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Extraction method for optimizing alpha-glucosidase inhibitor in pomegranate flower by response surface method

A technology of glucosidase and response surface method, which is applied in the field of natural product extraction, can solve the problems that the yield of α-glucosidase inhibitors does not reach the optimal level, loss of ethanol solvent, high price of ethanol, etc., and achieves good inhibitory effect , large component content, and the effect of shortening the extraction time

Active Publication Date: 2021-08-27
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this patent only investigated the influence of different solvent ratios and extraction methods on α-glucosidase inhibitors, and did not investigate the influence of solid-liquid ratio, temperature and time, resulting in the yield of α-glucosidase inhibitors not reaching Optimal

Method used

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  • Extraction method for optimizing alpha-glucosidase inhibitor in pomegranate flower by response surface method
  • Extraction method for optimizing alpha-glucosidase inhibitor in pomegranate flower by response surface method
  • Extraction method for optimizing alpha-glucosidase inhibitor in pomegranate flower by response surface method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1 (response surface method)

[0027] a. the picked pomegranate flowers are dried, pulverized, and passed through a 100-200 mesh sieve to obtain pomegranate flower powder;

[0028] b. Utilize Design Expert 10 software to carry out Box-Behnken response surface optimization design with the pomegranate flower powder obtained in step a, use the IC of pomegranate flower extract anti-α-glucosidase activity 50 The value is the response value, using three factors and three levels of response surface analysis to optimize the ultrasonic extraction of α-glucosidase inhibitors in pomegranate flowers, the liquid-to-material ratio is 1:20g / mL, the solvent ratio is 45% ethanol water, and the ultrasonic time 3h, the extraction solution was obtained under the condition of an ultrasonic temperature of 45°C;

[0029] c. Centrifuge the extract obtained in b at 7000rpm for 10min, get the supernatant concentrated and dried and weighed to obtain the α-glucosidase inhibitor in pomeg...

Embodiment 2

[0069] A response surface method of the present invention optimizes the hypoglycemic experiment of α-glucosidase inhibitors in pomegranate flowers:

[0070] α-glucosidase activity assay:

[0071] Mix 50 μL sample solution with appropriate concentration with 50 μL 0.2 U / mL α-glucosidase, react at room temperature for 6 min, then add 50 μL 5.0 mM p-nitrophenyl-D-glucopyranoside (p-nitrophenyl-D-glucopyranoside ) (0.1MPB, pH 6.8), react at 37°C for 10min, add 100μL of 0.2M sodium carbonate to the reaction system to terminate the reaction, measure the absorbance at 405nm, acarbose is used as a positive control, and the results are measured by IC 50 The value indicates that the enzyme inhibition calculation formula is:

[0072]

[0073] Sample group As: reaction system with sample and enzyme; control group Ac: reaction system without sample; blank group Aj: reaction system without enzyme and sample;

[0074] Carry out in vitro α-glucosidase inhibition experiment on the commerc...

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Abstract

The invention discloses an extraction method for optimizing an alpha-glucosidase inhibitor in pomegranate flower by a response surface method. The method comprises the steps that the pomegranate flowers serve as raw materials, and firstly, the influence of a solvent proportion, a material-liquid ratio, ultrasonic time and ultrasonic temperature on the alpha-glucosidase inhibitor in the pomegranate flowers is investigated through a single-factor experiment; response surface optimization treatment is performed according to the single factor experiment result to obtain a pomegranate flower extracting solution, and the extracting solution is concentrated and dried to obtain the alpha-glucosidase inhibitor. The process is simple, safe and reliable, and a theoretical basis and an experimental basis are provided for development and utilization of pomegranate flowers for treating diabetes mellitus.

Description

technical field [0001] The invention belongs to the technical field of natural product extraction, and relates to a response surface method to optimize the extraction method of alpha-glucosidase inhibitors in pomegranate flowers. Background technique [0002] α-glucosidase (α-glucosidase), also known as glucosyltransferase, is a class of enzymes that can catalyze the hydrolysis of α-glucosidyl from the non-reducing end of substrates containing α-glucosidic bonds, distributed in the small intestinal epithelial chorion On the brush edge, the non-reducing terminal α-1,4 glycosidic bonds of α-glucosides, oligosaccharides and glucans can be cleaved by hydrolysis to release glucose, resulting in increased blood sugar levels, which can cause diabetes or diabetes complication. Therefore, oral administration of α-glucosidase inhibitors can slow down the production and absorption of glucose, reduce postprandial blood sugar peaks, and maintain blood sugar at a dynamic equilibrium leve...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61P3/10A23L33/105A61K133/00
CPCA61K36/185A61P3/10A23L33/105A61K2236/333A23V2002/00A23V2200/328
Inventor 袁涛贾丽阿吉艾克拜尔·艾萨热依木古丽·阿布都拉
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI