Small-sized mechanism and equipment for realizing rapid cell disruption

Abstract

The utility model belongs to the technical field of cell disruption, and particularly relates to a small-sized mechanism and equipment for quickly disrupting cells, the small-sized mechanism for quickly disrupting the cells comprises a driving motor, the driving motor can drive a rotating disc to rotate, a high-strength rotating rod is arranged on the periphery of the rotating disc, and the high-strength rotating rod is connected with the driving motor. A high-strength rotating rod is arranged on the upper cover, a striking ball is arranged at the tail end of the high-strength rotating rod and can strike a sample test tube placed on a circumferential hole of the upper cover, and a limiting device covers the sample test tube, so that the sample test tube has a jerk value of 1-2mm in the vertical direction. The utility model provides a small mechanism and equipment for quickly breaking cells.

Description

Technical Field

[0001] The utility model belongs to the technical field of cell disruption, and in particular relates to a small mechanism and equipment for realizing rapid cell disruption. Background Art

[0002] Existing technology:

[0003] Cell disruption technology refers to the use of external force to destroy the cell membrane and cell wall to release the cell contents including the target product components. It is the basis for separating and purifying non-secretory biochemical substances synthesized within cells. Different cells require different methods to disrupt tissues and cells. The disruption methods usually include mechanical disruption, chemical and biochemical osmosis, and physical osmosis. Cell disruption by stirring is also common.

[0004] However, the inventors of this application have found that the above-mentioned prior art has at least the following technical problems:

[0005] However, the existing cell crusher cannot crush cells quickly and effectively using the stirring and crushing method. The existing cell crusher cannot crush cells quickly and effectively when using the stirring and crushing method to crush cells, and the crushing time is long, the efficiency is low, and the practicality is not high.

[0006] The difficulty and significance of solving the above technical problems:

[0007] Therefore, based on these problems, providing a small mechanism and equipment for achieving rapid cell disruption has important practical significance. Utility Model Content

[0008] The purpose of this application is to provide a small mechanism and device for achieving rapid cell disruption in order to solve the technical problems in the prior art.

[0009] The technical solution adopted by the embodiment of the present application to solve the technical problems existing in the known technology is:

[0010] A small mechanism for achieving rapid cell disruption, comprising a drive motor, which can drive a rotating disk to rotate; a high-strength rotating rod is installed on the outer circumference of the rotating disk; a striking ball is installed on the end of the high-strength rotating rod; the striking ball can strike a sample tube placed on a circumferential hole of an upper cover; a limiting device is covered on the sample tube, so that the sample tube has a vertical jump of 1-2 mm.

[0011] The embodiments of the present application may also adopt the following technical solutions:

[0012] In the above-mentioned small mechanism for realizing rapid cell disruption, further, the driving motor is fixed on a motor fixing seat, a cooling fan is installed on the motor fixing seat, and the cooling fan is located below the driving motor.

[0013] In the above-mentioned small mechanism for realizing rapid cell disruption, further, the high-strength rotating rods are fixed to the rotating disk by spring pins and are evenly distributed along the radial direction of the rotating disk.

[0014] In the above-mentioned small-sized mechanism for realizing rapid cell disruption, further, the diameter of the outer circle of the sample test tube is smaller than the diameter of the circumferential holes of the upper cover, and the circumferential holes are evenly distributed in a ring shape.

[0015] A small device for realizing rapid cell disruption, wherein the small device for realizing rapid cell disruption adopts any of the small mechanisms for realizing rapid cell disruption described above.

[0016] In the above-mentioned small device for realizing rapid cell crushing, further, the small device for realizing rapid cell crushing includes a bottom shell, an upper cover and a top cover, the upper cover and the top cover are hinged by a rotating shaft, the motor fixing seat is fixedly connected to the bottom shell, the bottom shell is supported by a silicone suction cup, and a magnetic patch is provided at the front end of the top cover, which can generate electromagnetic attraction with the magnet inside the device to close the top cover.

[0017] In the above-mentioned small-sized device for realizing rapid cell disruption, further, the upper cover has 24 evenly distributed circumferential holes, the limiting device is a foam pressure plate, and the foam pressure plate is fixedly connected to the top cover.

[0018] In the above-mentioned small device for realizing rapid cell disruption, further, the bottom shell is provided with an adapter socket for powering the movement, a power switch for controlling the movement, and a liquid crystal screen.

[0019] One or more technical solutions provided in the embodiments of the present application have at least the following beneficial effects:

[0020] 1. The utility model adopts high-speed and high-frequency continuous striking technology. By continuously striking the sample tube at high speed and high frequency, the cell sample in the sample tube can be quickly broken, which effectively solves the problems of long breaking time, low efficiency and low practicality in the prior art. It has the advantages of saving time, saving detection costs, fast response, simple structure and easy operation. It can also break multiple groups of samples at the same time, saving manpower and having high effect.

[0021] 2. The utility model only needs to pre-set the striking frequency and striking time to easily achieve effective crushing of cell samples of different specifications. The motor speed can be adjusted from 500r / m to 1100r / m, and different striking frequencies can be provided according to different samples (the striking frequency can be obtained by multiplying the speed by the number of striking hammers). At the same time, different striking times can be set. According to different samples, the striking time generally ranges from 200s to 400s.

[0022] 3. The number of striking balls in the utility model is five, and the striking frequency is increased five times based on the motor speed, that is, the sample tube will be struck five times for every rotation of the motor; at the same time, the number of sample tubes placed at the same time in the utility model is twenty-four, which can provide a maximum of twenty-four samples for simultaneous crushing, greatly improving efficiency.

[0023] 4. The utility model does not directly contact the processed sample (the sample is stored in the sample test tube). Therefore, during the entire crushing process, the device will not be contaminated by the sample and can remain clean without cleaning. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] The technical solutions of the embodiments of the present application will be further described in detail below in conjunction with the accompanying drawings, but it should be noted that these drawings are designed only for explanation purposes and are not intended to limit the scope of the present application. In addition, unless otherwise specified, these drawings are intended only to conceptually illustrate the structural configurations described herein and are not necessarily drawn to scale.

[0025] Figure 1 It is a three-dimensional structural schematic diagram of the first embodiment of the utility model.

[0026] Figure 2 It is a schematic diagram of the internal cutaway structure of the first embodiment of the utility model.

[0027] Figure 3 It is a top view of the first embodiment of the present utility model.

[0028] Figure 4 This is a schematic diagram of the placement of sample test tubes in Example 1 of the utility model.

[0029] Figure 5 It is a structural schematic diagram of the third embodiment of the present utility model.

[0030] Figure 6 It is a front view of the third embodiment of the present utility model.

[0031] Figure 7 It is a side view of the third embodiment of the present utility model.

[0032] Figure 8 It is a top view of the third embodiment of the present utility model.

[0033] Fig. 9 It is a back view of the third embodiment of the present utility model.

[0034] In the figure:

[0035] 1. Motor fixing seat, 2. Driving motor, 3. Rotating disk, 4. Spring pin, 5. High-strength rotating rod, 6. Beating ball, 7. Cooling fan, 8. Sample test tube.

[0036] 11. Bottom shell, 12. Upper cover, 13. Top cover, 14. LCD screen, 15. Magnetic patch, 16. Foam pressure plate, 17. Silicone suction cup, 18. Hinge, 19. Adapter socket, 20. Power switch. DETAILED DESCRIPTION

[0037] Under the control of external accessories, the driving motor 2 on the motor fixing seat 1 rotates at high speed, thereby driving the rotating disk 3 fixed thereto to rotate at high speed. The rotating disk is evenly placed and fixed with five high-strength rotating rods 5 through five spring pins 4, and nylon hitting balls 6 are fixed to the ends of the five high-strength rotating rods 5. The hitting ball 6 is a sphere. When the rotating disk 3 rotates at high speed, the hitting balls 6 also rotate at high speed, continuously and evenly hitting the sample test tubes arranged in a circular array. The cell samples in the sample test tubes 8 are quickly broken under the continuous and even impact of the hitting balls 6.

[0038] In order to better understand the above technical solution, the above technical solution will be described in detail below in conjunction with the accompanying drawings and specific implementation methods.

[0039] Example 1

[0040] The small mechanism for realizing rapid cell disruption in this embodiment includes a motor fixing seat 1, a driving motor 2, a rotating disk 3, a spring pin 4, a high-strength rotating rod 5, a nylon hitting ball 6, a cooling fan 7 and an external sample tube 8.

[0041] Working process:

[0042] When in use, first fix the drive motor 2 on the motor fixing base 1, and reliably connect the rotating disk 3 to the drive motor 2, assemble the spring pin 4, high-strength rotating rod 5, nylon hitting ball 6 and other components in place, connect the power provided by the external accessories to power the drive motor 2, and the drive motor 2 starts to be in working state.

[0043] First, the driving motor 2 drives the rotating disk 3 to rotate at high speed, and the high-strength rotating rod 5 and the nylon hitting ball 6 also rotate at high speed following the rotating disk 3. At this time, the nylon hitting ball 6 is in an unloaded state.

[0044] Secondly, the sample test tubes 8 are placed in the evenly distributed circumferential holes of the test tube holder 9, and the upper cover 12 can hold up to 24 groups of sample test tubes 8. After the sample test tubes 8 are placed in place, the device is started, and the nylon hitting ball 6 starts to rotate at high speed. During the high-speed rotation process, it will continuously hit each group of sample test tubes 8. After the motor rotates at high speed, the sample test tubes 8 will jump in the vertical direction in their respective circumferential holes after being hit, and will fall back vertically downward after the hitting ends.

[0045] The sample tube 8 is limited by a limit device above it, and the sample tube 8 has a vertical runout of 1-2 mm. The outer diameter of the sample tube 8 itself is smaller than the circumferential hole of the upper cover 12, and the sample tube 8 itself will also freely shake in the horizontal direction in the circumferential hole, but the shaking amount is smaller than that in the vertical direction. There are five nylon hitting balls 6, and the same group of sample tubes 8 is hit five times for each rotation of the driving motor 2.

[0046] The speed and working time of the driving motor 2 are set, and after the driving motor 2 is in a stopped state, the sample test tubes 8 on the upper cover 12 can be taken out. A new set of sample test tubes 8 is placed again to start the next round of testing.

[0047] When the drive motor 2 is in the starting working state, the heat dissipation fan 7 at the lower part of the drive motor 2 is started at the same time to discharge the heat generated by the high-speed rotation of the drive motor 2.

[0048] When not in use, the driving motor 2 and the cooling fan 7 are first stopped, and then the upper cover 12 and the sample tube 8 are removed. At the same time, a small mechanism for realizing rapid cell disruption is stored in place for the next use.

[0049] Example 2

[0050] This embodiment provides prefabricated parameter settings for the striking frequency and striking time of different samples, as shown in the following table for reference:

[0051]

[0052] This embodiment can effectively crush cell samples of different specifications, and the corresponding function can be easily achieved by adjusting the motor speed and crushing time. It has a simple structure, is easy to operate, and can crush multiple groups of samples at the same time, saving manpower, having obvious effects, and is worthy of promotion.

[0053] Example 3

[0054] The small device for realizing rapid cell disruption of this embodiment includes a bottom shell 11, an upper cover 12 and a top cover 13, and adopts the small mechanism for realizing rapid cell disruption of the first embodiment. The motor fixing seat 1 of the first embodiment is fixedly connected to the bottom shell 11 of the whole device. The limiting device above the sample test tube 8 is a foam pressure plate 16, which allows the sample test tube 8 to have a jump of 1-2 mm in the vertical direction.

[0055] Before use, the top cover 13 is in a closed state.

[0056] When in use, the whole device is first fixed on a flat workbench through four silicone suction cups 17 at the bottom of the bottom shell 11. The external power adapter accessory is assembled in place, and the internal movement is powered through the adapter socket 19. The power switch 20 is turned on, the device starts, and the LCD screen 14 displays the device's operating interface.

[0057] At this time, the top cover 13 is opened to be in an open state, and the rotating shaft 18 hinges the top cover 13 with the upper cover 12 , and the rotating shaft 18 ensures that the top cover 13 can rotate around the central axis of the rotating shaft 18 when the upper cover 12 is fixed.

[0058] The cell sample tubes 8 to be broken are placed in the circumferential holes of the upper cover 12 . The upper cover 12 can hold a maximum of 24 sample tubes 8 .

[0059] After all the sample tubes 8 are in place, the top cover 13 is closed to make it in a closed state. The magnetic patch 15 at the front end of the top cover 13 generates electromagnetic attraction with the permanent magnet embedded inside the device to close the top cover 13, ensuring that the top cover will not open automatically during the operation of the whole machine, and preventing the sample tubes 8 from moving up and down. The foam pressing plate 16 in the middle of the top cover 13 covers all the sample tubes 8, ensuring that the amount of movement between the sample tubes 8 and the top cover 13 is constant and stable during the operation of the device.

[0060] After setting the operating parameters on the LCD screen 14 and starting the device, the movement inside the device starts to operate and the cell sample in the sample test tube 8 is broken.

[0061] When not in use, turn off the power switch 20 and open the top cover 13 again to put it in the open state. At this time, the next group of sample tubes 8 can be replaced and the next group of sample crushing can be repeated.

[0062] Existing cell disruption equipment cannot quickly and effectively disrupt cells using a stirring disruption method. This embodiment can continuously and evenly impact the sample tubes arranged in a circular array, and the cell samples in the sample tubes are quickly disrupted under the continuous and even impact of the grinding balls.

[0063] In summary, the utility model provides a small mechanism and device for realizing rapid cell disruption.

[0064] The above embodiments describe the present invention in detail, but the above contents are only preferred embodiments of the present invention and cannot be considered to limit the scope of implementation of the present invention. All equivalent changes and improvements made within the scope of application of the present invention shall still fall within the scope of the patent coverage of the present invention.

Claims

1. A small mechanism for achieving rapid cell disruption, characterized in that: The small mechanism for achieving rapid cell disruption includes a drive motor, which can drive a rotating disk to rotate. A high-strength rotating rod is installed on the outer circumference of the rotating disk. A striking ball is installed on the end of the high-strength rotating rod. The striking ball can hit a sample tube placed on the circumferential hole of the upper cover. The sample tube is covered with a limiting device, so that the sample tube has a vertical jump of 1-2 mm.

2. The small-sized mechanism for achieving rapid cell disruption according to claim 1, characterized in that: The driving motor is fixed on a motor fixing seat, a cooling fan is installed on the motor fixing seat, and the cooling fan is located below the driving motor.

3. The small-sized mechanism for achieving rapid cell disruption according to claim 1, characterized in that: The high-strength rotating rods are fixed to the rotating disk through spring pins and are evenly distributed along the radial direction of the rotating disk.

4. The small-sized mechanism for achieving rapid cell disruption according to claim 1, characterized in that: The diameter of the outer circle of the sample test tube is smaller than the aperture of the circumferential holes on the upper cover, and the circumferential holes are evenly distributed in a ring shape.

5. A small device for achieving rapid cell disruption, characterized in that: The small device for achieving rapid cell disruption adopts the small mechanism for achieving rapid cell disruption described in any one of claims 1-4.

6. The small device for achieving rapid cell disruption according to claim 5, characterized in that: The small device for achieving rapid cell disruption includes a bottom shell, an upper cover and a top cover, wherein the upper cover and the top cover are hinged via a rotating shaft, a motor fixing seat is fixedly connected to the bottom shell, the bottom shell is supported by a silicone suction cup, and a magnetic patch is provided at the front end of the top cover, which can generate electromagnetic attraction with a magnet inside the device to close the top cover.

7. The small device for achieving rapid cell disruption according to claim 5, characterized in that: The upper cover is provided with 24 evenly distributed circumferential holes, and the limiting device is a foam pressing plate, which is fixedly connected to the top cover.

8. The small device for achieving rapid cell disruption according to claim 6, characterized in that: The bottom shell is provided with an adapter socket for supplying power to the movement, a power switch for controlling the movement, and a liquid crystal screen.

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