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Process for preparing bioactive peptide-silk fibroin peptide

A technology of silk fibroin peptide and enzymatic hydrolysis solution, which is applied in the field of food biology, can solve the problems of serious membrane pollution and difficulty in realizing industrial production, and achieve the effects of high desalination rate, large processing capacity and simple process

Inactive Publication Date: 2005-12-28
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the desalination process, membrane technologies such as ultrafiltration and electrodialysis are usually used for desalination, but the membrane pollution of this method is very serious, and it is difficult to realize industrial production

Method used

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  • Process for preparing bioactive peptide-silk fibroin peptide
  • Process for preparing bioactive peptide-silk fibroin peptide
  • Process for preparing bioactive peptide-silk fibroin peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Preparation of silk fibroin peptide powder with degree of hydrolysis (DH)=12%

[0019] Waste silk (nodding) treated with 0.5% (w / v) Na 2 CO 3 After the solution is refined, place it in boiling 40% (w / w) CaCl 2 Dissolve in the solution for 5 minutes and 30 seconds (according to 100ml CaCl 2 The solution dissolves 14 g of scoured silk for dissolving), and cools rapidly. The silk-dissolving solution was filtered through gauze to obtain a concentrated silk-dissolving solution. Take 8 liters of the concentrated silk-dissolving solution, add 12 liters of water, carry out enzymatic reaction with Alcalase enzyme at pH 8.5 and 60°C, and use the pH-stat method to control the degree of hydrolysis. After the reaction is completed, the enzyme is heated to inactivate, centrifuged and filtered, and the filtrate is desalted with a polyamide nanofiltration membrane. During the desalination process, water is continuously added to dilute the feed solution, and the desalted feed solutio...

Embodiment 2

[0041] Preparation of silk fibroin peptide powder with degree of hydrolysis (DH)=10%

[0042] Waste silk (nodding) treated with 0.5% (w / v) Na 2 CO 3 After the solution is refined, place it in boiling 40% (w / w) CaCl 2 Dissolve in the solution for 5 minutes and 30 seconds (according to 100ml CaCl 2 The solution dissolves 14 g of scoured silk for dissolving), and cools rapidly. The silk-dissolving solution was filtered through gauze to obtain a concentrated silk-dissolving solution. Take 7 liters of the concentrated silk-dissolving solution, add 7 liters of water, carry out enzymatic reaction with Alcalase enzyme at pH 8.5 and 60°C, and use the pH-stat method to control the degree of hydrolysis. After the reaction is over, heat to inactivate the enzyme, and after centrifugation and filtration, the filtrate is desalted with a polyethersulfone nanofiltration membrane. During the desalination process, water is continuously added to dilute the feed solution. After desalination, th...

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Abstract

The invention discloses a method for preparing biologically active peptide-silk fibroin peptide, which belongs to the field of food biotechnology. In the present invention, silkworm cocoons or waste silk are used as raw materials, and 0.3-1.0% (w / v) Na 2 CO 3 After the solution is refined, use 20-60% (w / w) CaCl 2 The solution is heated and dissolved to obtain the silk-dissolving liquid, and the silk-dissolving liquid is hydrolyzed with protease at pH 2.5-12.0 and 30-80°C, and then the high-concentration CaCl in the enzymatic hydrolysis liquid is removed by nanofiltration technology 2 Salt and concentrated solution are spray-dried to obtain silk fibroin peptide powder. The invention has the advantages of large processing capacity, high desalination rate, mild separation conditions, simple process and low energy consumption, can realize industrial production, and prepare high-purity silk fibroin peptide powder for the development of functional food.

Description

technical field [0001] The present invention relates to a preparation method of biologically active peptide-silk fibroin peptide, in particular to a method for preparing silk fibroin peptide by hydrolyzing silk solution by enzymatic method, and then removing high-concentration calcium chloride in the enzymatic hydrolysis solution by nanofiltration technology, It belongs to the field of food biotechnology. Background technique [0002] Silk is a kind of fibrin, composed of silk fibroin and sericin, of which silk fibroin accounts for 70% to 80%, sericin accounts for 20% to 30%, silk fibroin is composed of 18 kinds of amino acids, and about 85% are glycine, alanine amino acid, serine and tyrosine. Silk peptide (silk peptide) is an intermediate product of silk fibroin hydrolysis. Usually, the degummed silk fibroin is dissolved, dialyzed, purified, degraded, concentrated and other processes, and then spray-dried to produce high-purity silk peptide powder. Silk peptide has the s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/02
Inventor 王璋杨瑞金许时婴周凤娟倪莉
Owner JIANGNAN UNIV
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