μL of glucose oxidase solution with a concentration of 2 mg / mL dropwise;

[0069] Add 20 μL of diluted human blood dropwise to the paper chip sample area 2, the diluted human blood flows to the paper chip detection area 3 through capillary action, and react at room temperature (25°C) for 30 minutes to form a purple inclusion compound; then the paper chip is detected Area 3 is transferred to the photoacoustic cell 8, the laser ...

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Abstract

A photoacoustic detection device and method for glucose concentration in biological fluids of the present invention belong to the technical field of photoacoustic detection and analysis; the device includes a photoacoustic detection device and a sample reaction module, and provides a specific structure and connection method. Using this device can Sensitive detection of glucose concentration in biological fluids; a miniaturized photoacoustic detection method is provided. The light source, chopper, and microphone used in the device can all be powered by portable power supplies, which get rid of the bulky components in the prior art and are portable It can be used for on-site detection; when the method is tested, the paper chip is used as the reaction medium, and the starch potassium iodide system is used as the medium to realize the photoacoustic detection of glucose concentration in biological fluids, and to realize the detection of other analytes that can generate hydrogen peroxide. Analysis, expanding the scope of photoacoustic detection technology for the analysis of biological samples.

Description

Technical field: [0001] The invention belongs to the technical field of photoacoustic detection and analysis, and in particular relates to a photoacoustic detection device and method for glucose concentration in biological fluid. Background technique: [0002] Photoacoustic (Photoacoustic) technology is to irradiate the sample containing the analyte with an excitation light source, so that the analyte transitions from the ground state to the excited state, and produces a thermal effect through the non-radiative transition to the ground state, and then generates sound waves, which are quantified by detecting the sound intensity Analysis techniques. Traditional absorption and emission optical detection instruments are more suitable for homogeneous samples, and have high requirements for sample morphology, matrix, and optical properties, and cannot realize optical signal detection of complex matrix samples. Photoacoustic spectroscopy can be used for the detection of analytes i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/17
CPCG01N21/1702
Inventor 于永亮张雅洁王建华
Owner NORTHEASTERN UNIV LIAONING
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