A fast-switching chromatographic column thermostat and switching method

Abstract

The invention relates to the technical field of column chromatography, and in particular to a fast-switching chromatographic column thermostat and a switching method. The fast-switching chromatographic column thermostat comprises a chromatographic column thermostat body, a first chromatographic column, a second chromatographic column, a first six-way valve, a second six-way valve, a first inlet pipe, a first outlet pipe, a second inlet pipe and a second outlet pipe. The first chromatographic column and the second chromatographic column are arranged so as to test two items separately. By switching the first six-way valve and the second six-way valve to change the flow direction of the solvent, the switching of different connection modes between an automatic sampler and a chromatographic column and between a chromatographic column and a mass spectrometer can be realized, so as to reduce the contamination of the chromatographic column and the mass spectrometer by substances other than an object to be tested during the test and analysis process, and to improve the sensitivity of the detection and analysis and the tolerance and durability of the instrument.

Description

Technical Field

[0001] The invention relates to the technical field of column chromatography, and in particular to a fast-switching chromatographic column thermostat and a switching method. Background Art

[0002] Liquid chromatography-mass spectrometry is a method that utilizes the different distribution and adsorption coefficients of the sample components between the mobile phase and the stationary phase in the chromatographic column. After the sample is carried into the chromatographic column by the mobile phase for separation, it is ionized by the ion source through the interface device to produce ion fragments with a certain charge and mass number. Different ion fragments have different motion behaviors in different electric fields and (or) magnetic fields. The mass analyzer separates the ions according to the mass-to-charge ratio and obtains a mass spectrum arranged in order of mass. By analyzing and processing the mass spectrum, the qualitative and quantitative analysis results of the sample can be obtained.

[0003] In traditional chromatography systems, it is impossible to switch between different connection modes between the automatic sampler and the chromatographic column, and between the chromatographic column and the mass spectrometer, which easily leads to contamination of the chromatographic column and the mass spectrometer by substances other than the analyte during the test and analysis process. Summary of the invention

[0004] The object of the present invention is to provide a fast-switching chromatographic column thermostat and a switching method, which can realize the switching of different connection modes between an automatic sampler and a chromatographic column, and between a chromatographic column and a mass spectrometer, so as to reduce the contamination of the chromatographic column and the mass spectrometer by substances other than the analyte during the test and analysis process, and improve the sensitivity of the detection and analysis and the tolerance and durability of the instrument.

[0005] To achieve the above-mentioned purpose, in a first aspect, the present invention provides a fast-switching chromatographic column thermostat, comprising a chromatographic column thermostat body, a first chromatographic column, a second chromatographic column, a first six-way valve, a second six-way valve, a first inlet pipe, a first outlet pipe, a second inlet pipe and a second outlet pipe; the first chromatographic column is arranged inside the chromatographic column thermostat body; the second chromatographic column is arranged inside the chromatographic column thermostat body; the first six-way valve is arranged on one side of the chromatographic column thermostat body; the second six-way valve is arranged on one side of the chromatographic column thermostat body; the first inlet pipe is connected to the No. 1 position of the first six-way valve, and is connected to the first chromatographic column, and passes through the chromatographic column thermostat body; the first outlet pipe is connected to the first chromatographic column, and is connected to the No. 3 position of the second six-way valve, and passes through the chromatographic column thermostat body; the second inlet pipe is connected to the No. 2 position of the first six-way valve, and is connected to the second chromatographic column, and passes through the chromatographic column thermostat body; the second outlet pipe is connected to the second chromatographic column, and is connected to the No. 1 position of the second six-way valve, and passes through the chromatographic column thermostat body.

[0006] Wherein, the chromatographic column thermostat body comprises a chromatographic column thermostat body, two locking assemblies, a box cover and four plug-in blocks; the two locking assemblies are respectively arranged on the side of the chromatographic column thermostat body; the box cover is located on one side of the chromatographic column thermostat body; the four plug-in blocks are respectively fixedly connected to the box cover and are respectively located on the side of the box cover; the plug-in block has a locking groove; the locking assembly comprises a mounting seat, an elastic telescopic rod, two locking blocks, two push rods and two rebound members; the mounting seat is fixedly connected to the chromatographic column thermostat body and is located on the side of the chromatographic column thermostat body; the mounting seat has two slots; the elastic telescopic rod is fixedly connected to the mounting seat and is located inside the mounting seat; the two locking blocks are respectively slidably connected to the mounting seat, and are respectively fixedly connected to the elastic telescopic rod, and are respectively located inside the mounting seat; the two push rods are respectively fixedly connected to the two locking blocks and pass through the mounting seat respectively; the two rebound members are respectively arranged in the two slots.

[0007] Among them, the elastic telescopic rod includes a sleeve, two sliding rods and a first spring; the sleeve is fixedly connected to the mounting seat and is located inside the mounting seat; the two sliding rods are respectively slidably connected to the sleeve, and are respectively fixedly connected to the two locking blocks, and respectively pass through the sleeve; the first spring is respectively fixedly connected to the two sliding rods and is located in the sleeve.

[0008] Wherein, the push rod includes a connecting rod and a button; the connecting rod is fixedly connected to the locking block and passes through the mounting seat; the button is fixedly connected to the connecting rod and is located on one side of the connecting rod.

[0009] Wherein, the rebound member includes a rebound block and a second spring; the rebound block is slidably connected to the mounting seat and is located in the slot; the second spring is fixedly connected to the rebound block and to the mounting seat, and is located in the slot.

[0010] Among them, the fast-switching chromatographic column thermostat also includes multiple columns and multiple rubber feet; the multiple columns are respectively fixedly connected to the chromatographic column thermostat body and are respectively located on one side of the chromatographic column thermostat body; the multiple rubber feet are respectively fixedly connected to the multiple columns and are respectively located on one side of the multiple columns.

[0011] In a second aspect, the present invention further provides a method for switching a chromatographic column thermostat for rapid switching, when testing reserpine, the specific steps include:

[0012] Connecting the autosampler to position 6 of the first six-way valve, and connecting the mass spectrometer to position 2 of the second six-way valve;

[0013] Connect position 5 of the first six-way valve and position 5 of the second six-way valve to a waste liquid bucket respectively;

[0014] In the 0-2min stage, the first six-way valve is switched to position 5 to allow the liquid to enter the waste liquid pipeline;

[0015] In the 2-3 min stage, the first six-way valve is switched to position 1, and the second six-way valve is switched to position 2, and the sample enters the mass spectrometer through the first chromatographic column;

[0016] In the 3-5min stage, the first six-way valve is switched to position 1, and the second six-way valve is switched to position 3, so that the liquid enters the waste liquid pipeline.

[0017] When testing two projects, the specific steps include:

[0018] Connecting the autosampler to position 6 of the first six-way valve, and connecting the mass spectrometer to position 2 of the second six-way valve;

[0019] Connect position 5 of the first six-way valve and position 5 of the second six-way valve to a waste liquid bucket respectively;

[0020] The first six-way valve is switched to position 1, and the second six-way valve is switched to position 2, and the sample enters the mass spectrometer through the first chromatographic column to achieve the test of the first item;

[0021] When the test of the first item is completed, the first six-way valve is switched from position 1 to position 2, and the test of the second item can be quickly carried out after the pressure of the second chromatographic column is balanced.

[0022] A fast-switching chromatographic column thermostat and switching method of the present invention, wherein the first chromatographic column and the second chromatographic column are arranged to test two items separately, and when testing reserpine, the automatic sample injector is connected to position 6 of the first six-way valve, and the mass spectrometer is connected to position 2 of the second six-way valve; position 5 of the first six-way valve and position 3 of the second six-way valve are respectively connected to a waste liquid bucket; in the 0-2min stage, the first six-way valve is switched to position 5 to enable liquid to enter the waste liquid pipeline; in the 2-3min stage, the first six-way valve is switched to position 1, and the second six-way valve is switched to position 2, and the sample enters the mass spectrometer through the first chromatographic column; in the 3-5min stage, the first six-way valve is switched to position 1, and the second six-way valve is switched to position 3 to enable liquid to enter the waste liquid pipeline; when testing two items, the automatic sample injector is connected to the first six-way valve. The first chromatographic column is connected to position No. 6, and the mass spectrometer is connected to position No. 2 of the second six-way valve; position No. 5 of the first six-way valve and position No. 3 of the second six-way valve are respectively connected to a waste liquid bucket; the first six-way valve is switched to position No. 1, and the second six-way valve is switched to position No. 2, and the sample enters the mass spectrometer through the first chromatographic column to realize the test of the first project; when the test of the first project is completed, the first six-way valve is switched from position No. 1 to position No. 2, and the second chromatographic column can be quickly tested after the pressure is balanced, so as to realize the rapid switching of the two projects, and the flow direction of the solvent is changed by switching the first six-way valve and the second six-way valve, so as to realize the switching of different connection modes between the automatic sampler and the chromatographic column, and between the chromatographic column and the mass spectrometer, so as to reduce the contamination of the chromatographic column and the mass spectrometer by substances other than the analyte during the test and analysis process, and improve the sensitivity of the detection and analysis as well as the tolerance and durability of the instrument. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art are briefly introduced below.

[0024] Figure 1 It is a schematic structural diagram of the first embodiment of the present invention.

[0025] Figure 2 yes Figure 1 A partial enlargement of detail A.

[0026] Figure 3 It is a right side view of the first embodiment of the present invention.

[0027] Figure 4 yes Figure 3 Cross-sectional view along the AA direction.

[0028] Figure 5It is a front view of the first embodiment of the present invention.

[0029] Figure 6 yes Figure 5 Cross-sectional view along direction BB.

[0030] Figure 7 yes Figure 6 A partial enlargement of detail B.

[0031] Figure 8 yes Figure 6 A partial enlargement of detail C.

[0032] Fig. 9 It is a schematic structural diagram of the second embodiment of the present invention.

[0033] Fig.10 It is a schematic structural diagram of the third embodiment of the present invention.

[0034] Fig.11 yes Fig.10 A partial enlargement of detail D.

[0035] Fig.12 is a flow chart of the fourth embodiment of the present invention.

[0036] Fig.13 is a flow chart of the fifth embodiment of the present invention.

[0037] Fig.14 Schematic diagram of the first embodiment of the present invention when in use.

[0038] 101-chromatographic column thermostat body, 102-first chromatographic column, 103-second chromatographic column, 104-first six-way valve, 105-second six-way valve, 106-first inlet pipe, 107-first outlet pipe, 108-second inlet pipe, 109-second outlet pipe, 110-chromatographic column thermostat body, 111-locking assembly, 112-box cover, 113-insert block, 114-mounting seat, 115-elastic telescopic rod, 116 -lock block, 117-push rod, 118-rebound piece, 119-lock slot, 120-slot, 121-sleeve, 122-slide rod, 123-first spring, 124-connecting rod, 125-button, 126-rebound block, 127-second spring, 201-column, 202-rubber pad, 301-support plate, 302-mounting cylinder, 303-screw, 304-knob, 305-mounting plate, 306-support rod. DETAILED DESCRIPTION

[0039] The first embodiment of the present application is:

[0040] See also Figure 1-Figure 8 ,in, Figure 1 It is a schematic structural diagram of the first embodiment of the present invention. Figure 2 yes Figure 1 A partial enlargement of detail A. Figure 3 It is a right side view of the first embodiment of the present invention. Figure 4 yes Figure 3 Cross-sectional view along the AA direction. Figure 5 It is a front view of the first embodiment of the present invention. Figure 6 yes Figure 5 Cross-sectional view along direction BB. Figure 7 yes Figure 6 A partial enlargement of detail B. Figure 8 yes Figure 6 A partial enlargement of detail C. Fig.14 Schematic diagram of the first embodiment of the present invention when in use.

[0041] The present invention provides a fast-switching chromatographic column thermostat, comprising a chromatographic column thermostat body 101, a first chromatographic column 102, a second chromatographic column 103, a first six-way valve 104, a second six-way valve 105, a first inlet pipe 106, a first outlet pipe 107, a second inlet pipe 108 and a second outlet pipe 109; the chromatographic column thermostat body 101 comprises a chromatographic column thermostat body 110, two locking components 111, a box cover 112 and four plug blocks 113; the locking component 111 comprises a mounting seat 114, an elastic telescopic rod 115, two locking blocks 116, two push rods 117 and two rebounding members 118; the The insert block 113 has a locking groove 119; the mounting seat 114 has two slots 120; the elastic telescopic rod 115 includes a sleeve 121, two sliding rods 122 and a first spring 123; the push rod 117 includes a connecting rod 124 and a button 125; the rebound member 118 includes a rebound block 126 and a second spring 127; the above-mentioned scheme can realize the switching of different connection modes between the automatic sampler and the chromatographic column, and between the chromatographic column and the mass spectrometer, so as to reduce the contamination of the chromatographic column and the mass spectrometer by substances other than the object to be tested during the test and analysis process, and improve the sensitivity of the detection and analysis and the tolerance and durability of the instrument.

[0042] According to the present specific embodiment, the first chromatographic column 102 is arranged inside the chromatographic column thermostat body 101; the second chromatographic column 103 is arranged inside the chromatographic column thermostat body 101; the first six-way valve 104 is arranged on one side of the chromatographic column thermostat body 101; the second six-way valve 105 is arranged on one side of the chromatographic column thermostat body 101; the first inlet pipe 106 is connected to the No. 1 position of the first six-way valve 104, and is connected to the first chromatographic column 102, and passes through the chromatographic column thermostat body 101. 01; the first outlet pipe 107 is connected to the first chromatographic column 102, and is connected to the No. 3 position of the second six-way valve 105, and passes through the chromatographic column thermostat body 101; the second inlet pipe 108 is connected to the No. 2 position of the first six-way valve 104, and is connected to the second chromatographic column 103, and passes through the chromatographic column thermostat body 101; the second outlet pipe 109 is connected to the second chromatographic column 103, and is connected to the No. 1 position of the second six-way valve 105, and passes through the chromatographic column thermostat body 101.The chromatographic column thermostat body 101 is a chromatographic column thermostat in the prior art, and the first chromatographic column 102 and the second chromatographic column 103 are clamped and installed inside. The first chromatographic column 102 and the second chromatographic column 103 are arranged to test two items separately. When testing reserpine, the automatic sampler is connected to the No. 6 position of the first six-way valve 104, and the mass spectrometer is connected to the No. 2 position of the second six-way valve 105; the No. 5 position of the first six-way valve 104 and the No. 3 position of the second six-way valve 105 are connected separately. In the 0-2min stage, the first six-way valve 104 is switched to position 5, so that the liquid enters the waste liquid pipeline; in the 2-3min stage, the first six-way valve 104 is switched to position 1, and the second six-way valve 105 is switched to position 2, and the sample enters the mass spectrometer through the first chromatographic column 102; in the 3-5min stage, the first six-way valve 104 is switched to position 1, and the second six-way valve 105 is switched to position 3, so that the liquid enters the waste liquid pipeline; when testing two items At this time, the automatic sampler is connected to the No. 6 position of the first six-way valve 104, and the mass spectrometer is connected to the No. 2 position of the second six-way valve 105; the No. 5 position of the first six-way valve 104 and the No. 3 position of the second six-way valve 105 are respectively connected to a waste liquid bucket; the first six-way valve 104 is switched to the No. 1 position, and the second six-way valve 105 is switched to the No. 2 position, and the sample enters the mass spectrometer through the first chromatographic column 102 to achieve the test of the first project; when the test of the first project is completed, the first six-way valve 104 is switched from the No. 1 position to the No. 2 position, and the second project can be tested quickly after the pressure of the second chromatographic column 103 is balanced, so as to achieve rapid switching of the two projects, and the flow direction of the solvent is changed by switching the first six-way valve 104 and the second six-way valve 105, so as to achieve the switching of different connection modes between the automatic sampler and the chromatographic column, and between the chromatographic column and the mass spectrometer, so as to reduce the contamination of the chromatographic column and the mass spectrometer by substances other than the analyte during the test and analysis process, and improve the sensitivity of the detection and analysis and the tolerance and durability of the instrument.

[0043] The two locking components 111 are respectively arranged on the side of the chromatographic column thermostat body 110; the box cover 112 is located on one side of the chromatographic column thermostat body 110; the four plug blocks 113 are respectively fixedly connected to the box cover 112 and are respectively located on the side of the box cover 112; the plug blocks 113 have locking grooves 119; the locking component 111 includes a mounting seat 114, an elastic telescopic rod 115, two locking blocks 116, two push rods 117 and two rebounding members 118; the mounting seat 114 is fixedly connected to the chromatographic column thermostat body 110 and is located at the The side of the chromatography column thermostat body 110; the mounting seat 114 has two slots 120; the elastic telescopic rod 115 is fixedly connected to the mounting seat 114 and is located inside the mounting seat 114; the two locking blocks 116 are respectively slidably connected to the mounting seat 114, and are respectively fixedly connected to the elastic telescopic rod 115, and are respectively located inside the mounting seat 114; the two push rods 117 are respectively fixedly connected to the two locking blocks 116, and respectively pass through the mounting seat 114; the two rebound members 118 are respectively arranged in the two slots 120. The two locking assemblies 111 cooperate with the four plugs 113 to fix the box cover 112 on the side of the chromatographic column thermostat main body 110; two plugs 113 are arranged on the left and right sides of the box cover 112, the plugs 113 are adapted to the slots 120, the locking blocks 116 are adapted to the locking grooves 119, the ends of the locking blocks 116 have inclined surfaces, the elastic telescopic rods 115 can be shortened by force, and when the external force disappears, the elongated recovery is restored, the rebounding member 118 is elastic, and moving the push rod 117 can drive the locking blocks 116 to move; when installing the box cover 112, align the plugs 113 with the slots 120 and insert them, at which time the plugs 113 will squeeze the locking blocks 116 along the inclined surfaces of the locking blocks 116, and the locking blocks 116 The elastic telescopic rod 115 is squeezed, and at the same time, the plug block 113 will also press the rebound member 118, and the plug block 113 is continuously inserted until the locking slot 119 is aligned with the locking block 116. The elastic telescopic rod 115 pushes the locking block 116 to be inserted into the locking slot 119 to lock the plug block 113. When removing the box cover 112, the two push rods 117 on the left and right sides are pressed at the same time, so that the two push rods 117 are close to each other, and the two locking blocks 116 on the same side are also close to each other, so that all the locking blocks 116 are withdrawn from the corresponding locking slots 119. At this time, under the action of the rebound member 118, the plug block 113 can be bounced a distance from the slot 120, and the box cover 112 can be removed.

[0044] Secondly, the sleeve 121 is fixedly connected to the mounting seat 114 and is located inside the mounting seat 114; the two slide bars 122 are respectively slidably connected to the sleeve 121 and are respectively fixedly connected to the two locking blocks 116 and pass through the sleeve 121; the first spring 123 is respectively fixedly connected to the two slide bars 122 and is located inside the sleeve 121. The sleeve 121 and the two slide bars 122 form a telescopic rod. When the two locking blocks 116 are squeezed and slid, the two slide bars 122 slide into the sleeve 121, thereby compressing the first spring 123. When the two locking blocks 116 are no longer subjected to external force, the first spring 123 pushes the two slide bars 122, thereby driving the two locking blocks 116 to reset.

[0045] At the same time, the connecting rod 124 is fixedly connected to the locking block 116 and passes through the mounting seat 114; the button 125 is fixedly connected to the connecting rod 124 and is located on one side of the connecting rod 124. The connecting rod 124 connects the button 125 and the locking block 116. Pressing the button 125 can drive the locking block 116 to slide. When removing the box cover 112, the two buttons 125 on the left and right sides are pressed at the same time, so that the two connecting rods 124 are close to each other. At this time, the two locking blocks 116 on the same side are also close to each other, so that all the locking blocks 116 are withdrawn from the corresponding locking slots 119. At this time, under the action of the rebound member 118, the insert block 113 can be bounced a distance from the slot 120, and the box cover 112 can be removed.

[0046] In addition, the rebound block 126 is slidably connected to the mounting seat 114 and is located in the slot 120; the second spring 127 is fixedly connected to the rebound block 126 and the mounting seat 114 and is located in the slot 120. When the insert block 113 is pressed down, the rebound block 126 presses down the second spring 127, and after the locking block 116 no longer locks the insert block 113, the second spring 127 drives the rebound block 126 to bounce the insert block 113 from the slot 120 for a distance.

[0047] The embodiment of the present invention discloses a fast-switching chromatographic column thermostat, wherein the chromatographic column thermostat body 101 is a chromatographic column thermostat in the prior art, and the first chromatographic column 102 and the second chromatographic column 103 are clamped and installed inside. The first chromatographic column 102 and the second chromatographic column 103 are arranged so as to test two items separately. By switching the first six-way valve 104 and the second six-way valve 105 to change the flow direction of the solvent, different connection modes between the automatic sampler and the chromatographic column and between the chromatographic column and the mass spectrometer can be switched, so as to reduce the contamination of the chromatographic column and the mass spectrometer by substances other than the analyte during the test and analysis process, and to improve the sensitivity of the detection and analysis and the tolerance and durability of the instrument. The two locking assemblies 111 cooperate with the four plugs 113 to fix the box cover 112 on the side of the chromatographic column thermostat main body 110; two plugs 113 are arranged on the left and right sides of the box cover 112, the plugs 113 are adapted to the slots 120, the locking blocks 116 are adapted to the locking grooves 119, the ends of the locking blocks 116 have inclined surfaces, the elastic telescopic rods 115 can be shortened by force, and when the external force disappears, the elongated recovery is restored, the rebounding member 118 is elastic, and moving the push rod 117 can drive the locking blocks 116 to move; when installing the box cover 112, align the plugs 113 with the slots 120 and insert them, at which time the plugs 113 will squeeze the locking blocks 116 along the inclined surfaces of the locking blocks 116, and the locking blocks 116 The elastic telescopic rod 115 is squeezed, and at the same time, the plug block 113 will also press the rebound member 118, and the plug block 113 is continuously inserted until the locking slot 119 is aligned with the locking block 116. The elastic telescopic rod 115 pushes the locking block 116 to be inserted into the locking slot 119 to lock the plug block 113. When removing the box cover 112, the two push rods 117 on the left and right sides are pressed at the same time, so that the two push rods 117 are close to each other, and the two locking blocks 116 on the same side are also close to each other, so that all the locking blocks 116 are withdrawn from the corresponding locking slots 119. At this time, under the action of the rebound member 118, the plug block 113 can be bounced a distance from the slot 120, and the box cover 112 can be removed. It should be noted that the different positions of the second six-way valve 105 are connected by the rotation of the circular rotor in the middle; the main function of the No. 6 position in the middle of the first six-way valve 104 is to inject the sample to be tested into the first six-way valve 104; the sample is input into the first chromatographic column 102, or the second chromatographic column 103 or into the waste liquid bucket by the rotation of the circular rotor in the middle of the first six-way valve 104. The main function of the second six-way valve 105 is to switch the sample flowing from the first chromatographic column 102 or the second chromatographic column 103, inject it into the mass spectrometer, or switch it to the waste liquid bucket.

[0048] The second embodiment of the present application is:

[0049] Based on the first embodiment, please refer to Fig. 9 ,in, Fig. 9 It is a schematic structural diagram of the second embodiment of the present invention.

[0050] The fast-switching chromatographic column thermostat provided by the present invention further includes a plurality of columns 201 and a plurality of rubber feet 202 .

[0051] According to this specific embodiment, the plurality of columns 201 are respectively fixedly connected to the chromatographic column thermostat body 110 and are respectively located on one side of the chromatographic column thermostat body 110; the plurality of rubber feet 202 are respectively fixedly connected to the plurality of columns 201 and are respectively located on one side of the plurality of columns 201. The plurality of columns 201 support the chromatographic column thermostat body 110 to a certain height, and the plurality of rubber feet 202 are provided for anti-slip, so that the chromatographic column thermostat body 110 can be placed horizontally.

[0052] In the fast-switching chromatographic column thermostat described in this embodiment, a plurality of the upright posts 201 support the chromatographic column thermostat body 110 to a certain height, and a plurality of the rubber feet 202 are provided for anti-slipping, so that the chromatographic column thermostat body 110 can be placed horizontally.

[0053] The third embodiment of the present application is:

[0054] Based on the second embodiment, please refer to Figure 10-11 ,in, Fig.10 It is a schematic structural diagram of the third embodiment of the present invention. Fig.11 yes Fig.10 A partial enlargement of detail D.

[0055] The fast-switching chromatographic column thermostat provided by the present invention further includes a plurality of support plates 301 , a mounting tube 302 , a screw rod 303 , a knob 304 , a mounting plate 305 and a plurality of support rods 306 .

[0056] According to the present specific embodiment, the plurality of support plates 301 are rotatably connected to the chromatographic column thermostat body 110, and are respectively located on one side of the chromatographic column thermostat body 110; the mounting tube 302 is fixedly connected to the chromatographic column thermostat body 110, and is located on one side of the chromatographic column thermostat body 110; the screw 303 is threadedly connected to the mounting tube 302, and is located inside the mounting tube 302; the knob 304 is fixedly connected to the screw 303, and is located on one side of the screw 303; the mounting disk 305 is rotatably connected to the screw 303, and is penetrated by the screw 303; the plurality of support rods 306 are rotatably connected to the mounting disk 305, and are respectively rotatably connected to the plurality of support plates 301, and are respectively located on the sides of the mounting disk 305. The mounting plate 305 and the screw rod 303 can only rotate relative to each other and cannot slide relative to each other. When the chromatographic column thermostat body 110 needs to be placed vertically, the knob 304 can be turned to move the screw rod 303 into the mounting tube 302, thereby driving the mounting plate 305 to move. The mounting plate 305 then drives the multiple support plates 301 to unfold through the multiple support rods 306. At this time, the chromatographic column thermostat body 110 can be supported by the multiple support plates 301 to place the chromatographic column thermostat body 110 vertically.

[0057] In the fast-switching chromatographic column thermostat described in this embodiment, the mounting disk 305 and the screw rod 303 can only rotate relative to each other and cannot slide relative to each other. When the chromatographic column thermostat body 110 needs to be placed vertically, the knob 304 can be rotated to move the screw rod 303 into the mounting tube 302, thereby driving the mounting disk 305 to move. The mounting disk 305 then drives the multiple support plates 301 to unfold through the multiple support rods 306. At this time, the chromatographic column thermostat body 110 can be supported by the multiple support plates 301 to place the chromatographic column thermostat body 110 vertically.

[0058] The fourth embodiment of the present application is:

[0059] See also Fig.12 ,in, Fig.12 is a flow chart of the fourth embodiment of the present invention.

[0060] The present invention provides a method for switching a chromatographic column thermostat for rapid switching. When testing reserpine, the specific steps include:

[0061] S1 connects the autosampler to position 6 of the first six-way valve 104 and connects the mass spectrometer to position 2 of the second six-way valve 105;

[0062] S2 connects the No. 5 position of the first six-way valve 104 and the No. 5 position of the second six-way valve 105 to a waste liquid bucket respectively;

[0063] S3: In the 0-2min stage, the first six-way valve 104 is switched to position 5 to allow the liquid to enter the waste liquid pipeline;

[0064] S4: In the 2-3min stage, the first six-way valve 104 is switched to position 1, and the second six-way valve 105 is switched to position 2, and the sample enters the mass spectrometer through the first chromatographic column 102;

[0065] S5 In the 3-5min stage, the first six-way valve 104 is switched to position 1, and the second six-way valve 105 is switched to position 3, so that the liquid enters the waste liquid pipeline.

[0066] The six-way switching valve structure consists of a base, a rotor, and a stator. The rotor is located between the base and the stator, and the flow path is switched by the rotation of the stator. The sample is sampled by an automatic sampler, and then driven into the first six-way valve 104 by the mobile phase. Assuming that the retention time of substance 1 in the sample when passing through the first chromatographic column 102 (which is the established chromatographic analysis condition) is 2.5 minutes, then before 2.5 minutes, there is no detection chromatographic peak of substance 1 in the detection instrument, and this part of the mobile phase can be switched to waste liquid. Since the time when the chromatographic peak of substance 1 is detected is 2.25 minutes from 2.75 minutes to 2.75 minutes (peak width 0.5 minutes), there is no chromatographic peak of substance 1 after 3 minutes. The flow is switched by the second six-way valve 105 to realize the liquid (mobile phase) entering the waste liquid, which can reduce the contamination of the detector (mass spectrometer).

[0067] The fifth embodiment of the present application is:

[0068] See also Fig.13 ,in, Fig.13 is a flow chart of the fifth embodiment of the present invention.

[0069] The present invention provides a fast switching method for switching a chromatographic column thermostat, when testing two items, the specific steps include:

[0070] S1 connects the autosampler to position 6 of the first six-way valve 104 and connects the mass spectrometer to position 2 of the second six-way valve 105;

[0071] S2 connects the No. 5 position of the first six-way valve 104 and the No. 5 position of the second six-way valve 105 to a waste liquid bucket respectively;

[0072] S3: Switch the first six-way valve 104 to position 1, and switch the second six-way valve 105 to position 2. The sample enters the mass spectrometer through the first chromatographic column 102 to achieve the test of the first item.

[0073] S4 When the first item test is completed, the first six-way valve 104 is switched from position 1 to position 2, and the second item test can be quickly performed after the pressure of the second chromatographic column 103 is balanced.

[0074] The detection instrument is a liquid chromatography tandem mass spectrometry system, in which the liquid chromatography part is equipped with a two-element pump (A pump and pump), and each liquid phase pump is equipped with two sets of flow (such as A1 and A2, B1 and B2).

[0075] (1) During the project detection and analysis, the mobile phases used are A1 and B1, and the chromatographic column used is the first chromatographic column 102.

[0076] (2) When the item 2 is tested and analyzed, after the item 1 is tested, the mobile phase is switched (A2 and B2) by software settings, and the liquid phase of item 2 is started. After the pressure of the second chromatographic column 103 is balanced (about 10 minutes), the automatic sampler starts the test program "sampling - entering the second chromatographic column 103 for separation - then entering the mass spectrometer" for detection (at this time, the substance 2 is detected)

[0077] (3) The detection and analysis conditions (mobile phase composition, mobile phase gradient, mobile phase gradient time setting, chromatographic column, etc.) of different detection items (such as hormone items and amino acid items) are different, so different detection parameter conditions need to be set when the same sample is tested for two items. The online switching of "the first chromatographic column 102" and "the second chromatographic column 103" can achieve rapid switching of different detection items.

[0078] What is disclosed above is only one or more preferred embodiments of the present application, and cannot be used to limit the scope of rights of the present application. Ordinary technicians in this field can understand that all or part of the processes of implementing the above embodiments and equivalent changes made according to the claims of the present application are still within the scope covered by the present application.

Claims

1. A fast-switching chromatographic column thermostat, characterized in that: The invention comprises a chromatographic column thermostat body, a first chromatographic column, a second chromatographic column, a first six-way valve, a second six-way valve, a first inlet pipe, a first outlet pipe, a second inlet pipe and a second outlet pipe; the first chromatographic column is arranged inside the chromatographic column thermostat body; the second chromatographic column is arranged inside the chromatographic column thermostat body; the first six-way valve is arranged on one side of the chromatographic column thermostat body; the second six-way valve is arranged on one side of the chromatographic column thermostat body; the first inlet pipe is communicated with the No. 1 position of the first six-way valve, and is communicated with the first chromatographic column, and passes through the chromatographic column thermostat body; the first outlet pipe is communicated with the first chromatographic column, and is communicated with the No. 3 position of the second six-way valve, and passes through the chromatographic column thermostat body; the second inlet pipe is communicated with the No. 2 position of the first six-way valve, and is communicated with the second chromatographic column, and passes through the chromatographic column thermostat body; the second outlet pipe is communicated with the second chromatographic column, and is communicated with the No. 1 position of the second six-way valve, and passes through the chromatographic column thermostat body; The chromatographic column thermostat body comprises a chromatographic column thermostat body, two locking assemblies, a box cover and four plug-in blocks; the two locking assemblies are respectively arranged on the side of the chromatographic column thermostat body; the box cover is located on one side of the chromatographic column thermostat body; the four plug-in blocks are respectively fixedly connected to the box cover and are respectively located on the side of the box cover; the plug-in block has a locking groove; the locking assembly comprises a mounting seat, an elastic telescopic rod, two locking blocks, two push rods and two rebound members; the mounting seat is fixedly connected to the chromatographic column thermostat body and is located on the side of the chromatographic column thermostat body; the mounting seat has two slots; the elastic telescopic rod is fixedly connected to the mounting seat and is located inside the mounting seat; the two locking blocks are respectively slidably connected to the mounting seat, and are respectively fixedly connected to the elastic telescopic rod, and are respectively located inside the mounting seat; the two push rods are respectively fixedly connected to the two locking blocks and pass through the mounting seat respectively; the two rebound members are respectively arranged in the two slots; The elastic telescopic rod includes a sleeve, two sliding rods and a first spring; the sleeve is fixedly connected to the mounting seat and is located inside the mounting seat; the two sliding rods are respectively slidably connected to the sleeve, and are respectively fixedly connected to the two locking blocks, and respectively pass through the sleeve; the first spring is respectively fixedly connected to the two sliding rods and is located in the sleeve.

2. A fast-switching chromatographic column thermostat as claimed in claim 1, characterized in that: The push rod includes a connecting rod and a button; the connecting rod is fixedly connected to the locking block and passes through the mounting seat; the button is fixedly connected to the connecting rod and is located on one side of the connecting rod.

3. A fast-switching chromatographic column thermostat as claimed in claim 2, characterized in that: The rebound member includes a rebound block and a second spring; the rebound block is slidably connected to the mounting seat and is located in the slot; the second spring is fixedly connected to the rebound block and to the mounting seat, and is located in the slot.

4. A fast-switching chromatographic column thermostat as claimed in claim 3, characterized in that: The fast-switching chromatographic column thermostat also includes a plurality of columns and a plurality of rubber feet; the plurality of columns are respectively fixedly connected to the chromatographic column thermostat body and are respectively located on one side of the chromatographic column thermostat body; the plurality of rubber feet are respectively fixedly connected to the plurality of columns and are respectively located on one side of the plurality of columns.

5. A method for switching a fast-switching chromatographic column thermostat, applied to a fast-switching chromatographic column thermostat as claimed in any one of claims 1 to 4, characterized in that ； When testing reserpine, the specific steps include: Connecting the autosampler to position 6 of the first six-way valve, and connecting the mass spectrometer to position 2 of the second six-way valve; Connect position 5 of the first six-way valve and position 5 of the second six-way valve to a waste liquid bucket respectively; In the 0-2min stage, the first six-way valve is switched to position 5 to allow the liquid to enter the waste liquid pipeline; In the 2-3 min stage, the first six-way valve is switched to position 1, and the second six-way valve is switched to position 2, and the sample enters the mass spectrometer through the first chromatographic column; In the 3-5min stage, the first six-way valve is switched to position 1, and the second six-way valve is switched to position 3, so that the liquid enters the waste liquid pipeline.

6. A method for switching a fast-switching chromatographic column thermostat, applied to a fast-switching chromatographic column thermostat as claimed in any one of claims 1 to 4, characterized in that ； When testing two projects, the specific steps include: Connecting the autosampler to position 6 of the first six-way valve, and connecting the mass spectrometer to position 2 of the second six-way valve; Connect position 5 of the first six-way valve and position 5 of the second six-way valve to a waste liquid bucket respectively; The first six-way valve is switched to position 1, and the second six-way valve is switched to position 2, and the sample enters the mass spectrometer through the first chromatographic column to achieve the test of the first item; When the test of the first item is completed, the first six-way valve is switched from position 1 to position 2, and the test of the second item can be quickly carried out after the pressure of the second chromatographic column is balanced.

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