Method for rapidly restoring the denitrification performance of a short-cut nitrification-anaerobic ammonium oxidation pilot system
By short-term addition of C6-HSL signal molecules in the anaerobic ammonia oxidation system, the induction ability of microbial groups is enhanced, and the problem of unstable nitrogen removal performance of the system during water quality fluctuations or shutdowns is solved, and the effect of rapid recovery of nitrogen removal performance is achieved. .
Patent Information
- Application Number
- CN202411194830.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-29
- Publication Date
- 2025-02-11
- Estimated Expiration
- 2044-08-29
AI Technical Summary
During the fluctuation or shutdown of the water quality of the sewage treatment plant, the anaerobic ammonia oxidation system can easily lead to unstable or deterioration of the nitrogen removal performance, and lack technical means for rapid recovery.
By short-term exogenous addition of C6-HSL signal molecules in the short-term nitrification-anaerobic ammonia oxidation pilot system, the population sensing ability and metabolic activity between functional bacteria can be enhanced, thereby quickly restoring nitrogen removal performance.
The rapid nitrogen-destruction performance recovery of the anaerobic ammonia oxidation system was achieved, and the activities of ammonia oxidation bacteria and anaerobic ammonia oxidation bacteria were increased by 20.02% and 93.88% respectively, with simple operation, short time-consuming and significant increase in nitrogen-destruction performance.
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Figure CN118724281B_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the technical field of sewage treatment, and in particular to a method for rapidly recovering the denitrification performance of a short-range nitrification-anaerobic ammonium oxidation pilot system. Background Art
[0002] Anaerobic ammonium oxidation is a new environmentally friendly biological denitrification process. Compared with the traditional nitrification and denitrification process, the integrated short-range nitrification-anaerobic ammonium oxidation process has the advantages of saving 60% of aeration volume, not requiring an organic carbon source, and reducing sludge production. However, the generation cycle of anaerobic ammonium oxidizing bacteria is long, and its metabolic activity is easily affected by environmental factors such as substrate concentration, temperature, and dissolved oxygen. In actual application, due to fluctuations in water quality in sewage treatment plants or shutdowns for weeks or months for annual inspections and maintenance, the anaerobic ammonium oxidation system has to face the problem of unstable or deteriorated denitrification performance. Therefore, how to quickly restore an anaerobic ammonium oxidation system with deteriorated denitrification performance is a technical problem that urgently needs to be solved in this field.
[0003] Quorum sensing refers to the regulatory mechanism by which microorganisms secrete and sense signal molecules to mediate gene expression and physiological behavior among groups. In an integrated short-range nitrification-anammox system, the cooperation and competition between ammonia oxidizing bacteria, nitrite oxidizing bacteria, anammox bacteria and denitrifying bacteria are usually regulated by quorum sensing mediated by signal molecules (such as homoserine lactone AHLs). At present, a variety of AHLs-type signal molecules have been detected in short-range nitrification-anammox systems, and these signal molecules play an important role in regulating the expression of functional genes. Therefore, by enhancing the quorum sensing ability of microorganisms in the anammox system, the denitrification functional bacteria can better coordinate group behavior and improve metabolic activity, thereby quickly restoring the denitrification performance of the anammox system. At present, most studies on the effects of exogenous addition of AHLs-type signal molecules on the anammox system use artificial water distribution and smaller reactors for experiments. However, compared with actual sewage, artificial water distribution has the characteristics of single composition and stable water quality, which cannot provide strong technical support for the optimized operation of anammox systems that treat actual complex water quality.
[0004] Due to the above defects in the prior art, it is difficult to provide a theoretical basis and guidance for the complex water quality conditions in actual projects. It can be seen that the prior art needs to be further improved. Summary of the invention
[0005] The object of the present invention is to provide a method for quickly recovering the denitrification performance of a short-range nitrification-anaerobic ammonium oxidation pilot system, which does not require changing the original operating mode. By short-term exogenous addition of C6-HSL signal molecules, the quorum sensing ability and metabolic activity between functional bacteria are enhanced, thereby accelerating the recovery of the denitrification performance of the side-stream short-range nitrification-anaerobic ammonium oxidation pilot system.
[0006] In order to achieve the above-mentioned purpose, the present invention adopts the following technical scheme: a method for quickly restoring the denitrification performance of a short-range nitrification-anaerobic ammonium oxidation pilot system, comprising the following steps in sequence: step 1, installing a reserve bottle and a metering pump in an existing short-range nitrification-anaerobic ammonium oxidation pilot system, the short-range nitrification-anaerobic ammonium oxidation pilot system comprising a short-range nitrification-anaerobic ammonium oxidation pilot reactor, an agitator, a heater, a water tank and a PLC control system; the metering pump is installed on a pipe connecting the reserve bottle and the water inlet of the short-range nitrification-anaerobic ammonium oxidation pilot reactor.
[0007] The short-range nitrification-anaerobic ammonium oxidation pilot reactor comprises a reactor body and a perforated aeration pipe located in the reactor body. A water inlet, a drain outlet, an overflow outlet and a sampling port are respectively arranged on the side wall of the reactor body. Suspended fillers are added into the reactor body.
[0008] The reserve bottle is used to provide C6-HSL signal molecule reserve solution to the short-range nitrification-anaerobic ammonium oxidation pilot reactor; the water tank is used to provide sludge digestion liquid to the short-range nitrification-anaerobic ammonium oxidation pilot reactor; the PLC control system is used to realize automatic control of the short-range nitrification-anaerobic ammonium oxidation pilot system.
[0009] Step 2. The water quality characteristic parameters of the sludge digestion liquid are: ammonia nitrogen concentration is 273~466 mg / L, chemical oxygen demand COD concentration is 149~382 mg / L, and C / N ratio is 0.52~1.50.
[0010] Step 3, the short-range nitrification-anaerobic ammonium oxidation pilot reactor adopts a sequencing batch reaction, and the single cycle operation time is 1440~2880 min. The operation mode is as follows: the sludge digestion liquid and the C6-HSL signal molecule reserve solution are added to the reactor body through the PLC control system; after the sludge digestion liquid and the C6-HSL signal molecule reserve solution are completed, the agitator is turned on for pre-anoxic stirring; then intermittent aeration is performed, and the intermittent aeration operation mode includes aerobic aeration and anoxic stirring, and the time ratio of aerobic aeration and anoxic stirring is 30:(30~60) min, so that the dissolved oxygen concentration in the reactor body is controlled at 0.3~0.8 mg / L; after the reaction is completed, precipitation is carried out, and the water is discharged through the drain port, and the drainage ratio is 50%; after drainage, it is idle for 25 min, and the hydraulic retention time is 48~96 h.
[0011] Step 4: Recycle step 3. After 12 cycles of adding C6-HSL signal molecules, the activity of ammonia oxidizing bacteria increased by 20.02%; the activity of anaerobic ammonia oxidizing bacteria increased by 93.88%; at this time, stop adding C6-HSL signal molecules.
[0012] In the above-mentioned method for rapidly restoring the denitrification performance of the short-range nitrification-anaerobic ammonium oxidation pilot system, in step one, the filling volume ratio of the suspended filler is 40% to 50% of the volume of the reactor body; the perforated aeration pipe is connected to the aeration pump through a gas flow meter, the aeration pump is connected to the PLC control system, and the automatic operation of the aeration pump is controlled by the PLC control system.
[0013] The above-mentioned method for quickly restoring the denitrification performance of the short-range nitrification-anaerobic ammonium oxidation pilot system, the agitator is used to fluidize the filler in the short-range nitrification-anaerobic ammonium oxidation pilot reactor, the agitator includes a motor and a rotating shaft, a stirring paddle is provided on the rotating shaft, and the motor drives the stirring paddle on the rotating shaft to rotate to achieve filler fluidization; the heater is used to provide the required temperature to the short-range nitrification-anaerobic ammonium oxidation pilot reactor; the heater includes at least one heating belt wrapped around the outer wall of the reactor body, the temperature of the reactor body is adjusted by the heating belt, the operating temperature of the reactor body is 30±2°C, and it is operated away from light.
[0014] The above-mentioned method for quickly restoring the denitrification performance of the short-range nitrification-anaerobic ammonium oxidation pilot system, the short-range nitrification-anaerobic ammonium oxidation pilot system also includes a dissolved oxygen probe, a pH probe and a water quality analyzer, the dissolved oxygen probe and the pH probe are both connected to the water quality analyzer, the dissolved oxygen concentration in the reactor body is detected by the dissolved oxygen probe, and the pH in the reactor body is detected by the pH probe; a submersible sewage pump is provided on the pipeline connecting the water tank and the water inlet of the short-range nitrification-anaerobic ammonium oxidation pilot reactor, and a metering pump is provided on the pipeline connecting the reserve bottle and the water inlet of the short-range nitrification-anaerobic ammonium oxidation pilot reactor; the submersible sewage pump and the metering pump are both connected to the PLC control system.
[0015] The above method for rapidly restoring the denitrification performance of the short-range nitrification-anaerobic ammonium oxidation pilot system is provided with an electric drain valve near the drain outlet, and the electric drain valve is connected to the PLC control system.
[0016] In the above-mentioned method for quickly restoring the denitrification performance of the short-range nitrification-anaerobic ammonium oxidation pilot system, in step three, the speed of the agitator during pre-anoxic stirring is 70~100 r / min, and the pre-anoxic stirring time is 100~140 min; the intermittent aeration time is 1260~2700 min, and the aeration volume is 0.3~0.5 L / min; the sedimentation time after the reaction is completed is 2 min.
[0017] In the above-mentioned method for rapidly restoring the denitrification performance of the short-range nitrification-anaerobic ammonium oxidation pilot system, in step three, the concentration of the C6-HSL signal molecule in the reactor body is ensured to be 10-100 nmol / L each time it is added.
[0018] Compared with the prior art, the present invention brings the following beneficial technical effects: (1) The present invention takes the short-term nitrification-anaerobic ammonium oxidation pilot system for treating actual sludge digestion liquid as the research object. Under the premise of not changing the original operation mode, the present invention realizes the rapid recovery of anaerobic ammonium oxidation activity in the side-stream short-term nitrification-anaerobic ammonium oxidation pilot system by only adding a small amount of C6-HSL signal molecules for a short period of time. The operation is simple, the time consumption is short, the denitrification performance is significantly improved, and it is obviously advanced.
[0019] (2) The present invention is the first to apply the C6-HSL signal molecule to quickly restore the denitrification performance of an integrated short-range nitrification-anaerobic ammonium oxidation pilot system using actual sludge digestion liquid as the influent matrix. This is different from the small-volume reactor test mode using artificial water distribution. The method of the present invention has a wider applicability and is more meaningful for practical engineering guidance.
[0020] (3) The C6-HSL signal molecule of the present invention is added at a low concentration, and an operation strategy of low dissolved oxygen and intermittent aeration is adopted during operation. No external organic carbon source is required, the operation energy consumption is low, and the operation cost is saved.
[0021] (4) The present invention is applicable to both the anaerobic ammonium oxidation combined process and the pure anaerobic ammonium oxidation process, and has a wide range of applications.
[0022] In summary, the present invention uses actual sludge digestion liquid as the influent matrix, and through exogenous addition of C6-HSL signal molecules, regulates the quorum sensing among system microorganisms, thereby increasing the activities of ammonia oxidizing bacteria and anaerobic ammonia oxidizing bacteria by 20.02% and 93.88% respectively in a short period of time, thereby achieving rapid recovery of the denitrification performance of the side-stream short-range nitrification-anaerobic ammonia oxidation pilot system. BRIEF DESCRIPTION OF THE DRAWINGS
[0023] Figure 1 It is a schematic diagram of the structure of the short-range nitrification-anaerobic ammonium oxidation pilot system of the present invention.
[0024] In the figure: 1. water tank, 2. submersible sewage pump, 3. reserve bottle, 4. metering pump, 5. water inlet, 6. short-range nitrification-anaerobic ammonium oxidation pilot reactor, 7. aeration pump, 8. gas flow meter, 9. perforated aeration pipe, 10. heating belt, 11. agitator, 12. drain outlet, 13. electric drain valve, 14. sampling port, 15. overflow port, 16. water quality analyzer, 17. dissolved oxygen probe, 18. pH probe, 19. PLC control system. DETAILED DESCRIPTION
[0025] The technical solutions in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application.
[0026] In the description of the present application, the terms "includes" and "has" and any variations thereof are intended to cover a non-exclusive inclusion.
[0027] The working principle of the PLC control system 19 described in the present invention can be realized by those skilled in the art by referring to the existing technology.
[0028] like Figure 1 As shown, the short-range nitrification-anaerobic ammonium oxidation pilot system of the present invention is already available in the prior art. The present invention improves the existing system by adding a reserve bottle 3 and a metering pump 4 without changing its original operating mode. The metering pump 4 is used to add C6-HSL signal molecules to the short-range nitrification-anaerobic ammonium oxidation pilot reactor 6 to accelerate the recovery of the denitrification performance of the side stream short-range nitrification-anaerobic ammonium oxidation pilot system.
[0029] The existing short-cut nitrification-anaerobic ammonium oxidation pilot system described in the present invention includes a short-cut nitrification-anaerobic ammonium oxidation pilot reactor 6, an agitator 11, a heater, a water tank 1, a PLC control system 19, and a water quality analyzer 16.
[0030] The short-range nitrification-anaerobic ammonium oxidation pilot reactor 6 includes a reactor body and a perforated aeration pipe 9 located in the reactor body. A water inlet 5, a drain port 12, an overflow port 15 and a sampling port 14 are respectively arranged on the side walls of the reactor body, and a suspended filler is added into the reactor body; the water inlet 5 is connected to the reserve bottle 3 through a pipeline, and the reserve bottle 3 contains a C6-HSL signal molecule reserve solution. The C6-HSL signal molecule reserve solution is provided to the short-range nitrification-anaerobic ammonium oxidation pilot reactor 6 through the reserve bottle 3. A metering pump 4 is arranged on the pipeline connecting the reserve bottle 3 and the water inlet 5. The metering pump 4 is connected to a PLC control system 19. The metering pump 4 is controlled by the PLC control system 19 to control the dosage of the C6-HSL signal molecule entering the short-range nitrification-anaerobic ammonium oxidation pilot reactor 6.
[0031] The sludge digestion liquid is provided to the short-cut nitrification-anaerobic ammonium oxidation pilot reactor 6 through the water tank 1. A submersible sewage pump 2 is provided on the pipeline connecting the water tank 1 and the water inlet 5. The submersible sewage pump 2 is connected to the PLC control system 19, and the submersible sewage pump 2 is controlled by the PLC control system 19. An electric drain valve 13 is provided on the pipeline connected to the drain port 12. The electric drain valve 13 is connected to the PLC control system 19, and the electric drain valve 13 is automatically controlled by the PLC control system 19. The water quality analyzer 16 is connected to the dissolved oxygen probe 17 and the pH probe 18, and the dissolved oxygen probe 17 and the pH probe 18 are both placed below the liquid level of the reactor body of the short-cut nitrification-anaerobic ammonium oxidation pilot reactor 6.
[0032] The agitator 11 includes a motor and a rotating shaft, on which a stirring paddle is arranged, and the motor drives the stirring paddle on the rotating shaft to rotate to realize the fluidization of the filler; the heater is used to provide the required temperature to the short-range nitrification-anaerobic ammonia oxidation pilot reactor 6; the heater includes at least one heating belt 10 wound around the outer wall of the reactor body, and the temperature of the reactor body is adjusted by the heating belt 10. The operating temperature of the reactor body is 30±2°C, and it is operated away from light.
[0033] The perforated aeration pipe 9 is located at the bottom of the reactor body and is connected to the aeration pump 7 located outside the reactor body. A gas flow meter 8 is provided on the pipeline connecting the aeration pump 7 and the perforated aeration pipe 9. The aeration pump 7 is connected to a PLC control system 19, and its aeration is controlled by the PLC control system 19.
[0034] The following is a detailed description of the method for using the improved short-cut nitrification-anaerobic ammonium oxidation pilot system of the present invention.
[0035] The sludge digestion liquid and C6-HSL signal molecule reserve solution in the water tank 1 and the reserve bottle 3 are pumped into the short-range nitrification-anaerobic ammonium oxidation pilot reactor 6 through the submersible sewage pump 2 and the metering pump 4 respectively. At the same time, the stirrer 11 is turned on to ensure the fluidization of the filler. The aeration intensity is adjusted by the aeration pump 7 and the gas flow meter 8. The temperature of the short-range nitrification-anaerobic ammonium oxidation pilot reactor 6 is adjusted by the heating belt 10. The dissolved oxygen concentration, pH and temperature in the short-range nitrification-anaerobic ammonium oxidation pilot reactor 6 are detected by the dissolved oxygen probe 17 and the pH probe 18. The drainage is controlled by the drain port 12 and the electric drain valve 13. The automatic operation of the submersible sewage pump 2, the metering pump 4, the aeration pump 7, the heating belt 10, the stirrer 11 and the electric drain valve 13 is controlled by the PLC control system 19.
[0036] In combination with the above-mentioned improved short-cut nitrification-anaerobic ammonium oxidation pilot system and its use method, the method of the present invention for quickly restoring the denitrification performance of the short-cut nitrification-anaerobic ammonium oxidation pilot system is further described, which specifically includes the following steps: Step 1, installing the above-mentioned improved short-cut nitrification-anaerobic ammonium oxidation pilot system.
[0037] Step 2: The water quality characteristic parameters of the sludge digestion liquid in the water tank 1 are: ammonia nitrogen concentration is 273~466 mg / L, chemical oxygen demand COD concentration is 149~382 mg / L, and C / N ratio is 0.52~1.50.
[0038] Step 3, the short-range nitrification-anaerobic ammonia oxidation pilot reactor 6 adopts a sequencing batch reaction, and the single cycle operation time is 1440~2880 min. The operation mode is as follows: the sludge digestion liquid and the C6-HSL signal molecule reserve solution are added to the reactor body through the PLC control system 19; when the sludge digestion liquid and the C6-HSL signal molecule reserve solution are completed, the agitator 11 is turned on for pre-anoxic stirring; then intermittent aeration is performed, and the intermittent aeration operation mode includes aerobic aeration and anoxic stirring, and the time ratio of aerobic aeration and anoxic stirring is 30: (30~60) min, so that the dissolved oxygen concentration in the reactor body is controlled at 0.3~0.8 mg / L; after the reaction is completed, precipitation is carried out, and drainage is carried out through the drain port 12, and the drainage ratio is 50%; after drainage, it is idle for 25 min, and the hydraulic retention time is 48~96 h.
[0039] Step 4: Recycle step 3. After 12 cycles of adding C6-HSL signal molecules, the activity of ammonia oxidizing bacteria increased by 20.02%; the activity of anaerobic ammonia oxidizing bacteria increased by 93.88%; at this time, stop adding C6-HSL signal molecules.
[0040] The present invention will be further described below in conjunction with specific embodiments.
[0041] Example 1: In the water tank 1 of this example, sludge digestion liquid from a sewage treatment plant in Qingdao is used as raw water, and its specific water quality is shown in Table 1.
[0042] Table 1 Water quality characteristics of sludge digestion liquid
[0043]
[0044] In this embodiment, the short-cut nitrification-anaerobic ammonium oxidation pilot reactor 6 adopts a sequencing batch reactor with an effective volume of 800 L. The material of the reactor body is organic glass, and the filling volume ratio of the suspended filler in the reactor body is 40%~50%. The operating temperature of the short-cut nitrification-anaerobic ammonium oxidation pilot reactor 6 is controlled by the heating belt 10 to be 30±2°C, and it is operated in the dark.
[0045] The following is a detailed description of the method for rapidly restoring the denitrification performance of the short-range nitrification-anaerobic ammonium oxidation pilot system.
[0046] First, the sludge digestion liquid in the water tank 1 and the C6-HSL signal molecule reserve solution in the reserve bottle 3 are added to the reactor body through the submersible sewage pump 2 and the metering pump 4. Preferably, the concentration of the C6-HSL signal molecule in the reactor body reaches 20 nmol / L, and a total of 12 cycles are added.
[0047] Secondly, the batch operation mode is adopted. After the sludge digestion liquid and C6-HSL signal molecule reserve liquid are fed, the agitator 11 is turned on for 120 min of pre-anoxic stirring, and then the aeration pump 7 is turned on for intermittent aeration for 1260-2700 min. The operation mode of intermittent aeration is aerobic aeration: anoxic stirring = 30: (30-60) min. In the aerobic aeration stage, the flow rate is controlled at 0.3-0.5 L / min by the gas flow meter 8, so that the dissolved oxygen concentration in the reactor body is controlled at 0.3-0.8 mg / L. After the reaction is completed and 2 min of sedimentation is allowed, the electric drain valve 13 is started to drain for 23 min, the drainage ratio is 50%, and it is idle for 25 min after drainage. The hydraulic retention time is 48 h-96 h.
[0048] Finally, after 12 cycles of continuous exogenous addition of C6-HSL signal molecules, the activity of ammonia oxidizing bacteria increased from 15.59 mgN / L·h to 18.71 mg N / L·h, an increase of 20.02%; the activity of anaerobic ammonia oxidizing bacteria increased from 11.21 mg N / L·h to 21.74 mg N / L·h, an increase of 93.88%, successfully restoring the denitrification performance of the side-stream short-range nitrification-anaerobic ammonia oxidation moving bed biofilm pilot system, and the addition of C6-HSL signal molecules was stopped; 110 days after the addition of C6-HSL signal molecules was stopped, the side-stream short-range nitrification-anaerobic ammonia oxidation moving bed biofilm pilot system still maintained good denitrification performance, and its total nitrogen removal load reached 0.24 Kg N / m 3 ·d.
[0049] Parts not described in the present invention can be implemented by referring to the existing technology.
[0050] It should be noted that those skilled in the art should recognize that the above implementation modes are only used to illustrate the present application and are not used as limitations on the present application. As long as they are within the essential spirit of the present application, appropriate changes and modifications made to the above embodiments are within the scope of protection required by the present application.
Claims
1. A method for rapidly restoring the denitrification performance of a short-range nitrification-anaerobic ammonium oxidation pilot system, characterized in that: The following steps are included in sequence: Step 1: Install a reserve bottle and a metering pump in an existing short-range nitrification-anaerobic ammonium oxidation pilot system, wherein the short-range nitrification-anaerobic ammonium oxidation pilot system comprises a short-range nitrification-anaerobic ammonium oxidation pilot reactor, an agitator, a heater, a water tank and a PLC control system; the metering pump is installed on a pipe connecting the reserve bottle and the water inlet of the short-range nitrification-anaerobic ammonium oxidation pilot reactor; The short-cut nitrification-anaerobic ammonium oxidation pilot reactor comprises a reactor body and a perforated aeration pipe located in the reactor body, a water inlet, a drain outlet, an overflow outlet and a sampling port are respectively arranged on the side wall of the reactor body, and a suspended filler is added into the reactor body; The reserve bottle is used to provide C6-HSL signal molecule reserve solution to the short-range nitrification-anaerobic ammonium oxidation pilot reactor; the water tank is used to provide sludge digestion liquid to the short-range nitrification-anaerobic ammonium oxidation pilot reactor; the PLC control system is used to realize automatic control of the short-range nitrification-anaerobic ammonium oxidation pilot system; Step 2, the water quality characteristic parameters of the sludge digestion liquid are: ammonia nitrogen concentration of 273-466 mg / L, chemical oxygen demand COD concentration of 149-382 mg / L, C / N ratio of 0.52-1.50; Step 3, the short-range nitrification-anaerobic ammonium oxidation pilot reactor adopts a sequencing batch reaction, and the single cycle operation time is 1440~2880 min. The operation mode is as follows: the sludge digestion liquid and the C6-HSL signal molecule reserve solution are added to the reactor body through the PLC control system; when the sludge digestion liquid and the C6-HSL signal molecule reserve solution are completed, the agitator is turned on for pre-anoxic stirring; then intermittent aeration is performed, and the intermittent aeration operation mode includes aerobic aeration and anoxic stirring, and the time ratio of aerobic aeration and anoxic stirring is 30min: (30~60) min, so that the dissolved oxygen concentration in the reactor body is controlled at 0.3~0.8 mg / L; after the reaction is completed, precipitation is performed, and drainage is carried out through the drain port, and the drainage ratio is 50%; after drainage, it is idle for 25 min, and the hydraulic retention time is 48~96 h; Step 4: Recycle step 3. After 12 cycles of C6-HSL signal molecule addition, the activity of ammonia oxidizing bacteria increased by 20.02%; the activity of anaerobic ammonia oxidizing bacteria increased by 93.88%; at this time, stop adding C6-HSL signal molecule; In step three, each time the C6-HSL signal molecule is added, the concentration in the reactor body is ensured to be 10-100 nmol / L; the speed of the agitator during pre-anoxic stirring is 70-100 r / min, and the pre-anoxic stirring time is 100-140 min; the intermittent aeration time is 1260-2700 min, and the aeration volume is 0.3-0.5 L / min; the precipitation time after the reaction is completed is 2 min.
2. A method for rapidly restoring the denitrification performance of a short-cut nitrification-anaerobic ammonium oxidation pilot system according to claim 1, characterized in that: In step 1, the filling volume ratio of the suspended filler is 40% to 50% of the volume of the reactor body; the perforated aeration pipe is connected to the aeration pump through a gas flow meter, the aeration pump is connected to the PLC control system, and the automatic operation of the aeration pump is controlled by the PLC control system.
3. The method for rapidly restoring the denitrification performance of a short-cut nitrification-anaerobic ammonium oxidation pilot system according to claim 1, characterized in that: The agitator is used to stir the mixed liquid in the short-range nitrification-anaerobic ammonium oxidation pilot reactor. The agitator includes a motor and a rotating shaft. A stirring paddle is arranged on the rotating shaft. The motor drives the stirring paddle on the rotating shaft to rotate to fluidize the filler. The heater is used to provide the required temperature to the short-range nitrification-anaerobic ammonium oxidation pilot reactor. The heater includes at least one heating belt wound around the outer wall of the reactor body. The temperature of the reactor body is adjusted by the heating belt. The operating temperature of the reactor body is 30±2°C, and it is operated in the dark.
4. The method for rapidly restoring the denitrification performance of a short-range nitrification-anaerobic ammonium oxidation pilot system according to claim 1, characterized in that: The short-range nitrification-anaerobic ammonium oxidation pilot system also includes a dissolved oxygen probe, a pH probe and a water quality analyzer. The dissolved oxygen probe and the pH probe are both connected to the water quality analyzer. The dissolved oxygen concentration in the reactor body is detected by the dissolved oxygen probe, and the pH in the reactor body is detected by the pH probe. A submersible sewage pump is arranged on the pipeline connecting the water tank and the water inlet of the short-range nitrification-anaerobic ammonium oxidation pilot reactor. The submersible sewage pump and the metering pump are both connected to the PLC control system.
5. The method for rapidly restoring the denitrification performance of a short-cut nitrification-anaerobic ammonium oxidation pilot system according to claim 1, characterized in that: An electric drain valve is arranged on the pipeline connected to the drain outlet, and the electric drain valve is connected to the PLC control system.
Citation Information
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