Mixed stem cell injection containing three vascular regeneration attributes and preparation method thereof

A technology of mixing stem cells and injections, which is applied in the field of stem cell injections and its preparation, can solve problems such as difficult promotion, invasiveness, and failure to fundamentally improve prognosis

Active Publication Date: 2020-08-18
高连如
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Modern medicine has failed to fundamentally improve its prognosis with drugs, intervention, surgery, and organ transplantation
Although stem cell regenerative medicine has brought new opportunities in the 21st century, the specificity, functionality, and safety of seed cell regener

Method used

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  • Mixed stem cell injection containing three vascular regeneration attributes and preparation method thereof
  • Mixed stem cell injection containing three vascular regeneration attributes and preparation method thereof
  • Mixed stem cell injection containing three vascular regeneration attributes and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0096] A mixed stem cell injection containing three kinds of angiogenesis attributes in this embodiment, the preparation steps are as follows:

[0097] S1 prepares human umbilical cord artery adventitial Flk-1+ cells as follows:

[0098] (S11) Extraction of human umbilical artery adventitia Flk-1+ cells:

[0099] Take the fresh umbilical cord from cesarean section, rinse it three times with PBS, wash off the residual blood of the umbilical cord, cut the Huatong glue along the direction of the umbilical artery, and cut the umbilical artery longitudinally along the umbilical artery lumen with ophthalmic scissors. The two layers are adventitia and intima. After tearing off the intima at the section of the cut umbilical artery with toothed ophthalmic forceps, the adventitia of the umbilical artery is torn off, and the torn umbilical artery adventitia is placed in a sterile dish Cut into as small crumbs as possible. Put the stripped umbilical artery adventitia fragments into a st...

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Abstract

The invention relates to a mixed stem cell injection containing three vascular regeneration attributes. The mixed stem cell injection comprises Wharton jelly mesenchymal stem cells (WJMSCs), human umbilical artery adventitia periodontal cells (APCs) and human umbilical artery adventitia Flk-1 + cells. Umbilical cords abandoned by newborns are used as a material source; the method comprises the following steps: extracting Flk-1 + cells and umbilical artery peripheral cells APC from umbilical artery adventitia; the two cells provide two direct and specific precursor cells for vascular regeneration, and through the extracellular matrix components of the new blood vessel supplemented, updated and formed by the WJMSCs, a regeneration microenvironment for differentiation of the vascular precursor cells into the blood vessel under regulation and control of a vascular regeneration signal system is further formed. The three stem cells are mixed in proportion for an in-vitro culture experiment,and the result proves that the vascular net is successfully formed after in-vitro culture is performed for 13 days. The injection provided by the invention can be prepared into an injection for vascular regeneration, and is used for treating ischemic heart disease, ischemic stroke, ischemic peripheral vascular disease and other diseases.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to an injection containing stem cells with various vascular regeneration properties and a preparation method thereof. Background technique [0002] Ischemic diseases caused by vascular lesions, such as coronary heart disease, cerebral infarction, ischemic necrosis of limbs, and even eye ischemic blindness, etc., have been the top causes of death and disability in the world so far. Modern medicine has failed to fundamentally improve its prognosis with drugs, intervention, surgery, and organ transplantation. Although stem cell regenerative medicine has brought new opportunities in the 21st century, the specificity, functionality, and safety of seed cell regeneration of blood vessels have greatly restricted the realization of the concept of stem cell regeneration of blood vessels. [0003] At present, although embryonic stem cells (ESC) and induced pluripotent stem cells ...

Claims

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Application Information

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IPC IPC(8): A61K35/51A61K9/08A61K47/42A61P9/10A61K35/28
CPCA61K9/0019A61K9/08A61K35/28A61K35/51A61K47/42A61P9/10A61K2300/00
Inventor 高连如张宁坤
Owner 高连如
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