Toothpaste composition containing plant extract and preparation method thereof

Abstract

The invention provides a toothpaste composition containing a plant extract and a preparation method thereof, and belongs to the technical field of cosmetics, the toothpaste composition comprises the following components: a humectant, a plant extract, a friction agent, a preservative, a thickening agent, a surfactant, a flavoring agent, a synergistic additive and water; wherein the humectant is a mixture consisting of glycerol and D-mannitol; the plant extract is obtained by taking pericarpium zanthoxyli peel, whole herb of sarcandra glabra and whole herb of selfheal as plant raw materials through enzymolysis, microbial fermentation and extraction. The abrasive is a mixture of calcium carbonate and silicon dioxide; the synergistic auxiliary agent is a mixture of nano hydroxyapatite and nano magnesium aluminum silicate. According to the toothpaste composition prepared by the invention, the plant extracts are adopted to replace traditional chemical medicine components, so that side effects caused by long-term use of the chemical medicine components can be avoided, and the toothpaste composition has excellent and stable effects of inhibiting bacteria, diminishing inflammation, cleaning, whitening and the like.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and in particular relates to a toothpaste composition containing plant extracts and a preparation method thereof. Background Art

[0002] As an important daily oral care product, toothpaste can be traced back to ancient times when people used simple abrasives and natural substances to clean their teeth. With the advancement of technology, modern toothpaste not only has a cleaning function, but also integrates a variety of functional ingredients to provide people with comprehensive oral care. Most existing oral care products usually contain ingredients such as chemical drugs and fluoride, which are widely used in toothpaste to achieve analgesic, anti-inflammatory and anti-caries effects. For example, fluoride can enhance the mineralization of the tooth surface and effectively prevent tooth decay, while antibiotics and other chemical drugs are used to control gingival inflammation and relieve pain.

[0003] However, long-term dependence on these chemical ingredients may bring a series of irreversible side effects. For example, excessive intake of fluoride may cause fluorosis and affect the appearance of teeth. Some chemical drugs may trigger allergic reactions or destroy the microbial balance in the mouth, leading to aggravated oral health problems. In contrast, oral care products with natural plant extracts such as tea tree oil, mint, aloe vera, honeysuckle, and two-sided needles as active ingredients not only provide efficient cleaning, but also reduce the risks brought by chemicals. These plant extracts have natural antibacterial, anti-inflammatory and soothing properties, which can promote overall oral health while maintaining oral hygiene, satisfying people's demand for a more gentle, safe and environmentally friendly choice. Through scientific proportions, toothpaste containing plant extracts can not only keep the mouth fresh, but also effectively prevent and treat common oral diseases, and gradually become an ideal substitute for traditional chemical toothpaste.

[0004] At present, although adding plant extracts to make toothpaste and other oral care products has many advantages mentioned above, there are still some limitations and challenges. For example, the efficacy of plant extract formulas is usually mild, and its therapeutic effect on oral problems such as severe caries or acute gingivitis may not be as obvious as traditional toothpaste containing strong chemical drugs; the active ingredients of plant extracts used on the market have relatively poor stability, and are easily affected by light, temperature and humidity during storage and use, causing the product to lose some of its effectiveness; in addition, some plant extracts such as tea tree oil, although they have good antibacterial effects, their strong odor may make people feel uncomfortable, and flavors or other flavorings need to be added. Therefore, there is an urgent need for a new toothpaste composition containing plant extracts, which has excellent and stable antibacterial and anti-inflammatory, cleaning and whitening effects. Summary of the invention

[0005] The purpose of the present invention is to provide a toothpaste composition containing plant extracts. By adding homemade plant extracts and specific synergistic additives, together with components such as moisturizers and abrasives, the toothpaste composition is made into a more gentle and safe one, avoiding the side effects caused by long-term use of chemical drug ingredients, and at the same time having excellent and stable antibacterial and anti-inflammatory, cleaning and whitening effects.

[0006] In order to achieve the above object, the present invention provides the following technical solutions:

[0007] The present invention provides a toothpaste composition containing a plant extract, comprising the following components:

[0008] 25-35 parts by weight of moisturizer, 5-8 parts by weight of plant extract, 25-30 parts by weight of abrasive, 0.4-0.6 parts by weight of preservative, 1-2 parts by weight of thickener, 0.5-1.5 parts by weight of surfactant, 1-2 parts by weight of flavoring agent, 2-4 parts by weight of synergistic aid, and 30-35 parts by weight of water.

[0009] Preferably, the moisturizing agent is one or a mixture of two or more of glycerol, 1,2-propylene glycol, 1,4-butylene glycol, D-mannitol, and polyethylene glycol.

[0010] Furthermore, the moisturizing agent is a mixture of glycerol and D-mannitol in a weight ratio of 6-8:1.

[0011] Preferably, the friction agent is one or a mixture of two or more of calcium carbonate, calcium hydrogen phosphate, calcium pyrophosphate, silicon dioxide, hydroxyapatite, and aluminum hydroxide.

[0012] Furthermore, the friction agent is a mixture of calcium carbonate and silicon dioxide in a weight ratio of 1-3:1.

[0013] Preferably, the preservative is one or a mixture of two or more of phenoxyethanol, sodium benzoate, potassium sorbate, p-hydroxyacetophenone, ethylhexylglycerin, and benzalkonium chloride.

[0014] Furthermore, the preservative is phenoxyethanol.

[0015] Preferably, the thickener is one or a mixture of two or more of xanthan gum, sodium carboxymethyl cellulose, sodium alginate, carbomer, xanthan gum, and hydroxyethyl cellulose.

[0016] Furthermore, the thickener is a mixture of xanthan gum, sodium carboxymethyl cellulose and sodium alginate in a weight ratio of 1-3:1-3:1.

[0017] Preferably, the surfactant is one or a mixture of two or more of sodium lauryl sulfate, sodium N-lauroyl sarcosinate, sodium laureth sulfate, cocamidopropyl betaine, and sodium methyl cocoyl taurate.

[0018] Furthermore, the surfactant is a mixture of sodium lauryl sulfate and sodium N-lauroyl sarcosinate in a weight ratio of 3-5:1.

[0019] Preferably, the flavoring agent is one or a mixture of two or more of xylitol, sorbitol, menthol, sucralose, and sodium saccharin.

[0020] Furthermore, the flavoring agent is xylitol.

[0021] The toothpaste composition of the present invention is prepared by adding homemade plant extracts instead of traditional chemical drug ingredients, together with components such as moisturizers, abrasives, preservatives, thickeners, surfactants, and flavoring agents, which can not only avoid the side effects caused by long-term use of chemical drug ingredients, but also have excellent and stable antibacterial and cleaning and whitening effects. This is because the homemade plant extract of the present invention is processed from Zanthoxylum bungeanum peel, whole grass coral grass, and whole grass Prunella vulgaris as raw materials, these plant raw materials are combined with each other, and then sequentially undergo enzymolysis, microbial fermentation, alcohol extraction, water extraction, and other steps to obtain an extract rich in multiple active ingredients, including flavonoids, polyphenols, terpenes, vitamins, alkaloids, and active polysaccharides, etc., which has excellent antibacterial, antioxidant, and anti-inflammatory effects.

[0022] During the preparation process, the plant cell walls are first decomposed by cellulase and bromelain through warm water soaking and enzymatic hydrolysis steps, thereby releasing more effective ingredients and enhancing the extraction efficiency. Subsequently, isopropanol is added for reflux extraction to fully dissolve the active ingredients contained in the plant raw materials. In order to further improve the utilization rate of the plant raw materials, specific microorganisms Bacillus amyloliquefaciens and Lactobacillus mucilaginosus are used to ferment and extract the filter residue. This process not only allows the active ingredients remaining in the filter residue to be dissolved and extracted again, but also produces more active metabolites such as antimicrobial peptides, small molecule polysaccharides, etc. These substances have good antibacterial activity and antioxidant capacity, further enhancing the antibacterial effect of the toothpaste.

[0023] Among them, flavonoids and polyphenol compounds are natural antioxidants that can remove free radicals and prevent oxidative stress from damaging tooth enamel. They can also chelate metal ions and reduce pigment deposition on the tooth surface, thereby whitening teeth. In addition, these ingredients can inhibit the growth of harmful bacteria in the mouth, especially streptococci, actinomycetes and Candida albicans that cause caries and periodontal disease. Terpenoid compounds have strong antibacterial and anti-inflammatory properties and can effectively kill or inhibit pathogenic bacteria in the mouth, reduce gingival inflammation and bad breath problems. Alkaloids further enhance the antibacterial effect of toothpaste by interfering with the metabolic pathways of bacteria and inhibiting their reproduction. Antimicrobial peptides can directly destroy bacterial cell walls, leading to bacterial death and providing long-lasting antibacterial protection. Small molecule polysaccharides and polypeptides have good moisturizing and film-forming properties and can form a protective film on the tooth surface to prevent the invasion of external pollutants and bacteria, while keeping the mouth moist and reducing gingival dryness and bleeding. In summary, the plant extract of the present invention not only avoids the side effects of traditional chemical drugs through the synergistic effect of multiple active ingredients, but also provides stable and efficient antibacterial and whitening effects, and together constitutes a comprehensive oral care system.

[0024] Preferably, the method for preparing the plant extract comprises the following steps:

[0025] The plant raw material is crushed and sieved to obtain plant raw material powder; 50-70 parts by weight of the plant raw material powder is added to 100-300 parts by weight of water, heated and stirred, and then 0.2-0.5 parts by weight of cellulase and 0.1-0.4 parts by weight of bromelain are added for enzymolysis, and then 200-400 parts by weight of isopropanol are added for reflux extraction and filtering to obtain filter residue and filtrate; the filtrate is concentrated under reduced pressure to obtain a primary extract; the filter residue is dried, crushed and sieved to obtain filter residue powder;

[0026] 8-12 parts by weight of filter residue powder, 1-2 parts by weight of glucose, 0.4-0.6 parts by weight of yeast extract, 0.5-1.5 parts by weight of peptone, 0.2-0.4 parts by weight of sodium chloride, 0.1-0.3 parts by weight of dipotassium hydrogen phosphate and 80-100 parts by weight of water are mixed, sterilized, inoculated with microorganisms after cooling, and fermented. After the fermentation is completed, sterilized, cooled, filtered, and the filtrate is collected, and the filtrate is concentrated under reduced pressure to obtain a secondary extract; 3-5 parts by weight of the primary extract and 2-3 parts by weight of the secondary extract are evenly mixed to obtain a plant extract.

[0027] Furthermore, the method for preparing the plant extract comprises the following steps:

[0028] The plant raw material is crushed and sieved through a 100-150 mesh sieve to obtain a plant raw material powder; 50-70 parts by weight of the plant raw material powder is added to 100-300 parts by weight of water, stirred at 50-55° C. and 100-150 r / min for 0.5-2 hours, then 0.2-0.5 parts by weight of cellulase and 0.1-0.4 parts by weight of bromelain are added, the pH is adjusted to 5.5-6.5, enzymolysis is performed at 50-55° C. for 10-15 hours, then 200-400 parts by weight of isopropanol are added, the temperature is raised to 80-90° C., reflux extraction is performed for 5-7 hours, and filtering is performed to obtain a filter residue and a filtrate; the filtrate is concentrated under reduced pressure to 4-6% of the original volume to obtain a primary extract; the filter residue is dried, crushed, and sieved through a 100-150 mesh sieve to obtain a filter residue powder;

[0029] 8-12 parts by weight of filter residue powder, 1-2 parts by weight of glucose, 0.4-0.6 parts by weight of yeast extract, 0.5-1.5 parts by weight of peptone, 0.2-0.4 parts by weight of sodium chloride, 0.1-0.3 parts by weight of dipotassium hydrogen phosphate and 80-100 parts by weight of water are mixed to obtain a liquid, which is sterilized at 115-125° C. for 10-30 min, and inoculated with microorganisms after cooling to room temperature. The inoculation amount of the microorganisms is 1×10 8 -3×10 8 cfu / mL liquid, ferment at a constant temperature of 35-40°C for 40-60h, sterilize at 115-125°C for 10-30min after the fermentation, cool to room temperature, filter, collect the filtrate, and concentrate the filtrate under reduced pressure to 4-6% of the original volume to obtain a secondary extract; 3-5 parts by weight of the primary extract and 2-3 parts by weight of the secondary extract are evenly mixed to obtain a plant extract.

[0030] Preferably, the plant raw material is composed of Zanthoxylum bungeanum peel, whole grass coral herb and whole grass selfheal herb in a weight ratio of 1:0.4-0.6:0.4-0.6;

[0031] Preferably, the plant raw material is washed and dried for later use, and its water content is controlled within 6%.

[0032] Preferably, the microorganisms are composed of Bacillus amyloliquefaciens and Lactobacillus mucilaginosus fermentation, and the usage ratio of Bacillus amyloliquefaciens to Lactobacillus mucilaginosus fermentation is 1-3:3.

[0033] Preferably, the number of Bacillus amyloliquefaciens is CI CC 20605, and the number of Lactobacillus mucilaginosus fermentation is CI CC22823, both of which are purchased from China Industrial Microbiological Culture Collection Center.

[0034] Preferably, the synergistic aid is nano hydroxyapatite and / or nano magnesium aluminum silicate.

[0035] The present invention also adds a synergistic auxiliary agent composed of nano-hydroxyapatite and nano-magnesium aluminum silicate to the toothpaste composition, which can further improve the cleaning and whitening effect. Among them, nano-hydroxyapatite is a bioactive material similar to the main components of human teeth, has a high specific surface area and good biocompatibility, can deeply penetrate into the tiny pores and cracks on the surface of teeth, fill these defects, repair damaged enamel, enhance the hardness and gloss of teeth, and can also remove pigment deposition and dental plaque on the surface of teeth through adsorption and chelation, thereby achieving a whitening effect; and nano-magnesium aluminum silicate has good adsorption performance and mild friction, which can prevent pollutants and harmful microorganisms from adhering to the surface of teeth, and at the same time through mild mechanical friction. , effectively removes stains and tartar on the surface of teeth without damaging the enamel. In addition, it has good lubricity, which can reduce irritation to the gums during brushing, keep the mouth moist, and reduce gum bleeding and dryness. Nano-hydroxyapatite and nano-magnesium aluminum silicate work together. Nano-hydroxyapatite strengthens the tooth structure and removes pigments and impurities from the inside through chemical chelation and adsorption, while nano-magnesium aluminum silicate removes stains and tartar from the outside through physical friction and adsorption. This dual mechanism not only improves the cleaning efficiency, but also enhances the whitening effect, making teeth smoother and whiter.

[0036] Furthermore, the synergistic aid is a mixture of nano-hydroxyapatite and nano-magnesium aluminum silicate in a weight ratio of 1-3:1.

[0037] The present invention provides a method for preparing a toothpaste composition containing a plant extract, comprising the following steps:

[0038] Accurately weigh each component according to the component formula, mix the moisturizer and the plant extract with water, stir for 15-30 minutes at 50-70 r / min, then add the abrasive and thickener and stir for 5-15 minutes, then add the preservative, surfactant, flavoring agent and synergist and stir for 10-30 minutes, and vacuum degas for 10-30 minutes at a vacuum degree of -0.09 to -0.1 MPa to obtain the toothpaste composition containing the plant extract.

[0039] Compared with the prior art, the advantages and beneficial effects of the present invention are:

[0040] 1. The present invention provides a method for preparing a toothpaste composition containing plant extracts, which is prepared by adding plant extracts obtained by enzymolysis and microbial fermentation of Zanthoxylum bungeanum peel, whole grass coral and whole grass Prunella vulgaris as plant raw materials, and a synergistic auxiliary agent composed of nano-hydroxyapatite and nano-magnesium aluminum silicate, and a moisturizer, abrasive and other components to prepare a toothpaste composition, which is more gentle and safe, avoids the side effects caused by long-term use of chemical drug ingredients, and also has excellent and stable antibacterial and anti-inflammatory, cleaning and whitening effects. The toothpaste composition provided by the present invention is simple and easy to operate.

[0041] 2. The toothpaste composition of the present invention is made by adding homemade plant extracts instead of traditional chemical drug ingredients, together with moisturizers, abrasives, preservatives, thickeners, surfactants, flavoring agents and other components, and has excellent and stable antibacterial and cleaning and whitening effects. This is because the homemade plant extract of the present invention is processed from Zanthoxylum bungeanum peel, whole grass coral grass and whole grass Prunella vulgaris as raw materials. These plant raw materials are combined with each other, and then successively undergo enzymolysis, microbial fermentation, alcohol extraction and water extraction steps to obtain an extract rich in various active ingredients, including flavonoids, polyphenols, terpenes, vitamins, alkaloids and active polysaccharides, etc., which have excellent antibacterial, antioxidant and anti-inflammatory effects.

[0042] 3. The present invention further adds a synergistic agent composed of nano-hydroxyapatite and nano-magnesium aluminum silicate to the toothpaste composition, which can further improve the cleaning and whitening effect. Among them, nano-hydroxyapatite and nano-magnesium aluminum silicate work together. Nano-hydroxyapatite strengthens the tooth structure and removes pigments and impurities from the inside through chemical chelation and adsorption, while nano-magnesium aluminum silicate removes stains and tartar from the outside through physical friction and adsorption. This dual mechanism not only improves the cleaning efficiency, but also enhances the whitening effect, making the teeth smoother and whiter. DETAILED DESCRIPTION

[0043] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0044] Example 1

[0045] This embodiment provides a toothpaste composition containing plant extracts, comprising the following components:

[0046] 30 parts by weight of moisturizer, 6.5 parts by weight of plant extract, 28 parts by weight of abrasive, 0.5 parts by weight of preservative, 1.5 parts by weight of thickener, 1 part by weight of surfactant, 1.5 parts by weight of flavoring agent, 3 parts by weight of synergistic aid, 32 parts by weight of water. The moisturizer is a mixture of glycerol and D-mannitol in a weight ratio of 7:1. The abrasive is a mixture of calcium carbonate and silicon dioxide in a weight ratio of 2:1; wherein the average particle size of calcium carbonate is 5 μm; and the average particle size of silicon dioxide is 10 μm. The preservative is phenoxyethanol. The thickener is a mixture of xanthan gum, sodium carboxymethyl cellulose and sodium alginate in a weight ratio of 2:2:1. The surfactant is a mixture of sodium dodecyl sulfate and sodium N-lauroyl sarcosine in a weight ratio of 4:1. The flavoring agent is xylitol. The synergistic aid is a mixture of nano-hydroxyapatite and nano-magnesium aluminum silicate in a weight ratio of 2:1; wherein the average particle size of the nano-hydroxyapatite is 20nm, and the average particle size of the nano-magnesium aluminum silicate is 100nm.

[0047] The preparation method of the plant extract comprises the following steps:

[0048] The plant raw material is crushed and sieved through a 125-mesh sieve to obtain a plant raw material powder; 60 parts by weight of the plant raw material powder is added to 200 parts by weight of water, stirred at 53°C and 120 r / min for 1 hour, then 0.35 parts by weight of cellulase and 0.25 parts by weight of bromelain are added, the pH is adjusted to 6, enzymolysis is carried out at 53°C for 12 hours, and then 300 parts by weight of isopropanol are added, the temperature is raised to 85°C for reflux extraction for 6 hours, and filtered to obtain a filter residue and a filtrate; the filtrate is concentrated under reduced pressure to 5% of the original volume to obtain a primary extract; the filter residue is dried and crushed and sieved through a 125-mesh sieve to obtain a filter residue powder; the plant raw material is composed of Zanthoxylum bungeanum peel, whole grass coral herb and whole grass selfheal herb in a weight ratio of 1:0.5:0.5; wherein the plant raw material is washed and dried for standby use, and its water content is controlled within 6%; the enzyme activity of the cellulase is 50u / mg; the enzyme activity of the bromelain is 600u / mg;

[0049] 10 parts by weight of filter residue powder, 1.5 parts by weight of glucose, 0.5 parts by weight of yeast extract, 1 part by weight of peptone, 0.3 parts by weight of sodium chloride, 0.2 parts by weight of dipotassium hydrogen phosphate and 90 parts by weight of water were mixed to obtain a feed solution, which was sterilized at 121° C. for 15 min and inoculated with microorganisms after cooling to room temperature. The inoculation amount of the microorganisms was 2.2×10 8cfu / mL liquid, ferment at a constant temperature of 37°C for 48h, sterilize at 121°C for 15min after fermentation, cool to room temperature, filter, collect the filtrate, and concentrate the filtrate under reduced pressure to 5% of the original volume to obtain a secondary extract; 4 parts by weight of the primary extract and 2.5 parts by weight of the secondary extract are mixed evenly to obtain a plant extract. The microorganism is composed of Bacillus amyloliquefaciens and fermented Lactobacillus mucus, and the dosage ratio of the Bacillus amyloliquefaciens to the fermented Lactobacillus mucus is 2:3; wherein, the number of Bacillus amyloliquefaciens is CICC 20605, and the number of fermented Lactobacillus mucus is CI CC 22823, both of which are purchased from the China Industrial Microbiological Culture Collection Management Center.

[0050] This embodiment provides a method for preparing a toothpaste composition containing a plant extract, comprising the following steps:

[0051] Accurately weigh each component according to the component formula, mix the moisturizer and plant extract with water, stir at 60r / min for 20 minutes, then add the abrasive and thickener and stir for 10 minutes, then add the preservative, surfactant, flavoring agent and synergist and stir for 20 minutes, and vacuum degas for 20 minutes at a vacuum degree of -0.096MPa to obtain the toothpaste composition containing the plant extract.

[0052] Example 2

[0053] This embodiment provides a toothpaste composition containing plant extracts, comprising the following components:

[0054] 25 parts by weight of moisturizer, 5 parts by weight of plant extract, 25 parts by weight of abrasive, 0.4 parts by weight of preservative, 1 part by weight of thickener, 0.5 parts by weight of surfactant, 1 part by weight of flavoring agent, 2 parts by weight of synergistic aid, 30 parts by weight of water. The moisturizer is a mixture of glycerol and D-mannitol in a weight ratio of 6:1. The abrasive is a mixture of calcium carbonate and silicon dioxide in a weight ratio of 1:1; wherein the average particle size of calcium carbonate is 5 μm; and the average particle size of silicon dioxide is 10 μm. The preservative is phenoxyethanol. The thickener is a mixture of xanthan gum, sodium carboxymethyl cellulose and sodium alginate in a weight ratio of 1:1:1. The surfactant is a mixture of sodium dodecyl sulfate and sodium N-lauroyl sarcosine in a weight ratio of 3:1. The flavoring agent is xylitol. The synergistic aid is a mixture of nano-hydroxyapatite and nano-magnesium aluminum silicate in a weight ratio of 1:1; wherein the average particle size of the nano-hydroxyapatite is 20nm, and the average particle size of the nano-magnesium aluminum silicate is 100nm.

[0055] The preparation method of the plant extract is the same as that in Example 1.

[0056] This embodiment provides a method for preparing a toothpaste composition containing a plant extract, comprising the following steps:

[0057] Accurately weigh each component according to the component formula, mix the moisturizer and plant extract with water, stir for 15 minutes at 50r / min, then add the abrasive and thickener and stir for 5 minutes, then add the preservative, surfactant, flavoring agent and synergist and stir for 10 minutes, and vacuum degas for 10 minutes at a vacuum degree of -0.09MPa to obtain the toothpaste composition containing the plant extract.

[0058] Example 3

[0059] This embodiment provides a toothpaste composition containing plant extracts, comprising the following components:

[0060] 35 parts by weight of moisturizer, 8 parts by weight of plant extract, 30 parts by weight of abrasive, 0.45 parts by weight of preservative, 2 parts by weight of thickener, 1.5 parts by weight of surfactant, 2 parts by weight of flavoring agent, 4 parts by weight of synergistic aid, 35 parts by weight of water. The moisturizer is a mixture of glycerol and D-mannitol in a weight ratio of 8:1. The abrasive is a mixture of calcium carbonate and silicon dioxide in a weight ratio of 3:1; wherein the average particle size of calcium carbonate is 5 μm; and the average particle size of silicon dioxide is 10 μm. The preservative is phenoxyethanol. The thickener is a mixture of xanthan gum, sodium carboxymethyl cellulose and sodium alginate in a weight ratio of 3:3:1. The surfactant is a mixture of sodium dodecyl sulfate and sodium N-lauroyl sarcosine in a weight ratio of 5:1. The flavoring agent is xylitol. The synergistic aid is a mixture of nano-hydroxyapatite and nano-magnesium aluminum silicate in a weight ratio of 3:1; wherein the average particle size of the nano-hydroxyapatite is 20nm, and the average particle size of the nano-magnesium aluminum silicate is 100nm.

[0061] The preparation method of the plant extract is the same as that in Example 1.

[0062] This embodiment provides a method for preparing a toothpaste composition containing a plant extract, comprising the following steps:

[0063] Accurately weigh each component according to the component formula, mix the moisturizer and plant extract with water, stir at 70r / min for 30 minutes, then add the abrasive and thickener and stir for 15 minutes, then add the preservative, surfactant, flavoring agent and synergist and stir for 30 minutes, and vacuum degas for 30 minutes at a vacuum degree of -0.1MPa to obtain the toothpaste composition containing the plant extract.

[0064] Comparative Example 1

[0065] The difference between this comparative example and Example 1 is that the preparation method of the plant extract is different, which is as follows: The preparation method of the plant extract comprises the following steps:

[0066] The plant raw material is crushed and sieved through a 125-mesh sieve to obtain a plant raw material powder; 60 parts by weight of the plant raw material powder is added to 200 parts by weight of water, stirred at 53°C and 120 r / min for 1 hour, then 0.35 parts by weight of cellulase and 0.25 parts by weight of bromelain are added, the pH is adjusted to 6, enzymolysis is carried out at 53°C for 12 hours, and then 300 parts by weight of isopropanol are added, the temperature is raised to 85°C for reflux extraction for 6 hours, and filtered to obtain a filter residue and a filtrate; the filtrate is concentrated under reduced pressure to 5% of the original volume to obtain a primary extract; the filter residue is dried, crushed, and sieved through a 125-mesh sieve to obtain a filter residue powder; the plant raw material is composed of Zanthoxylum bungeanum peel and whole grass coral grass in a weight ratio of 1:1; wherein the plant raw material is washed and dried for standby use, and its water content is controlled within 6%; the enzyme activity of the cellulase is 50u / mg; the enzyme activity of the bromelain is 600u / mg;

[0067] 10 parts by weight of filter residue powder, 1.5 parts by weight of glucose, 0.5 parts by weight of yeast extract, 1 part by weight of peptone, 0.3 parts by weight of sodium chloride, 0.2 parts by weight of dipotassium hydrogen phosphate and 90 parts by weight of water were mixed to obtain a feed solution, which was sterilized at 121° C. for 15 min and inoculated with microorganisms after cooling to room temperature. The inoculation amount of the microorganisms was 2.2×10 8 cfu / mL liquid, ferment at a constant temperature of 37°C for 48h, sterilize at 121°C for 15min after fermentation, cool to room temperature, filter, collect the filtrate, and concentrate the filtrate under reduced pressure to 5% of the original volume to obtain a secondary extract; 4 parts by weight of the primary extract and 2.5 parts by weight of the secondary extract are mixed evenly to obtain a plant extract. The microorganism is composed of Bacillus amyloliquefaciens and fermented Lactobacillus mucus, and the dosage ratio of the Bacillus amyloliquefaciens to the fermented Lactobacillus mucus is 2:3; wherein, the number of Bacillus amyloliquefaciens is CICC 20605, and the number of fermented Lactobacillus mucus is CI CC 22823, both of which are purchased from the China Industrial Microbiological Culture Collection Management Center.

[0068] Comparative Example 2

[0069] The difference between this comparative example and Example 1 is that the preparation method of the plant extract is different, which is as follows: The preparation method of the plant extract comprises the following steps:

[0070] The plant raw material is crushed and sieved through a 125-mesh sieve to obtain a plant raw material powder; 60 parts by weight of the plant raw material powder is added to 200 parts by weight of water, stirred at 53°C and 120r / min for 1h, then 0.35 parts by weight of cellulase and 0.25 parts by weight of bromelain are added, the pH is adjusted to 6, enzymolysis is carried out at 53°C for 12h, 300 parts by weight of isopropanol are added, the temperature is raised to 85°C for reflux extraction for 6h, and filtered to obtain a filter residue and a filtrate; the filtrate is concentrated under reduced pressure to 5% of the original volume to obtain a primary extract; the filter residue is dried and crushed and sieved through a 125-mesh sieve to obtain a filter residue powder; the plant raw material is composed of Zanthoxylum bungeanum peel and Prunella vulgaris whole grass in a weight ratio of 1:1; wherein the plant raw material is washed and dried for standby use, and its water content is controlled within 6%; the enzyme activity of the cellulase is 50u / mg; the enzyme activity of the bromelain is 600u / mg;

[0071] 10 parts by weight of filter residue powder, 1.5 parts by weight of glucose, 0.5 parts by weight of yeast extract, 1 part by weight of peptone, 0.3 parts by weight of sodium chloride, 0.2 parts by weight of dipotassium hydrogen phosphate and 90 parts by weight of water were mixed to obtain a feed solution, which was sterilized at 121° C. for 15 min and inoculated with microorganisms after cooling to room temperature. The inoculation amount of the microorganisms was 2.2×10 8 cfu / mL liquid, ferment at a constant temperature of 37°C for 48h, sterilize at 121°C for 15min after fermentation, cool to room temperature, filter, collect the filtrate, and concentrate the filtrate under reduced pressure to 5% of the original volume to obtain a secondary extract; 4 parts by weight of the primary extract and 2.5 parts by weight of the secondary extract are mixed evenly to obtain a plant extract. The microorganism is composed of Bacillus amyloliquefaciens and fermented Lactobacillus mucus, and the dosage ratio of the Bacillus amyloliquefaciens to the fermented Lactobacillus mucus is 2:3; wherein, the number of Bacillus amyloliquefaciens is CICC 20605, and the number of fermented Lactobacillus mucus is CI CC 22823, both of which are purchased from the China Industrial Microbiological Culture Collection Management Center.

[0072] Comparative Example 3

[0073] The difference between this comparative example and Example 1 is that the preparation method of the plant extract is different, which is as follows: The preparation method of the plant extract comprises the following steps:

[0074] The plant raw material is crushed and sieved through a 125-mesh screen to obtain a plant raw material powder; 60 parts by weight of the plant raw material powder is added to 200 parts by weight of water, stirred at 53°C and 120r / min for 1h, then 0.35 parts by weight of cellulase and 0.25 parts by weight of bromelain are added, the pH is adjusted to 6, enzymolysis is performed at 53°C for 12h, 300 parts by weight of isopropanol are added, the temperature is raised to 85°C for reflux extraction for 6h, filtered, and a filter residue and a filtrate are obtained; the filtrate is concentrated under reduced pressure to 5% of the original volume to obtain a plant extract. The plant raw material is composed of Zanthoxylum bungeanum peel, whole grass coral grass and whole grass Prunella vulgaris in a weight ratio of 1:0.5:0.5; wherein, the plant raw material is washed and dried for standby use, and its water content is controlled within 6%; the enzyme activity of the cellulase is 50u / mg; the enzyme activity of the bromelain is 600u / mg.

[0075] Comparative Example 4

[0076] The difference between this comparative example and Example 1 is that the preparation method of the plant extract is different, which is as follows: The preparation method of the plant extract comprises the following steps:

[0077] The plant raw material is crushed and sieved through a 125-mesh sieve to obtain a plant raw material powder; the plant raw material is composed of Zanthoxylum bungeanum peel, whole grass coral herb and whole grass selfheal herb in a weight ratio of 1:0.5:0.5; wherein the plant raw material is washed and dried for standby use, and its water content is controlled within 6%;

[0078] 10 parts by weight of plant raw material powder, 1.5 parts by weight of glucose, 0.5 parts by weight of yeast extract, 1 part by weight of peptone, 0.3 parts by weight of sodium chloride, 0.2 parts by weight of dipotassium hydrogen phosphate and 90 parts by weight of water were mixed to obtain a liquid, which was sterilized at 121° C. for 15 min, cooled to room temperature and inoculated with microorganisms. The inoculation amount of the microorganisms was 2.2×10 8 cfu / mL feed liquid, fermented at a constant temperature of 37°C for 48h, sterilized at 121°C for 15min after fermentation, cooled to room temperature, filtered, collected the filtrate, and concentrated the filtrate under reduced pressure to 5% of the original volume to obtain a plant extract. The microorganisms are composed of Bacillus amyloliquefaciens and fermented Lactobacillus mucus, and the dosage ratio of the Bacillus amyloliquefaciens to the fermented Lactobacillus mucus is 2:3; wherein the number of Bacillus amyloliquefaciens is CI CC 20605, and the number of fermented Lactobacillus mucus is CI CC 22823, both of which are purchased from the China Industrial Microbiological Culture Collection Management Center.

[0079] Comparative Example 5

[0080] The difference between this comparative example and Example 1 is that the preparation method of the plant extract is different, which is as follows: The preparation method of the plant extract comprises the following steps:

[0081] The plant raw material is crushed and sieved through a 125-mesh sieve to obtain a plant raw material powder; 60 parts by weight of the plant raw material powder is added to 200 parts by weight of water, stirred at 53°C and 120 r / min for 1 hour, then 0.35 parts by weight of cellulase and 0.25 parts by weight of bromelain are added, the pH is adjusted to 6, enzymolysis is carried out at 53°C for 12 hours, and then 300 parts by weight of isopropanol are added, the temperature is raised to 85°C for reflux extraction for 6 hours, and filtered to obtain a filter residue and a filtrate; the filtrate is concentrated under reduced pressure to 5% of the original volume to obtain a primary extract; the filter residue is dried and crushed and sieved through a 125-mesh sieve to obtain a filter residue powder; the plant raw material is composed of Zanthoxylum bungeanum peel, whole grass coral herb and whole grass selfheal herb in a weight ratio of 1:0.5:0.5; wherein the plant raw material is washed and dried for standby use, and its water content is controlled within 6%; the enzyme activity of the cellulase is 50u / mg; the enzyme activity of the bromelain is 600u / mg;

[0082] 10 parts by weight of filter residue powder, 1.5 parts by weight of glucose, 0.5 parts by weight of yeast extract, 1 part by weight of peptone, 0.3 parts by weight of sodium chloride, 0.2 parts by weight of dipotassium hydrogen phosphate and 90 parts by weight of water were mixed to obtain a feed solution, which was sterilized at 121° C. for 15 min and inoculated with microorganisms after cooling to room temperature. The inoculation amount of the microorganisms was 2.2×10 8 cfu / mL liquid, fermented at a constant temperature of 37°C for 48h, sterilized at 121°C for 15min after fermentation, cooled to room temperature, filtered, collected the filtrate, and concentrated the filtrate under reduced pressure to 5% of the original volume to obtain a secondary extract; 4 parts by weight of the primary extract and 2.5 parts by weight of the secondary extract were mixed evenly to obtain a plant extract. The microorganism is Bacillus amyloliquefaciens; wherein, Bacillus amyloliquefaciens is numbered CI CC 20605 and purchased from the China Industrial Microbiological Culture Collection Management Center.

[0083] Comparative Example 6

[0084] The difference between this comparative example and Example 1 is that the preparation method of the plant extract is different, which is as follows: The preparation method of the plant extract comprises the following steps:

[0085] The plant raw material is crushed and sieved through a 125-mesh sieve to obtain a plant raw material powder; 60 parts by weight of the plant raw material powder is added to 200 parts by weight of water, stirred at 53°C and 120 r / min for 1 hour, then 0.35 parts by weight of cellulase and 0.25 parts by weight of bromelain are added, the pH is adjusted to 6, enzymolysis is carried out at 53°C for 12 hours, and then 300 parts by weight of isopropanol are added, the temperature is raised to 85°C for reflux extraction for 6 hours, and filtered to obtain a filter residue and a filtrate; the filtrate is concentrated under reduced pressure to 5% of the original volume to obtain a primary extract; the filter residue is dried and crushed and sieved through a 125-mesh sieve to obtain a filter residue powder; the plant raw material is composed of Zanthoxylum bungeanum peel, whole grass coral herb and whole grass selfheal herb in a weight ratio of 1:0.5:0.5; wherein the plant raw material is washed and dried for standby use, and its water content is controlled within 6%; the enzyme activity of the cellulase is 50u / mg; the enzyme activity of the bromelain is 600u / mg;

[0086] 10 parts by weight of filter residue powder, 1.5 parts by weight of glucose, 0.5 parts by weight of yeast extract, 1 part by weight of peptone, 0.3 parts by weight of sodium chloride, 0.2 parts by weight of dipotassium hydrogen phosphate and 90 parts by weight of water were mixed to obtain a feed solution, which was sterilized at 121° C. for 15 min and inoculated with microorganisms after cooling to room temperature. The inoculation amount of the microorganisms was 2.2×10 8 cfu / mL feed liquid, fermented at a constant temperature of 37°C for 48h, sterilized at 121°C for 15min after fermentation, cooled to room temperature, filtered, collected the filtrate, and concentrated the filtrate under reduced pressure to 5% of the original volume to obtain a secondary extract; 4 parts by weight of the primary extract and 2.5 parts by weight of the secondary extract were mixed evenly to obtain a plant extract. The microorganism is fermented mucus lactobacillus; wherein the fermented mucus lactobacillus is numbered CI CC 22823 and purchased from the China Industrial Microbiological Culture Collection Management Center.

[0087] Comparative Example 7

[0088] The difference between this comparative example and Example 1 is that the synergistic aid is nano-hydroxyapatite; wherein the average particle size of the nano-hydroxyapatite is 20 nm.

[0089] Comparative Example 8

[0090] The difference between this comparative example and Example 1 is that the synergistic aid is nano magnesium aluminum silicate; wherein the average particle size of the nano magnesium aluminum silicate is 100 nm.

[0091] Performance Testing

[0092] 1. The toothpaste compositions containing plant extracts obtained in the above Examples 1-3 and Comparative Examples 1-8 were placed under corresponding conditions for a period of time, specifically as follows: 3±2°C for 45 days, 23±2°C for 45 days, and 55±2°C for 45 days, and the toothpaste compositions were observed to see whether they changed color, precipitated, or produced odor. If no color change, precipitation, or odor occurred, it means that the storage stability test was "passed". On the contrary, if at least one of color change, precipitation, or odor occurred, it means that the storage stability test was "failed". The results are shown in Table 1.

[0093] Table 1: Storage stability test results

[0094] 3±2℃ 23±2℃ 55±2℃ Example 1 pass pass pass Example 2 pass pass pass Example 3 pass pass pass Comparative Example 1 pass pass Not passed Comparative Example 2 pass pass Not passed Comparative Example 3 pass Not passed Not passed Comparative Example 4 pass Not passed Not passed Comparative Example 5 pass pass pass Comparative Example 6 pass pass pass Comparative Example 7 pass pass pass Comparative Example 8 pass pass pass

[0095] 2. The antibacterial rates of the toothpaste compositions containing plant extracts obtained in Examples 1-3 and Comparative Examples 1-8 were determined according to standard QB / T 2738-2012, wherein the test bacteria were as follows: Staphylococcus aureus (ATCC 27217), Escherichia coli (ATCC 11229), and Candida albicans (ATCC 10231).

[0096] The bovine incisor was cut into enamel blocks (size 5mm×5mm×2mm), and then embedded in polymethyl methacrylate resin. The lip surface of the enamel block was polished with 180-mesh sandpaper (moistened with water), and the enamel surface of the arc structure was polished flat, washed with water, and then polished smooth with 600-mesh sandpaper (moistened with water), and washed with water. The polished enamel block was immersed in 1wt% hydrochloric acid for 1min, washed with water after being taken out, and then immersed in a saturated sodium carbonate aqueous solution for 1min, washed with water after being taken out, and finally immersed in a 1wt% phytic acid aqueous solution for 1min, washed with water after being taken out. The acid-treated tooth enamel blocks were immersed in a dye for 12 days (the composition of the dye was as follows: based on 1L of water, 2.5g / L gastric mucin, 4.0g / L coffee, 4.0g / L soy sauce, 4.0g / L black tea, and 0.05g / L ferric chloride; ferric chloride solution stored at 4°C was added before use). The color of the stained tooth enamel blocks was measured with a colorimeter (L* 刷牙前), select enamel blocks with L* values ​​of 30-50, randomly divide them into 11 groups, 5 blocks in each group, and carry out subsequent simulated toothbrushing tests. The toothpaste compositions containing plant extracts obtained in the above Examples 1-3 and Comparative Examples 1-8 are mixed evenly with water in a weight ratio of 1:1.5, as a sample toothpaste slurry for standby use. The above-mentioned dyed enamel blocks are placed in the sample fixing groove of the automatic mechanical toothbrush machine and fixed so that the tooth grinding block is about 2mm higher, and tighten the screws to fix it. Install the toothbrush head so that the bristles are in vertical contact with the enamel surface. The total weight of the toothbrush head and rod is 150g. Weigh 70g of the above-mentioned sample toothpaste slurry and add it to the grinding liquid bottle. Fix the grinding liquid bottle on the toothbrush machine so that the slurry buries the enamel block, and brush back and forth 800 times. After brushing, take out the enamel block, wash it with water, dry it, and measure the color of the enamel block after brushing (L* 刷牙后 ), and calculated the difference in enamel color before and after brushing (recorded as △L*). The results are shown in Table 2.

[0097] Table 2: Antibacterial and whitening test results

[0098]

[0099] It can be seen from the above test results that the toothpaste compositions containing plant extracts prepared in Examples 1-3 have good storage stability, antibacterial effect, and cleaning and whitening effect, especially the toothpaste composition corresponding to Example 1 has the most outstanding comprehensive performance. This is because the present invention can effectively improve the antibacterial effect and whitening effect of the toothpaste composition by adding homemade plant extracts and synergistic additives.

[0100] In comparison, since Comparative Examples 1-8 did not adopt the necessary technical solutions, they were significantly worse than Examples 1-3 in the corresponding performance tests. In Comparative Examples 1-6, the plant extracts were not prepared by a specific method, and in Comparative Examples 7-8, no specific combination of synergistic aids were used. It can be seen from the results that this led to a decrease in the comprehensive performance of the toothpaste composition. The above experimental results further prove the importance of the technical solutions defined in the present invention for its technical effects.

[0101] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A toothpaste composition containing a plant extract, characterized in that: Includes the following components: 25-35 parts by weight of moisturizer, 5-8 parts by weight of plant extract, 25-30 parts by weight of abrasive, 0.4-0.6 parts by weight of preservative, 1-2 parts by weight of thickener, 0.5-1.5 parts by weight of surfactant, 1-2 parts by weight of flavor corrective, 2-4 parts by weight of synergistic aid, and 30-35 parts by weight of water; the plant extract is extracted from Zanthoxylum bungeanum peel, whole grass coral herb and whole grass selfheal as plant raw materials after enzymatic hydrolysis and microbial fermentation; the synergistic aid is a mixture of nano-hydroxyapatite and nano-magnesium aluminum silicate.

2. The toothpaste composition containing plant extract according to claim 1, characterized in that: The preparation method of the plant extract comprises the following steps: The plant raw material is crushed and sieved to obtain plant raw material powder; 50-70 parts by weight of the plant raw material powder is added to 100-300 parts by weight of water, heated and stirred, and then 0.2-0.5 parts by weight of cellulase and 0.1-0.4 parts by weight of bromelain are added for enzymolysis, and then 200-400 parts by weight of isopropanol are added for reflux extraction and filtering to obtain filter residue and filtrate; the filtrate is concentrated under reduced pressure to obtain a primary extract; the filter residue is dried, crushed and sieved to obtain filter residue powder; the plant raw material consists of Zanthoxylum bungeanum peel, whole grass coral herb and whole grass selfheal herb; 8-12 parts by weight of filter residue powder, 1-2 parts by weight of glucose, 0.4-0.6 parts by weight of yeast extract, 0.5-1.5 parts by weight of peptone, 0.2-0.4 parts by weight of sodium chloride, 0.1-0.3 parts by weight of dipotassium hydrogen phosphate and 80-100 parts by weight of water are mixed, sterilized, inoculated with microorganisms after cooling, and fermented. After the fermentation is completed, sterilized, cooled, filtered, and the filtrate is collected, and the filtrate is concentrated under reduced pressure to obtain a secondary extract; 3-5 parts by weight of the primary extract and 2-3 parts by weight of the secondary extract are evenly mixed to obtain a plant extract.

3. The toothpaste composition containing plant extract according to claim 2, characterized in that: The preparation method of the plant extract comprises the following steps: The plant raw material is crushed and sieved through a 100-150 mesh screen to obtain a plant raw material powder; 50-70 parts by weight of the plant raw material powder is added to 100-300 parts by weight of water, stirred at 50-55° C. and 100-150 r / min for 0.5-2 h, then 0.2-0.5 parts by weight of cellulase and 0.1-0.4 parts by weight of bromelain are added, the pH is adjusted to 5.5-6.5, and enzymolysis is performed at 50-55° C. for 1 h. 0-15h, then add 200-400 parts by weight of isopropanol, heat to 80-90°C, reflux extraction for 5-7h, filter to obtain filter residue and filtrate; decompress and concentrate the filtrate to 4-6% of the original volume to obtain a primary extract; dry and crush the filter residue and sieve it through a 100-150 mesh sieve to obtain a filter residue powder; the plant raw material is composed of Zanthoxylum bungeanum peel, whole grass coral herb and whole grass selfheal herb in a weight ratio of 1:0.4-0.6:0.4-0.6; 8-12 parts by weight of filter residue powder, 1-2 parts by weight of glucose, 0.4-0.6 parts by weight of yeast extract, 0.5-1.5 parts by weight of peptone, 0.2-0.4 parts by weight of sodium chloride, 0.1-0.3 parts by weight of dipotassium hydrogen phosphate and 80-100 parts by weight of water are mixed to obtain a liquid, which is sterilized at 115-125° C. for 10-30 min, and inoculated with microorganisms after cooling to room temperature. The inoculation amount of the microorganisms is 1×10 8 -3×10 8 cfu / mL liquid, ferment at a constant temperature of 35-40°C for 40-60h, sterilize at 115-125°C for 10-30min after the fermentation, cool to room temperature, filter, collect the filtrate, and concentrate the filtrate under reduced pressure to 4-6% of the original volume to obtain a secondary extract; 3-5 parts by weight of the primary extract and 2-3 parts by weight of the secondary extract are evenly mixed to obtain a plant extract.

4. The toothpaste composition containing plant extract according to claim 2 or 3, characterized in that: The microorganisms are composed of Bacillus amyloliquefaciens and Lactobacillus mucilaginosus fermentation, and the usage ratio of Bacillus amyloliquefaciens to Lactobacillus mucilaginosus fermentation is 1-3:

3.

5. The toothpaste composition containing plant extract according to claim 1, characterized in that: The moisturizing agent is a mixture of glycerol and D-mannitol in a weight ratio of 6-8:

1.

6. The toothpaste composition containing plant extract according to claim 1, characterized in that: The friction agent is a mixture of calcium carbonate and silicon dioxide in a weight ratio of 1-3:

1.

7. The toothpaste composition containing plant extracts according to claim 1, characterized in that: The preservative is one or a mixture of two or more of phenoxyethanol, sodium benzoate, potassium sorbate, p-hydroxyacetophenone, ethylhexylglycerin, and benzalkonium chloride; the flavoring agent is one or a mixture of two or more of xylitol, sorbitol, menthol, sucralose, and sodium saccharin.

8. The toothpaste composition containing plant extracts according to claim 1, characterized in that: The thickener is a mixture of xanthan gum, sodium carboxymethyl cellulose and sodium alginate in a weight ratio of 1-3:1-3:

1.

9. The toothpaste composition containing plant extracts according to claim 1, characterized in that: The surfactant is a mixture of sodium lauryl sulfate and sodium N-lauroyl sarcosinate in a weight ratio of 3-5:

1.

10. The method for preparing a toothpaste composition containing a plant extract according to any one of claims 1 to 9, characterized in that: The steps include: Accurately weigh each component according to the component formula, mix the moisturizer and the plant extract with water, stir for 15-30 minutes at 50-70 r / min, then add the abrasive and thickener and stir for 5-15 minutes, then add the preservative, surfactant, flavoring agent and synergist and stir for 10-30 minutes, and vacuum degas for 10-30 minutes at a vacuum degree of -0.09 to -0.1 MPa to obtain the toothpaste composition containing the plant extract.

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