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Pyrite nanozyme, antitumor drug and application

A technology of nanozyme and pyrite, which is applied in the field of nanomaterials and tumor treatment, can solve few problems and achieve excellent safety effects

Active Publication Date: 2022-02-18
INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, such combined apoptosis-ferroptosis strategies are currently rare

Method used

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  • Pyrite nanozyme, antitumor drug and application
  • Pyrite nanozyme, antitumor drug and application
  • Pyrite nanozyme, antitumor drug and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Prepared as follows:

[0039] S1 Disperse 0.7g PVP in 30mL ethylene glycol solution, add 0.5 g FeCl under constant stirring 3 .6H 2 O, get A;

[0040] S2 Stir A vigorously, and add 3.6 g NaAc while stirring to obtain B;

[0041] S3 Add 0.4 g of S powder to B, and ultrasonically react for 1 hour to obtain C;

[0042] S4 Transfer C to a 40mL polytetrafluoroethylene-lined stainless steel autoclave, seal and keep it at 473K for a high temperature reaction for 12 hours, and cool to room temperature after the reaction to obtain D;

[0043] S5 Centrifuge D at 10,000 rpm for 10 minutes to collect a black precipitate, wash the collected black precipitate with chloroform to remove the residual S, then wash with absolute alcohol and ultrapure water to remove impurities, centrifuge and store in a vacuum freeze dryer Dry overnight.

Embodiment 2

[0045] Prepared as follows:

[0046] S1 Disperse 0.6g PVP in 30mL ethylene glycol solution, add 0.5 g FeCl under continuous stirring 3 .6H 2 O, get A;

[0047] S2 Stir A vigorously, and add 3.6 g NaAc while stirring to obtain B;

[0048] S3 Add 0.3 g of S powder to B, ultrasonically react for 1 hour, and obtain C;

[0049] S4 Transfer C to a 40mL polytetrafluoroethylene-lined stainless steel autoclave, seal and keep it at 473K for a high temperature reaction for 12h, and cool to room temperature after the reaction to obtain D;

[0050] S5 Centrifuge D at 10,000 rpm for 10 minutes to collect a black precipitate, wash the collected black precipitate with chloroform to remove the residual S, then wash with absolute alcohol and ultrapure water to remove impurities, centrifuge and store in a vacuum lyophilizer Dry overnight.

Embodiment 3

[0052] Prepared as follows:

[0053] S1 Disperse 0.8g of PVP in 30mL of ethylene glycol solution, add 0.5g of FeCl under continuous stirring 3 .6H 2 O, get A;

[0054] S2 Stir A vigorously, and add 3.6 g NaAc while stirring to obtain B;

[0055] S3 Add 0.6g of S powder to B, ultrasonically react for 1 hour, and obtain C;

[0056]S4 Transfer C to a 40mL polytetrafluoroethylene-lined stainless steel autoclave, seal and keep it at 473K for a high temperature reaction for 12 hours, and cool to room temperature after the reaction to obtain D;

[0057] S5 Centrifuge D at 10,000 rpm for 10 minutes to collect a black precipitate, wash the collected black precipitate with chloroform to remove the residual S, then wash with absolute alcohol and ultrapure water to remove impurities, centrifuge and store in a vacuum freeze dryer Dry overnight.

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Abstract

The invention provides a pyrite nanozyme, an antineoplastic drug and its application. The pyrite nanozyme includes Fe 2+ and S 2 2‑ , the atomic ratio of Fe and S is 1:2, presenting FeS 2 crystal form. The pyrite nanozyme has both peroxidase activity and glutathione oxidase activity. The POD and GSH‑OXD activities of pyrite nanozyme can constitute a cascade reaction, causing tumor cells to produce OH and deplete intracellular GSH, leading to apoptosis and ferroptosis of tumor cells; the killing effect is specific to tumor cells, and for normal The cell killing effect is very weak; it is degradable and ensures excellent safety; it can exert POD and GSH‑OXD activities in tumor cells in vivo by causing tissue apoptosis and ferroptosis, and it is resistant to apoptosis in KRAS mutations Excellent therapeutic effect was achieved in CT26 tumors.

Description

technical field [0001] The invention relates to the technical field of nanomaterials and tumor treatment, in particular to pyrite nanozymes, antitumor drugs and applications. Background technique [0002] Since Fe was first reported in 2007 3 o 4 Nanoparticles have peroxidase-like activity, and nanozymes, a class of nanomaterials with inherent enzymatic properties, have formed an emerging field. As a new generation of mimic enzymes, nanozymes are economical, stable, and easy to produce in large quantities. More importantly, nanozymes combine the catalytic activity of enzymes with the unique physical and chemical properties of nanomaterials, making it a multifunctional molecule. Different nanozymes have been widely used as substitutes for natural enzymes in molecular detection, immunoassay, antibacterial, and environmental governance. In particular, the ability of nanozymes to regulate reactive oxygen species (Reactive oxygen species, ROS) can be used in tumor therapy, suc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K33/26A61K47/54A61K47/68A61P35/00B82Y5/00C01G49/12
CPCA61K33/26B82Y5/00A61P35/00A61K47/68A61K47/546A61K47/545C01G49/12C01P2002/72C01P2002/85C01P2004/03C01P2004/32C01P2004/62
Inventor 阎锡蕴高利增范克龙孟祥芹
Owner INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
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