Stem cell drug fused with PNV targeted ischemic myocardial blood vessels and preparation method

A stem cell and cardiovascular technology, applied in the field of bioengineering, can solve problems such as DNA mutation, easy immune response, and limited environmental conditions

Active Publication Date: 2020-12-18
高连如
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the presence of antibody FC, it is easy to be phagocytized by immune cells in the body, so the effect is poor and it is easy to stimulate the immune response
In addition, there are also studies on the method of using an external magnetic field to guide the homing of stem cells, which is limited by environmental conditions, etc.
The recent genetic modification method expresses the single-chain antibody linkage method, and there is still a risk of DNA mutation due to gene introduction into DNA
Therefore, so far, there is no safe and reliable method to solve the problem of targeted homing of exogenously infused stem cells to ischemic myocardial vessels

Method used

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  • Stem cell drug fused with PNV targeted ischemic myocardial blood vessels and preparation method
  • Stem cell drug fused with PNV targeted ischemic myocardial blood vessels and preparation method
  • Stem cell drug fused with PNV targeted ischemic myocardial blood vessels and preparation method

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preparation example Construction

[0062] The platelet membrane nanovesicles were prepared by using a water-bath sonicator to process for 5 minutes under ultrasonic conditions with a frequency of 42 kHz and a power of 100 W. The presence of PNV was verified by morphological examination using dynamic light scattering (DLS) and transmission electron microscopy (TEM). CD42b (GPIbα) and CD41 (IIb / IIIa) receptors were identified by Western blot, immunohistochemistry, and flow cytometry.

[0063] The more optimal technical scheme of the present invention is conceived as follows: in order to realize effective angiogenesis and myocardial repair, two kinds of cardiovascular precursor cells (APC, Flk-1+) are directly selected, and a unique microenvironment (WJMSCs) is provided for angiogenesis ), that is, the components of the extracellular matrix, thus constituting the regeneration microenvironment in which vascular precursor cells differentiate into blood vessels under the regulation of angiogenesis signaling system. ...

Embodiment 1

[0067] The stem cell drug fused with PNV targeting ischemic myocardial vessels in this embodiment is specifically an injection. The preparation process and efficacy characteristics of the injection solution fused with PNV targeting ischemic myocardial blood vessels of the present invention will be described below in conjunction with this example.

[0068] The umbilical cord blood and umbilical cord abandoned by newborns were used as material sources, and platelets were extracted from umbilical cord blood to prepare platelet membrane nanovesicles PNV. Flk-1+ cells and umbilical artery adventitial pericyte APC were extracted from the umbilical artery adventitia, and WJMSCs derived from Huatong jelly were extracted from the Huatong jelly tissue between the umbilical artery, umbilical vein and umbilical cord adventitia. The three kinds of stem cells were mixed and cultured in proportion to obtain cardiovascular precursor stem cells CVPSCs, and then PNV was fused with CVPSCs to pre...

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Abstract

The invention discloses a stem cell drug fused with PNV targeted ischemic myocardial blood vessels. The stem cell drug comprises cardiovascular precursor stem cells, and platelet membrane nano-vesicles PNV are fused on the surfaces of the cardiovascular precursor stem cells. The platelet membrane nano-vesicles PNV are prepared by the aid of the biological characteristic that human platelet membrane glycoprotein receptors naturally target to ischemic myocardium and atherosclerosis ischemic blood vessels for homing, and the cardiovascular precursor stem cells are coated with the PNV to prepare the stem cell drug. The PNV reserves the glycoprotein receptors on platelet membranes and can sense various dangerous signal molecules, does not have a mechanism for triggering activated coagulation, is a natural and safe targeted navigation substance and can carry the vascular precursor stem cells to target to the ischemic myocardial blood vessels for homing, and the damaged myocardial blood vessels are accurately repaired. Two vascular precursor cells including Flk-1+ cells and adventitial peripheral cells (APC) are further adopted and matched with WJMSCs, and the vascular precursor cells canbe directly differentiated into blood vessels with a blood perfusion function.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a stem cell medicine fused with PNV targeting ischemic myocardial vessels and a preparation method thereof. Background technique [0002] Ischemic diseases caused by vascular lesions, such as coronary heart disease, cerebral infarction, ischemic necrosis of limbs, and even eye ischemic blindness, etc., have been the top causes of death and disability in the world so far. Modern medicine has failed to fundamentally improve its prognosis with drugs, intervention, surgery, organ transplantation and other means. Stem cell regenerative medicine in the 21st century has brought new opportunities. But unfortunately, because the transplanted cells can rarely reach the diseased organ tissue, the retention time is short, and the 24-hour retention is less than 5-10%, which creates a great obstacle to the realization of stem cell repair of myocardial regeneration blood vessels. [00...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/69A61K35/51A61K47/42A61K9/08C12N5/0775C12N5/071C12N5/078A61P9/10A61P9/00
CPCA61K9/0019A61K9/08A61K35/51A61K47/42A61K47/6901A61P9/00A61P9/10C12N5/0644C12N5/0668C12N5/069C12N2501/02C12N2501/734C12N2509/00
Inventor 高连如张宁坤
Owner 高连如
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